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  • 1
    Electronic Resource
    Electronic Resource
    Temperature-Sensitive Chlamydomonas Mutants Manifesting Flagellar Regression at a Restrictive Temperature (1979)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 26 (1979), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The regeneration kinetics of Chlamydomonas reinhardtii mutants TS-6 and TS-79, whose flagella were mechanically amputated, indicated that the flagellar precursor in cytoplasm was used for regeneration when cycloheximide was present. The TS-6 cells rendered nonflagellate by regression at 35 C did not regenerate in the presence of cycloheximide, indicating that the precursor was inactivated by the high temperature. Neither mutant was able to use the absorbed flagellar components for regeneration in the presence of cycloheximide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1979.tb02767.x
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular characterization of intact, but cryptic, flagellin genes in the genus Shigella (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 12 (1994), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Flagellin genes (fliC) were detected in two species of the genus Shigella. The fliCSF gene cloned from Shigella flexneri produced normal-type flagella in an Escherichia coli δ fliC strain while the fliCSS genes from two Shigella sonnei strains produced curly-type flagella and their expression is repressible by Salmonella FljA repressor. The fliCSF gene (1650bp) shared high similarity with the E. coli fliCE gene not only in the 5′ and 3′ constant sequences but also in the upstream and downstream sequences. The fliCS genes (1572 bp) shared high similarity with the Salmonella typhimurium fliCs gene in the operator and 3’constant sequences and also shared high similarity with the fliCE gene in the downstream sequence, suggesting that the fliC gene has undergone horizontal transfer and recombination. Differences In nucleotide sequences of the central variable regions among the four fliC genes, including fliCE and fliCS, suggest that they started differentiation in each lineage approximately 80 million years ago. Loss of motility in Shigella seems to be evolutionarily a recent event.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1994.tb01016.x
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  • 3
    Electronic Resource
    Electronic Resource
    Surface Treatment of Aluminum Alloy with Laser Irradition to Increase Wear Resistance (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 539-543 (Mar. 2007), p. 404-410 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Laser irradiating process with Nd-YAG laser is investigated in order to improve theadhesion and wear resistance of low pressure plasma sprayed layer on the surface of aluminumextruded shape using the atomized powder of Al-50mass%Fe, Al-15mass%Fe-17mass%Si andAl-50mass%Si. The effect of pulse energy of laser beam on the microstructure, micro hardnessand wearing rate of these laser irradiated layers are evaluated. Laser irradiated layers haveappeared more smooth surface and better adhesion than as sprayed layer. Depth profile of microhardness where laser irradiated is respectively kept constant. In the microstructure of laserirradiated layer of Al-50mass %Fe, fine needle-like Al3Fe and massive Al2Fe are dispersed. Microhardness increases with decrease of the pulse energy of laser beam However, the wearing rate oflaser irradiated layer increases due to the initiation of cracking. In the microstructure of laserirradiated layer of Al-15mass%Fe-17mass%Si, ultra fine needle-like and massive (Al, Fe, Si)ternary crystals are aggregated. In the microstructure of laser irradiated layer of Al-50mass%Si,ultra fine hyper-eutectic structure is observed. Micro hardness of these layers are HV250-350,HV150-200, respectively and wearing rate of these layer are 1/7 or less than anodized surface
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/14/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.539-543.404.pdf
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of an Escherichia coli flagellin gene, hag48, in the presence of a Salmonella H1-repressor (1985)
    Springer
    Molecular genetics and genomics 201 (1985), S. 133-135 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An Escherichia coli K12 flagellin gene, hag48, was found to be expressed in the presence of the Salmonella rh1 gene product. The strains which had hag48 on a chromosome or on an F′ factor were constructed from strains H2-e,n,x on-off rh1 + and fixed H2-e,n,x on rh1 + in which rh1 + is contranscribed with H2 in its “on” state. Motility of these strains in semisolid medium was inhibited by anti-H48 serum and motile clones (swarms) that escaped from it were hag mutants in case of the hag48 e,n,x on-off strain tested. H48 flagellin was detected by electrophoresis, though its amount was less than e,n,x flagellin, from all the strains that were nonmotile in the presence of anti-H48 serum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397999
