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  • 1
    Electronic Resource
    Electronic Resource
    Expression of different p63 variants in healing skin wounds suggests a role of p63 in reepithelialization and muscle repair (2005)
    Oxford, UK; Malden, USA : Blackwell Science Inc
    Wound repair and regeneration 13 (2005), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Healing of skin wounds in mammals involves partial reconstruction of the dermis and coverage of the injured site by keratinocytes. The latter process is achieved by extensive migration and hyperproliferation of keratinocytes at the wound rim. Because the p53 protein family member p63 is expressed in human hyperproliferative epidermis, this study determined whether enhanced keratinocyte proliferation correlates with the expression of p63. Therefore, we investigated the temporal and spatial distribution of four major variants of the p63 transcription factor—TAp63α, TAp63γ, ΔNp63α and ΔNp63γ—during normal skin wound healing in mice. Transcripts encoding amino-terminally truncated ΔNp63 variants were found at high levels in basal and suprabasal keratinocytes of the hyperproliferative wound epithelium. Interestingly, TAp63 variants, which include the conserved transactivation domain TA at their amino-terminus, were also expressed in wound keratinocytes as well as at the edge of the injured subcutaneous muscle panniculus carnosus. These findings suggest splice-variant specific functions of p63 in reepithelialization and muscle repair.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2005.130106.x
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  • 2
    Electronic Resource
    Electronic Resource
    Quantitation of Vasopressin mRNA and Oxytocin mRNA in Hypothalamic Nuclei by Solution Hybridization Assays (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 47 (1986), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Concentrations of vasopressin (VP) precursor and oxytocin (OT) precursor mRNA were measured in magno-cellular cell groups of the rat hypothalamus by newly developed solution hybridization assays. The assays employed single-stranded 35S-labeled VP-specific and OT-specific DNA probes that were prepared by primer extension on re-combinant M13 DNA templates. Solution hybridization assays were standardized by known amounts of cloned DNA. The detection limit was 〈1 pg DNA equivalent of the respective mRNA. In total RNA preparations of microdis-sected supraoptic nucleus (SON) mean (SEM) basal levels of 1.370.18pgVP mRNA and 1.950.I4pgOT mRNA were measured. RNA of the microdissected paraventricular nucleus (PVN) contained 0.35 ± 0.02 pg VP mRNA and 1.77 ± 0.15 pg OT mRNA. Elevation of plasma osmolality induced by drinking of 2% saline for 25 days resulted in a 1.85-fold increase in VP mRNA levels of the SON and a 1.6-fold increase in VP mRNA levels of the PVN. The solution hybridization assays are suitable tools to study the regulation of VP and OT mRNAs in magnocellular neurons of the brain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1471-4159.1986.tb13093.x
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  • 3
    Electronic Resource
    Electronic Resource
    Vasopressin Expression in Cultured Neurons is Stimulated by Cyclic AMP (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neuroendocrinology 2 (1990), S. 0 
    Details
    ISSN: 1365-2826
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Vasopressin and oxytocin genes are expressed in mutually exclusive sets of magnocellular neurons in the hypothalamus. Cell specificity and regulation are probably controlled by extra- and intracellular signals acting on one or the other gene. In order to identify factors that regulate peptide expression, we have used primary dissociated cultures derived from 14-day old foetal rats. Vasopressin expression was monitored by combined immunocytochemistry and in situ hybridization. Treatment of cultures with forskolin and/or the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX), both of which result in elevated intracellular cyclic AMP levels, increased the numbers of vasopressin-expressing cells up to 10-fold. The specific Vasopressin messenger ribonucleic acid accumulation was verified quantitatively by ribonuclease protection assays. Forskolin and IBMX did not change the levels of the general neuronal markers, neuron-specific enolase and synaptophysin, suggesting that the effect of these drugs was specific for vasopressin-expressing cells. The drugs were not mitogenic for magnocellular neurons. Furthermore, their effect was not mediated trans-synaptically, as the drugs were also effective in cultures grown in low Ca2+/high Mg2+ medium, as well as in cultures treated with either tetanus toxin or tetrodotoxin. The presence of putative response elements for the transcription factor AP-2 in the 5’promoter regions of all vasopressin genes sequenced so far may provide the molecular basis of the observed cyclic AMP effect. No such elements are present in the genes for oxytocin, the messenger ribonucleic acid levels of which were not measurably affected by forskolin and IBMX in our cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2826.1990.tb00652.x
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  • 4
    Electronic Resource
    Electronic Resource
    Structure and Expression of the Vasopressin Precursor Gene in Central Diabetes Insipidus (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 689 (1993), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1993.tb55538.x
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  • 5
    Electronic Resource
    Electronic Resource
    Single base deletion in the vasopressin gene is the cause of diabetes insipidus in Brattleboro rats (1984)
    [s.l.] : Nature Publishing Group
    Nature 308 (1984), S. 705-709 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In rats with hereditary hypothalamic diabetes insipidus (Brattleboro rats) the gene for the vasopressin precursor lacks a single G residue in the protein-coding region. The mutation gives rise to an open reading frame predicting a hormone precursor having a different ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/308705a0
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  • 6
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of cloned cDNA encoding bovine arginine vasopressin–neurophysin II precursor (1982)
    [s.l.] : Nature Publishing Group
