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  • 1
    Electronic Resource
    Electronic Resource
    Novel Isoforms of Rat Brain Fructose 6-Phosphate 2-Kinase/Fructose 2,6-Bisphosphatase Are Generated by Tissue-Specific Alternative Splicing (1997)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 69 (1997), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Fructose 6-phosphate 2-kinase/fructose 2,6-bisphosphatase catalyzes the synthesis and degradation of fructose 2,6-bisphosphate, which is the most potent activator of glycolysis. We have shown previously the occurrence of a partial cDNA (RB7) encoding the catalytic core domain of a novel brain-type isozyme. To elucidate the full-length sequence of RB7 cDNA, we have carried out cDNA cloning using the 3′- and 5′-rapid amplification cDNA ends method and have isolated eight isoforms from rat brain. The cDNA sequences encoding the 5′-untranslated region, the amino-terminal domain, and the catalytic core domain were identical among all the isoforms. However, heterogeneity of the carboxyl-terminus was found by sequence analysis. This heterogeneity was shown not to have resulted from transcription of multiple genes, as Southern blot and genomic sequence analysis revealed that the gene was a single copy in the rat genome. It is likely that these transcripts represent splice variants of the gene. High-level expression of the gene was also observed by northern blot analysis in skeletal muscle. However, the pattern of alternative splicing was different from that of brain, and only four isoforms were detected in skeletal muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.1997.69010001.x
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of an Escherichia coli flagellin gene, hag48, in the presence of a Salmonella H1-repressor (1985)
    Springer
    Molecular genetics and genomics 201 (1985), S. 133-135 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An Escherichia coli K12 flagellin gene, hag48, was found to be expressed in the presence of the Salmonella rh1 gene product. The strains which had hag48 on a chromosome or on an F′ factor were constructed from strains H2-e,n,x on-off rh1 + and fixed H2-e,n,x on rh1 + in which rh1 + is contranscribed with H2 in its “on” state. Motility of these strains in semisolid medium was inhibited by anti-H48 serum and motile clones (swarms) that escaped from it were hag mutants in case of the hag48 e,n,x on-off strain tested. H48 flagellin was detected by electrophoresis, though its amount was less than e,n,x flagellin, from all the strains that were nonmotile in the presence of anti-H48 serum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397999
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation and characterization of Escherichia coli hag operator mutants whose hag48 expression has become repressible by a Salmonella H1 repressor (1989)
    Springer
    Molecular genetics and genomics 216 (1989), S. 44-50 
    Details
    ISSN: 1617-4623
    Keywords: Flagellin gene ; H1 repressor ; hag48 operator mutations ; Transcriptional initiation site ; Transcriptional terminator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The expression of an Escherichia coli K12 flagellin gene, hagA48, is insensitive to the Salmonella H1 repressor (rh1 −).By selecting merodiploid cells H2-rh1 on-off/F′hag48 for motility in the presence of anti-H48 serum, mutants which had escaped from inhibition by the serum because of repression of their hag48 expression by rh1 + were isolated. Their nucleotide sequences were examined in the region containing the promoter, the position of which was confirmed by S1 nuclease analysis of the transcriptional initiation site. The two independently isolated mutants had the same heptamer insertion AGACGAT at a site overlapping with the promoter sequence, creating a putative operator sequence homologous to Salmonella H1, but not to H2. Other candidates for operator mutants had reduced flagellar synthesis because of mutations between the transcriptional and translational initiation sites or in the structural gene. The sequence analysis also revealed a repetitive extragenic palindrome (REP) consensus sequence and a transcriptional terminator of hag48 in a small, functionally unknown open reading frame (ORF).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332229
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  • 4
    Electronic Resource
    Electronic Resource
    Adaptive significance of sprouting ofEuptelea polyandra, a deciduous tree growing on steep slopes with shallow soil (1995)
    Springer
    Journal of plant research 108 (1995), S. 377-386 
    Details
    ISSN: 1618-0860