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and characterization of Escherichia coli hag operator mutants whose hag48 expression has become repressible by a Salmonella H1 repressor (1989)
    Springer
    Molecular genetics and genomics 216 (1989), S. 44-50 
    Details
    ISSN: 1617-4623
    Keywords: Flagellin gene ; H1 repressor ; hag48 operator mutations ; Transcriptional initiation site ; Transcriptional terminator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The expression of an Escherichia coli K12 flagellin gene, hagA48, is insensitive to the Salmonella H1 repressor (rh1 −).By selecting merodiploid cells H2-rh1 on-off/F′hag48 for motility in the presence of anti-H48 serum, mutants which had escaped from inhibition by the serum because of repression of their hag48 expression by rh1 + were isolated. Their nucleotide sequences were examined in the region containing the promoter, the position of which was confirmed by S1 nuclease analysis of the transcriptional initiation site. The two independently isolated mutants had the same heptamer insertion AGACGAT at a site overlapping with the promoter sequence, creating a putative operator sequence homologous to Salmonella H1, but not to H2. Other candidates for operator mutants had reduced flagellar synthesis because of mutations between the transcriptional and translational initiation sites or in the structural gene. The sequence analysis also revealed a repetitive extragenic palindrome (REP) consensus sequence and a transcriptional terminator of hag48 in a small, functionally unknown open reading frame (ORF).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332229
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  • 6
    Electronic Resource
    Electronic Resource
    A naturally occurring large chromosomal inversion in Escherichia coli K12 (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 376-379 
    Details
    ISSN: 1617-4623
    Keywords: Escherichia coli K12 ; Large inversion ; Gene rearrangement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Strain 1485IN and its derivatives were found to have a large inversion extending to about 35% of the chromosome. Because of this, the question arose as to whether 1485IN had arisen from an Escherichia coli strain other than K12. However, 1485IN had a flagellar antigen and a restriction-modification system indistinguishable from those of W3110, a major line of K12, and had retained an amber suppressor and λ sensitivity that are characteristics of W1485 from which this strain seems to have arisen. Strain 1485IN had acquired proline auxotrophy, but showed the same growth rate as W1485 in nutrient broth at 37°C. Interrupted matings with Hfr strains of 1485IN revealed a gene arrangement of nalA-gal-trp-his-lac-proA-thrleu-ilv, in which gal, trp, and his were on the inverted segment. The termini of the inversion were inferred to be situated between tsx (9.5 min) and purE (12 min) and between his (44 min) and cdd (46.5 min).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00430454
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  • 7
    Electronic Resource
    Electronic Resource
    Nucleotide sequence and regulated expression of the Salmonella fljA gene encoding a repressor of the phase 1 flagellin gene (1993)
    Springer
    Molecular genetics and genomics 236 (1993), S. 260-266 
    Details
    ISSN: 1617-4623
    Keywords: ftjA nucleotide sequence ; FljA repressor ; Intercistronic terminator ; Salmonella abortus-equi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleotide sequence of Salmonella abortus-equi fljA, which together with the phase 2 flagellin gene constitutes the fljBA operon and encodes the repressor for the phase 1 flagellin gene fliC, was determined. The repressor was predicted to be a basic protein consisting of 179 amino acid residues (Mr = 20419 Da) encoded by ORFII. This was confirmed by the fact that host fliC is repressed by plasmid-encoded ORFII, which indeed expresses a 20 kDa product as determined by urea SDS-polyacrylamide gel electrophoresis. An amino acid sequence capable of forming a helix-turn-helix type of structure was predicted in the C-terminal region of FljA. A rho-independent intercistronic terminator was detected between fljB and ftjA. Chloramphenicol acetyltransferase (CAT) assays of fusions indicated that the terminator is capable of reducing expression of fljA to the level of a few percent, relative to fljB in broth cultures and to 1 % in M9 glycerol cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00277121
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