    Nature 295 (1982), S. 299-303 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The sequence of a cDNA encoding the nonapeptide arginine vasopressin (A VP) and its carrier protein, neurophysin II (NpII) from bovine hypothalamus, proves that the 166-amino acid precursor molecule contains a signal peptide of 19 amino acids followed directly by A VP connected to NpII by a ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/295299a0
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  • 7
    Electronic Resource
    Electronic Resource
    Possible role for salivary gland protein in taste reception indicated by homology to lipophilic-ligand carrier proteins (1990)
    [s.l.] : Nature Publishing Group
    Nature 343 (1990), S. 366-369 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Cell-free translation of poly(A)+ RNA isolated from adult male rat von Ebner's glands, including the circumvallate papilla, yielded an abundant protein product with a relative molecular mass of 20,000 (Mr-20K; Fig. 1, lane 2). About 40% of the clones isolated from a complementary DNA library ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/343366a0
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  • 8
    Electronic Resource
    Electronic Resource
    Transcription of the opsin gene in degenerate eyes of cave-dwelling Astyanax fasciatus (Teleostei, Characidae) and of its conspecific epigean ancestor during early ontogeny (1993)
    Springer
    Cell & tissue research 273 (1993), S. 183-192 
    Details
    ISSN: 1432-0878
    Keywords: Opsin ; Retina ; Morphogenesis ; Regressive development ; Eye regression ; Blindness ; In situ hybridization ; Cave fish ; Astyanax fasciatus (Teleostei) Eye regression ; Cave animals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The early morphogenesis of the degenerate eyes of the Mexican cave fish Astyanax fasciatus and of its conspecific epigean ancestor has been studied comparatively using light- and electron-microscopical techniques; the transcription of the opsin gene has been analysed during early ontogeny in both populations by in situ hybridization. The opsin protein is an integral component of the disk membranes of the photoreceptor cells. In epigean specimens, its expression is correlated with the beginning of outer segment formation on the third day of development. Morphogenesis of the cave fish eye is similar to that of the epigean eye until end of the third day. However, eye growth and morphogenesis of the retina are delayed and sporadic cell death occurs in all retinal layers at the beginning of the second day. Retinal cytodifferentiation breaks off at the point of outer segment formation. Cave specimens are not able to develop regular outer segments at any stage, but the opsin gene is nevertheless expressed in the outer nuclear layer of the developing retina for a limited period of time. On the basis of the comparative morphology and transcriptional studies of epigean and cave specimens, it is suggested that the eye regression of cave fish is primarily the result of mutations of developmental control genes and not of structural genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304625
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  • 9
    Electronic Resource
    Electronic Resource
    Postnatal development of von Ebner's glands: accumulation of a protein of the lipocalin superfamily in taste papillae of rat tongue (1992)
    Springer
    Cell & tissue research 267 (1992), S. 313-320 
    Details
    ISSN: 1432-0878
    Keywords: Salivary glands ; Carrier proteins ; Lipocalins ; Papillae, lingual ; Perireceptor event ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Antibodies produced against rat von Ebner's gland (VEG) protein, a recently characterized member of a lipophilic ligand carrier protein family, detect this protein immunocytochemically in von Ebner's gland acini and show that it is present at high concentrations in the clefts of circumvallate and foliate papillae. During embryonic development, von Ebner's gland anlagen are innervated (as shown immunocytochemically using neuronal specific antibodies) as early as embryonic day 20, before lateral glandular outgrowth and VEG protein can be observed. Expression of the VEG protein as determined by in sity hybridization and immunocytochemistry begins at postnatal day-2 cells in differentiating and branching off from von Ebner's gland ducts, and sharply increases with further enlargement and maturation of the gland. The close temporal correlation of von Ebner's gland innervation and VEG protein expression with papilla innervation and taste-bud development suggests a functional relationship of both structures. VEG protein might control access of lipophilic sapid molecules, such as bitter substances, to the gustatory receptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00302970
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  • 10
    Electronic Resource
    Electronic Resource
    Cloning and chromosomal mapping of the human p53-related KET gene to Chromosome 3q27 and its murine homolog Ket to mouse Chromosome 16 (1998)
    Springer
    Mammalian genome 9 (1998), S. 899-902 
    Details
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. KET is a member of the newly discovered family of proteins that is related to the tumor suppressor p53. Here we describe the molecular cloning of a human cDNA of 4846 bp encoding a protein of 680 amino acids. The human KET protein shares 98% identity with the previously characterized rat homolog. The remarkably high degree of conservation lends support to the notion that KET proteins have important basic functions in development and differentiation. Using the GeneBridge 4 radiation hybrid panel, we have mapped KET to human Chromosome (Chr) 3q27. KET is located between the somatostatin gene SST (proximal) and the apolipoprotein D gene APOD (distal) in a region of conserved synteny to mouse Chr 16. This chromosomal region is deleted in early stages of tumorigenesis of mouse islet cell carcinomas and contains the hitherto unidentified Loh2 gene, a putative suppressor of angiogenesis. The murine homolog Ket was mapped in an interspecific backcross panel and falls into this region of loss of heterozygosity. From our mapping data we infer that KET might act as a tumor suppressor and is considered as a candidate for Loh2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003359900891
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