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  • 1
    Electronic Resource
    Electronic Resource
    Investigations into the Barrier(s) to Interspecific Hybridization between Cicer arietinum L. and eight other Annual Cicer species (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 100 (1988), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Barrier(s) to interspecific hybridization between the cultivated chickpea, Cicer arietinum L., and eight other annual wild species, i.e. C. reticulatum Lad., C. echinospermum Dav., C. pinnatifidum J. and S., C. judaicum Boiss., C. bijugum Rech., C. chorassanicum (Bge) M. Pop., C. yamashitae Kit. and C. cuneatum Rich., were investigated. In general, good pollen germination and pollen tube growth were observed in all eight crosses and their reciprocals. En spite of a few pollen cube growth abnormalities in most crosses, pollen tube penetration into the ovule and, thus, fertilization was observed in all cross combinations. However, differences were observed in the time from pollination to fertilization, not only between different interspecific crosses but also between reciprocals of a particular interspecific cross. The crossability barrier is, therefore, believed to be due to factor(s) operating after fertilization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1988.tb00240.x
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  • 2
    Electronic Resource
    Electronic Resource
    Radar Observation of Heavy Rain (1949)
    [s.l.] : Nature Publishing Group
    Nature 163 (1949), S. 372-372 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] WHILE .^certain amount of data on radar obsecrations of tropical rain has been published, the accompanying photographs of a radar P.P.I. scree^showing a belt of heavy rain passing over the Manchester district may be of interest. The wave-Idngth used was 3 cm. and the pulse length 1 microsecond, the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/163372a0
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  • 3
    Electronic Resource
    Electronic Resource
    Development of an efficient Agrobacterium-mediated transformation system for Brassica carinata (1998)
    Springer
    Plant cell reports 17 (1998), S. 183-188 
    Details
    ISSN: 1432-203X
    Keywords: Key words Brassica ; Mustard ; Transgenic ; Transformation ; Regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot organogenesis and plant regeneration were readily achieved from cotyledonary petioles and hypocotyls of Brassica carinata. These explants were used for Agrobacterium-mediated transformation. A construct containing the selectable marker genes, neomycin phosphotransferase II, phosphinothricin acetyl transferase and the reporter gene β-glucuronidase, under the control of a tandem 35S promoter, was used for transformation. Although transformation was achieved with both cotyledonary petioles and hypocotyls, cotyledonary petioles responded best, with 30–50% of the explants producing GUS-positive shoots after selection on 25 mg/l kanamycin. Direct selection on L-phosphinothricin also produced resistant shoots but at a lower frequency (1–2%).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050375
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  • 4
    Electronic Resource
    Electronic Resource
    The development of oat microsatellite markers and their use in identifying relationships among Avena species and oat cultivars (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 1259-1268 
    Details
    ISSN: 1432-2242
    Keywords: Key words Enriched-library ; SSR ; Repetitive elements ; Allelic diversity ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Microsatellites have many desirable marker properties. There has been no report of the development and utilization of microsatellite markers in oat. The objectives of the present study were to construct oat microsatellite-enriched libraries, to isolate microsatellite sequences and evaluate their level of polymorphism in Avena species and oat cultivars. One hundred clones were isolated and sequenced from three oat microsatellite-libraries enriched for either (AC/TG) n , (AG/TC) n or (AAG/TTC) n repeats. Seventy eight clones contained microsatellites. A database search showed that 42% of the microsatellite flanking sequences shared significant homology with various repetitive elements. Alu and retrotransposon sequences were the two largest groups associated with the microsatellites. Forty four primer sets were used to amplify the DNA from 12 Avena species and 20 Avena sativa cultivars. Sixty two percent of the primers revealed polymorphism among the Avena species, but only 36% among the cultivars. In the cultivars, the microsatellites associated with repetitive elements were less polymorphic than those not associated with repetitive elements. Only 25% of the microsatellites associated with repetitive elements were polymorphic, while 46% of the microsatellites not associated with repetitive elements showed polymorphism in the cultivars. An average of four alleles with a polymorphism information content (PIC) of 0.57 per primer set was detected among the Avena species, and 3.8 alleles with a PIC of 0.55 among the cultivars. In addition, 54 barley microsatellite primers were tested in Avena species and 26% of the primers amplified microsatellites from oat. Using microsatellite polymorphisms, dendrograms were constructed showing phylogenetic relationships among Avena species and genetic relationships among oat cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051605
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  • 5
    Electronic Resource
    Electronic Resource
    The genetic basis of scald resistance in western Canadian barley cultivars (1995)
    Springer
    Euphytica 92 (1995), S. 367-374 
    Details
    ISSN: 1573-5060
    Keywords: marker-assisted selection ; genetics ; barley ; Hordeum vulgare ; scald ; Rhynchosporium secalis ; Canada
