ISSN:
0170-2041
Keywords:
Carbohydrates
;
Galactose, 2-acetamido-2-deoxy-D-
;
O-Glycoproteins
;
Glycosyltransferases
;
Enzymes
;
Chemistry
;
Organic Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Building Units of Oligosaccharides, CII. - Synthesis of Modified Derivatives of 2-Acetamido-2-deoxy-D-galactose for the Examination of Substrate Specificities of Core 1-β3-Gal-Transferase and Core 3-β3-GlcNAc-Transferase Involved in the Biosynthesis of O-GlycoproteinsThe 2-, 3-, 4-, and 6-deoxy derivatives of benzyl 2-acetamido-2-deoxy-α-D-galactopyranoside (7) have been synthesized to test substrate specificities of glycosyltransferases involved in the biosynthesis of O-glycoproteins. The core 1-β3-Gal-transferase dose not require the 6-hydroxyl group for activity in contrast to the glycosylation reaction of the core 3-β3-GlcNAc-transferase which requires the 6-hydroxyl group. Compounds with reactive groups substituting the 6-hydroxyl group such as the diazirine 45 could be suitable for photolabeling of the former enzyme. A column for affinity chromatography with the 6-deoxy derivative as the ligand could be useful to separate the two enzymes during purification.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jlac.1992199201124
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