ISSN:
1573-4986
Keywords:
cancer cells
;
glycan:sulfotransferases
;
kinetic properties
;
lymph nodes
;
specificities
;
tumors
;
AA/CP, acrylamide copolymer
;
Al., allyl
;
AAL, Aleuria aurantia lectin
;
BAL., British anti-lewisite (2,3-dimercaptoenthanol)
;
Bn, benzyl
;
CALN, calf axial lymph node
;
CMLN, calf mesenteric lymph node
;
CEA, carcinoembryonic antigen
;
Me, methyl
;
PAPS, 3′phosphoadenosine 5′-phosphosulfate
;
PNA, peanut agglutinin
;
pNP, p-nitrophenyl
;
RM, reaction mixture
;
TLC, thin layer chromatography
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract We found earlier in human breast and colon tumors, an augmented level of Gal : 3-O-sulfotransferase activities showing, respectively, an acceptor preference to blood group T-hapten (Group A enzymes) or Galβ1,4GlcNAc (Group B enzymes) on the mucin Core 2 structure [Chandrasekaran EV, Jain RK, Vig R, and Matta KL (1997) Glycobiology 7: 753–68]. The present study reports these enzyme activities in human tumor cell lines and normal tissues. The human colon tumor epithelial cell lines, akin to their parent tumors, express Group B enzyme activity. The acceptor specificity and kinetic properties, such as divalent metal ion activation and pH dependent activity profile, of the colon cancer line LS180 enzyme activity are identical to those of colon tissue specimens. Consistent with breast tumor specimens, the Group A enzyme activity is present in human breast tumor epithelial cell lines, with some exceptions. The Gal : 3-O-sulfotransferases show specific binding to Aleuria aurantia lectin, suggesting the presence of asparagine linked carbohydrate chains containing an inner core α1,6-fucosyl residue on these enzymes. Calf lymph nodes contain GlcNAc : 6-O-sulfotransferase as well as Group A Gal : 3-O-sulfotransferase activities, which differ in pH dependent profiles, pH optima (7.6 and 7.0, respectively) and the influence of Mn2+.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1007074005371
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