    Keywords: Basal dormant bud ; Ground-surface disturbance ; Repair of tree form ; Stool development ; Uprooting ; Vegetative reproduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We investigated growth characteristics ofEuptelea polyandra Sieb. et Zucc. (Eupteleaceae), a Japanese endemic deciduous tree species growing on unstable ground such as that of very steep slopes with thin soil.Euptelea polyandra began to sprout at the juvenile stage and had a multiple-stemmed tree form. There was a positive correlation between diameter of the maximumsized stem within a stool (DMS) and the number of stems within the stool. Many stools had suffered from disturbances as shown by the fact that uprooting scars were found on 31.4% and 42.4%, respectively, of the stools of the two populations studied. Sprouting played a significance role in repairing damaged stems and stools, and at least 15.5% and 18.2% of the stools of the two populations, respectively, had apparently avoided death by sprouting. Sprouted stems gradually inclined with the increase in their relative sizes within each stool, and this seemed to facilitate the establishment of younger sprouted stems. The small younger sprouted stems had their own roots. There were dormant buds on stems which originated from axillary buds, and secondary dormant buds occurred by branching. The total number of dormant buds in a stool increased with DMS. It is concluded thatE. polyandra accumulates dormant buds for sprouting in order to respond to disturbances quickly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02344363
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  • 5
    Electronic Resource
    Electronic Resource
    Vegetation pattern and microtopography on a landslide scar of Mt Kiyosumi, central Japan (1993)
    Springer
    Ecological research 8 (1993), S. 47-56 
    Details
    ISSN: 1440-1703
    Keywords: disturbance regime ; Euptelea polyandra Sieb. et Zucc. ; landslide ; microtopography ; vegetation pattern analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Vegetation pattern and microtopography were examined on a mountain slope with a rotational type landslide scar on Mt Kiyosumi, central Japan. Similarities of distribution patterns among 55 woody species were calculated using Cole's species association coefficient, and based on them, seven vegetation units were classified using cluster analysis and principal coordinates analysis. The seven vegetation units coincide with seven microtopographical facets at 101 to 102 m2 order. Furthermore, these vegetation units were grouped into three higher categories by reciprocal averaging and principal coordinates analysis. They were ridge slopes, surrounding slopes and landslide slopes. The three categories were arranged in the above-mentioned order based on similarity in floristic composition. In the ridge slopes, late-successional trees and deciduous trees had high relative basal areas. In the surrounding slopes,Euptelea polyandra and other deciduous trees had high relative basal areas. In the landslide slopes,E. polyandra and deciduous shrubs had high relative basal areas. The density and the age distributions ofE. polyandra, a typical pioneer tree which invades disturbed sites, suggested that the severity of soil surface disturbances increase in this order. The disturbance regime explains the vegetation pattern on the study site, where the rotational type landslide had occurred.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02348606
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  • 6
    Electronic Resource
    Electronic Resource
    Topographical pattern of the forest vegetation on a river basin in a warm-temperate hilly region, central Japan (1994)
    Springer
    Ecological research 9 (1994), S. 269-280 
    Details
    ISSN: 1440-1703
    Keywords: disturbance ; geomorphic process ; topographical unit ; vegetation pattern analysis ; warmtemperate forest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationship between tree distribution and topography was examined in a small river basin (3.4 ha) comprising a complex mosaic of topographical units at 102 to 103 m2 order, each of which had a shallow valley bordered by small ridges or breaks of slopes. Twenty-five major woody species were divided into two groups (groups A and B) based on a cluster analysis using the distribution data in the basin. Group A, which mainly consisted of early-successional species, was distributed around the valley sites of the topographical units, while group B, which mainly consisted of late-successional species, was distributed around the ridge sites of the topographical units. This vegetation pattern coincided with erosional condition in the basin. That is, the valley sites were eroded more actively than the ridge sites, as soil depth tended to be thin in the valley sites and thick in the ridge sites, and because large (canopy) trees were restricted in the ridge sites. There was no tendency that group B was replacing group A, and hence it was suggested that repeated disturbance by slope failures or small-scale shallow landslides have prevented compositional change from the early-successional (group A) to the late-successional (group B) species by preventing the invasion of the latter into valley sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02348413
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