ISSN:
1617-4623
Keywords:
Xanthomonas
;
Tn5 mutagenesis
;
Nonpathogenic mutant
;
Pathogenicity genes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Mutants of a tomato strain ofXanthomonas campestris pv.vesicatoria (XCV), causal agent of bacterial spot of tomato and pepper, were produced using the transposon Tn5 carried in the suicide plasmid pGS9. One prototrophic mutant, M461, was isolated which caused no visible reaction on tomato or pepper, but maintained the wild-type ability to induce a hypersensitive reaction (HR) on tobacco. This mutant showed similar growth characteristics to the wild-type in culture, but growth in planta was reduced. A genomic library of wild-type XCV was constructed in the broad host range cosmid vector pLAFR3. Clone p6AD4 restored pathogenicity to M461 on tomato and the ability to induce a HR on pepper. This clone contained ca. 22 kb of XCV DNA. The insertion in M461 was in a site corresponding to a 1.1 kbEcoRI fragment of p6AD4.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00633855
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