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The genetic basis of resistance to scald (Rhynchosporium secalis) within barley breeding populations is poorly understood. The design of effective genetically based resistance strategies is predicated on knowledge of the identity of the resistance genes carried by potential parents. The resistance exhibited by a broad selection of western Canadian barley lines was investigated by evaluating their reactions to five R. secalis isolates. Results were compared to the resistance exhibited by previously characterized lines. This comparison, combined with pedigree analysis indicated that there are two different resistance genes present inwwestern Canadian cultivars. These genes were shown to be independent through analysis of a segregating population derived from a cross between Falcon and CDC Silky. This evidence, along with observed linkage of the gene in CDC Silky with an allele specific amplicon developed for a Rhynchosporium secalis resistance locus on chromosome 3, provides evidence that the gene in Falcon is the Rh2 gene derived from Atlas, and the gene (s) in CDC Silky is located within the Rh/Rh3/Rh4 cluster and is similar to the Rh gene in Hudson.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037121
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  • 6
    Electronic Resource
    Electronic Resource
    Variation in the ribosomal RNA units of the generaLens andCicer (1995)
    Springer
    Euphytica 83 (1995), S. 33-42 
    Details
    ISSN: 1573-5060
    Keywords: Lens ; Cicer ; 5S rRNA ; 18-25S rRNA ; restriction map ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Investigation of the organization of the tandemly repeated 5S and 18–25S ribosomal RNA (rRNA) genes was carried out on members of the generaLens andCicer using restriction endonuclease digestion and Southern hybridization. The 5S rRNA unit (gene+spacer) inLens (0.38 to 0.50 kb) andCicer 0.50 to 0.44 kb) is the largest among the legumes. InL. nigricans andL. culinaris ssp.odemensis a second repeat size was detected. Restriction maps for the 18–25S units ofLens andCicer species were developed. The 18–25S rRNA unit varied from 8.3 to 9.8 kb inLens and 10.5 to 11.4 kb inCicer. The only detectable variability in theLens andCicer 18–25S rRNA unit was in the length of the intergenic spacer (IGS) region, except for a HindIII site in the IGS region ofL. nigricans ssp.nigricans and in allCicer species. Similarities in the size of both 5S and 18–25S rRNA units were noted betweenL. culinaris ssp.culinaris andL. culinaris ssp.orientalis, which supports previous evidence thatL. culinaris ssp.orientalis is the progenitor of the cultivated lentil. Differences in the arrangement of both 5S and 18–25S rRNA units were noted betweenL. nigricans ssp.nigricans andL. nigricans ssp.ervoides, indicating either divergent evolution of these subspecies or alternatively incorrect taxonomy. On the basis of the size of the 5S rRNA unit,C. bijugum, C. chorassanicum andC. echinospermum formed a group with a slightly smaller unit than the otherCicer species. Similarities in the length of both 5S and 18–25S rRNA units ofC. arietinum andC. reticulatum possibly confirmC. reticulatum as the progenitor ofC. arietinum. Cicer cuneatum has a smaller 18–25S rRNA unit than the otherCicer species as a result of a smaller intergenic spacer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01677858
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  • 7
    Electronic Resource
    Electronic Resource
    Determination of genetic variation and taxonomy in lentil (Lens Miller) species by chloroplast DNA polymorphism (1991)
    Springer
    Euphytica 56 (1991), S. 213-218 
    Details
    ISSN: 1573-5060
    Keywords: chloroplast DNA ; Lens ; polymorphism ; restriction fragments ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Chloroplast DNA restriction fragment lenght polymorphisms (RFLP) were used to examine the taxonomic relationships of cultivated and wild lentil (Lens Miller) species and identify the extent of genetic variation in this genus. Twelve accessions representing all Lens subspecies were digested with four hexanucleotide-recognizing restriction endonucleases. These digests randomly surveyed 540 base pairs, or 0.4% of the approximately 125 kilobase lentil chloroplast genome. A high degree of gragment length conservation was seen among members of crossability group I, i.e., L. c. ssp. culinaris, L. c. ssp. orientalis and L. c. ssp. odemensis. Accessions of the two subspecies comprising crossability group II, i.e., L. n. ssp. nigricans and L. n. ssp. ervoides, showed the greatest amount of variation when compared to the cultivated lentil, L. c. ssp. culinaris. Limited variation was observed within subspecies except for L. n. ssp. nigricans, where accessions of the normal cytotype were highly polymorphic to those of the differentiated cytotype. Chloroplast DNA RFLPs reaffirm hypotheses that propose L. c. ssp. orientalis as the progenitor to the cultivated lentil. The implications of this study on taxonomy and genetic resources is also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042366
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  • 8
    Electronic Resource
    Electronic Resource
    Frequent duplication and deletion events in the 5S RNA genes and the associated spacer regions of theTriticeae (1988)
    Springer
    Plant systematics and evolution 160 (1988), S. 105-122 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticeae ; 5 S DNA sequence analyses ; duplication and deletion events ; phenetic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The 5 S DNA units from 15 grasses in theTriticeae were analysed at the DNA sequence level. Four units carried duplications near the 3′-end of the 5 S RNA gene with 3 of the duplications centred on the same base pairs as a duplication previously reported byGerlach & Dyer. The fourth duplication was located 3′ downstream from the gene, in the spacer region. Apparent deletions were very frequent when units of the different grasses were compared and it was clear that these deletions did not extend into a 75 bp spacer region upstream from the 5 S RNA gene. This 75 bp region also tended to be more conserved between the grasses as compared to the high level of sequence change in the rest of the spacer region. — Phenetic relationships were established between the grasses using the sequence data. The relationships were generally consistent with the data from other parameters and, in addition, showed that two Australian grasses were closely related to the other Northern hemisphere genera examined. The data concerning the Australian grasses is discussed in relation to the isolated nature of Australia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00936713
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