ISSN:
1365-2036
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
An increased permeability to sugars is found in the intestine of untreated patients with coeliac disease after oral ingestion.〈section xml:id="abs1-2"〉〈title type="main"〉Aim:To test whether in vitro permeability resembles in vivo permeability tests and whether an in vitro gliadin gluten challenge could be performed by an in vitro permeability test.〈section xml:id="abs1-3"〉〈title type="main"〉Methods:We measured in vivo (urinary excretion after sucrose–lactulose–mannitol ingestion) and in vitro permeability (by mini-Ussing chambers) in 25 healthy controls, 12 relatives of coeliac disease patients, 19 treated, eight partly treated and 16 untreated patients with coeliac disease.〈section xml:id="abs1-4"〉〈title type="main"〉Results: In vivo sugar permeability was increased in nearly all coeliac patients. Additionally, in vitro permeability to lactulose (P=0.0007), mannitol (P=0.004) and sucrose (P=0.042) was higher in untreated patients with coeliac disease. It correlated with in vivo permeability (sucrose τ=0.61, P=0.006; lactulose τ=0.41, P 〈 0.0001; mannitol τ=– 0.56, P=0.62) and was dependent on mucosal damage. An in vitro gliadin challenge over 24 h could not significantly change in vitro permeability in treated patients with coeliac disease.〈section xml:id="abs1-5"〉〈title type="main"〉Conclusions:An in vitro permeability test capable of measuring elevated permeability in coeliac mucosa was described, but this test cannot replace oral gluten challenge by in vitro gliadin incubation.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1046/j.1365-2036.2001.01034.x
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