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  • 1
    Electronic Resource
    Electronic Resource
    Isolation and characterization of RAPD-based markers linked to the beet cyst nematode resistance locus (Hs1 pat-1) on chromosome 1 of B. patellaris (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 885-891 
    Details
    ISSN: 1432-2242
    Keywords: Randomly amplified polymorphic DNA (RAPD) ; Deletion mapping ; Sequence Tagged Site (STS) ; Monosomic fragment additions ; Beet cyst nematode resistance ; Heterodera schachtii Schm ; Beta patellaris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A beet cyst nematode (BCN)-resistant telosomic addition of B. patellaris chromosome 1 in B. vulgaris was used to isolate 6 RAPD markers linked to the BCN resistance locus Hs1 pat-1. Southern analysis showed that the analyzed RAPD products contain either low-, middle or high-repetitive DNA. The relative positions of the random amplified polymorphic DNA (RAPD) markers and of the restriction fragment length polymorphism (RFLP) loci corresponding to the low-repetitive RAPD products were determined by deletion mapping using a panel of seven nematode-resistant B. patellaris chromosome-1 fragment additions. One RAPD marker, OPB11800, was found to be present in two copies on the long arm telosome of B. patellaris chromosome 1. These copies are closely linked to the BCN resistance gene and flank the gene on both sides. On the basis of the nucleotide sequence of OPB11800, sequence-tagged site (STS) primers were developed that amplify specific fragments derived from the two OPB11800 loci. These STS markers can be used in the map-based cloning of the BCN gene, as they define start and finishing points of a chromosomal walk towards the Hs1 pat-1 locus. Two copies of the middle-repetitive OPX21100 marker were mapped in the same interval of the deletion mapping panel as the resistance gene locus and thereby belong to the nearest markers as yet found for the BCN gene in B. patellaris.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222027
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  • 2
    Electronic Resource
    Electronic Resource
    Construction of a YAC library from a Beta vulgaris fragment addition and isolation of a major satellite DNA cluster linked to the beet cyst nematode resistance locus Hs1 pat−1 (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 426-434 
    Details
    ISSN: 1432-2242
    Keywords: Nematode resistance Pulsed field electrophoresis ; Satellite DNA ; Sugar beet Yeast artificial chromosome library
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A YAC library was constructed from the Beta vulgaris fragment addition AN5-203b. This monosomic fragment addition harbors an approximate 12-Mbp fragment of B.patellaris chromosome 1 accomodating the Hs1 pat-1 conferring resistance to the beet cyst nematode (Heterodera schachtii). The YAC library consists of 20,000 YAC clones having an average size of 140 kb. Screening with organelle-specific probes showed that 12% of the clones contain chloroplast DNA while only 0.2% of the clones hybridizes with a mitochondrial specific probe. On the basis of a sugar beet haploid genome size of 750 Mbp this library represents 3.3 haploid genome equivalents. The addition fragment present in AN5-203b harbors a major satellite DNA cluster that is tightly linked to the Hs1 pat-1 locus. The cluster is located on a single 250-kb EcoRI restriction fragment and consists of an estimated 700–800 copies of a 159-bp core sequence, most of which are arranged in tandem. Using this core sequence as a probe, we were able to isolate 1 YAC clone from the library that contains the entire 250-kb satellite DNA cluster.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225377
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  • 3
    Electronic Resource
    Electronic Resource
    Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 of B. patellaris and B. procumbens (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 459-466 
    Details
    ISSN: 1432-2242
    Keywords: Molecular markers Beet cyst nematode resistance ; Hs1 Heterodera schachtii Schm ; Satellite DNA Monosomic fragment additions Beta ; Pulsed-field gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract New members of a satellite DNA family (Sat 121), specific for wild beets of the section Procumbentes of the genus Beta, were isolated. Sequence analysis showed that the members of Sat-121 fall into two distinct classes. The organization of Sat-121 in the vicinity of a beet cyst nematode (Heterodera schachtii Schm.) resistance locus (Hs1) in B. patellaris and B. procumbens was investigated by pulsed-field gel electrophoresis (PFGE) using DNA from a series of resistant monosomic fragment additions, each containing an extra chromosome fragment of B. patellaris chromosome-1 (pat-1) in B. vulgaris. In this way several clusters of Sat-121 flanking the Hs1 pat-1 locus were identified. In nematode resistant diploid introgressions (2n=18), which contain small segments of B. procumbens chromosome-1 (pro-1) in B. vulgaris, only two major Sat-121 clusters were detected near the Hs1 pro-1 locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225381
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation of DNA markers linked to a beet cyst nematode resistance locus in Beta patellaris and Beta procumbens (1992)
    Springer
    Molecular genetics and genomics 235 (1992), S. 432-440 
    Details
    ISSN: 1617-4623
    Keywords: Heterodera schachtii ; Beta vulgaris ; Monosomic additions ; Molecular markers ; Disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In cultivated beet no useful level of resistance of the beet cyst nematode (BCN) Heterodera schachtii Schm. has been found, unlike the situation in wild species of the section Procumbentes. Stable introgression of resistance genes from the wild species into Beta vulgaris has not been achieved, but resistant monosomic additions (2n =18 + 1), diploids of B. vulgaris with an extra alien chromosome carrying the resistance locus, have been obtained. Here we describe a new series of resistant monosomic fragment addition material of B. patellaris chromosome 1 (pat-1). We further describe the cloning of a single-copy DNA marker that specifically hybridizes with a monosomic addition fragment of approximately 8 Mb (AN5-90) carrying the BCN resistance locus. This marker and another fragment-specific, single-copy DNA marker probably flank the BCN locus on the addition fragment present in the AN5-203 material, which is approximately 19 Mb in size. Furthermore, several specific repetitive DNA markers have been isolated, one of which hybridizes to AN5-90 and also to DNA from a smaller DNA segment of Beta procumbens, present in line B883, carrying a BCN resistance locus introgressed into the B. vulgaris genome. This suggests that the specific repetitive marker is closely linked to the BCN locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279390
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    Paper (German National Licenses)
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