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  • 1
    Electronic Resource
    Electronic Resource
    Antiserum against Xanthomonas phytopathogen inhibits host—Pathogen interaction in seedlings of Brassica campestris (1987)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 44 (1987), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Xanthomonas campestris pv campestris (X.c.c.), the causal agent of crucifer black rot, causes turnip seedlings to collapse and rot. X.c. pv vitians (X.c.v.) is a non-pathogen of turnips and triggers a resistance response. Transconjugants of X.c.c., containing defined fragments of DNA from X.c.v., which have reduced pathogenicity to turnips were constructed. A polyclonal antiserum raised against X.c.v. restored the pathogenicity of one of these transconjugants. Antiserum ‘purged’ by reaction with sonicates of X.c.c. was equally effective in restoring pathogenicity. The purged antiserum recognised considerably fewer components than the original antiserum following Western blotting of bi-dimensional gels. This subset of antigens appeared to be localised primarily on the bacterial cell surface. The results indicate the potential use of purged polyclonal antisera in identifying bacterial determinants that trigger host resistance responses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1987.tb02318.x
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  • 2
    Electronic Resource
    Electronic Resource
    A two-component system involving an HD-GYP domain protein links cell–cell signalling to pathogenicity gene expression in Xanthomonas campestris (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 38 (2000), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The synthesis of extracellular enzymes and extracellular polysaccharide (EPS) in Xanthomonas campestris pv. campestris (Xcc) is regulated by a cluster of genes called rpf (for regulation of pathogenicity factors). Two of the genes, rpfF and rpfB, have previously been implicated in the synthesis of a diffusible regulatory molecule, DSF. Here, we describe a screen of transposon insertion mutants of Xcc that identified two DSF-overproducing strains. In each mutant, the gene disrupted is rpfC, which encodes a hybrid two-component regulatory protein in which the sensor and regulator domains are fused and which contains an additional C-terminal phosphorelay (HPt) domain. We show that rpfC is in an operon with rpfH and rpfG. The predicted protein RpfG has a regulatory input domain attached to a specialized version of an HD domain, previously suggested to function in signal transduction. The predicted protein RpfH is structurally related to the sensory input domain of RpfC. We show that RpfC and RpfG act positively to regulate the synthesis of extracellular enzymes and EPS, but that RpfC acts negatively to regulate the synthesis of DSF. We propose that RpfGHC is a signal transduction system that couples the synthesis of pathogenicity factors to sensing of environmental signals that may include DSF itself.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2000.02196.x
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  • 3
    Electronic Resource
    Electronic Resource
    Erratum to “16S rRNA gene sequence analyses and inter- and intrageneric relationships of Xanthomonas species and Stenotrophomonas maltophilia” (1997)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 155 (1997), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1997.tb13883.x
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  • 4
    Electronic Resource
    Electronic Resource
    Rotational population distributions of O2(X 3Σg−, v‘=9, 12, and 15) following ozone photolysis at 248 nm (1993)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 98 (1993), S. 321-330 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Vibrationally excited O2(X 3Σg−, v‘=9, 12, and 15) produced from the 248 nm photolysis of O3 was probed using laser-induced fluorescence of the (0,v‘) Schumann–Runge bands of O2(B 3Σu−←X 3Σg−). The nascent rotational distributions are all sharply peaked at 15% of the available kinetic energy. Comparison to statistical and impulsive models of photodissociation suggests that the energy release is largely impulsive. However, while the impulsive model accurately predicts the rotational energy distributions of O2(X 3Σg−), it does not simultaneously match previously published photoproduct angular distributions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.464625
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  • 5
    Electronic Resource
    Electronic Resource
    A novel proline-rich glycoprotein associated with the extracellular matrix of vascular bundles of Brassica petioles (1997)
    Springer
    Planta 202 (1997), S. 28-35 
    Details
    ISSN: 1432-2048
    Keywords: Key words:Brassica (glycoprotein) ; Extracellular matrix glycoprotein (immunolocalisation) ; Petiole (glycoprotein) ; Proline-rich glycoprotein ; Vascular bundle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. A panel of monoclonal antibodies (MAC204, MAC236, MAC265) which recognise extracellular matrix glycoproteins implicated in plant-microbe interactions has been used to study glycoprotein antigens in petioles of turnip (Brassica campestris L.). While MAC204 recognised two glycoproteins (gp120 and gp45) with apparent Mr 120 000 and 45 000 in petiole extracts made with 2-amino-2-(hydroxymethyl)-1,3-propanediol (Tris) buffer containing sodium dodecyl sulfate, MAC236 recognised gp120 but not gp45, and MAC265 gave no or only weak reactivity. Tissue dissection studies established that gp120 was predominantly associated with the vascular bundle whereas gp45 was largely associated with the pith. This was consistent with results from tissue prints probed with MAC204 and MAC236 which also suggested a vascular localisation for gp120. Immunoelectronmicroscopy showed that MAC204 and MAC236 both labelled three-way junctions between cells of the phloem and sclerid fibres. Both gp120 and gp45 were shown to carry epitopes in common with known hy`droxyproline-rich glycoproteins. Unlike gp45, gp120 could be extracted from petioles with Tris buffer alone and then isolated from this extract by trichloroacetic acid treatment (which left gp120 soluble), followed by size-exclusion and ion-exchange chromatography. Amino acid analysis revealed gp120 to be a novel glycoprotein, particularly rich in proline, lysine, valine and threonine but relatively poor in hydroxyproline. The most abundant sugars were arabinose and galactose. The potential role of this very basic cell surface glycoprotein in plant defence against microbes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050099
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  • 6
    Electronic Resource
    Electronic Resource
    Gene expression in Brassica campestris showing a hypersensitive response to the incompatible pathogen Xanthomonas campestris pv. vitians (1987)
    Springer
    Plant molecular biology 8 (1987), S. 405-414 
    Details
    ISSN: 1573-5028
    Keywords: hypersensitive response ; Brassica campestris ; Xanthomonas campestris pv. vitians ; mRNA induction ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Xanthomonas campestris pv. vitians, a pathogen of lettuce, elicits a hypersensitive response within 12 hours of inoculation into Brassica leaves, characterized by tissue collapse, loss of membrane integrity, vein blockage and melanin production. In contrast, the compatible pathogen, X. c. pv. campestris, has no visible effects on leaves for 48 hours, after which inoculated areas show chlorosis which eventually spreads, followed by rotting. mRNA was prepared from leaves inoculated with suspensions of both pathovars or with sterile medium up to 24 hours following inoculation. In vitro translation of total and poly A+ RNA in rabbit reticulocyte lysate in the presence of 35S methionine followed by separation of the polypeptide products by 2D-PAGE, allowed comparison of the effects of these treatments on plant gene expression. Major changes in gene expression were observed as a consequence of the inoculation technique. In addition, after inoculation with X. c. vitians, up to fifteen additional major polypeptides appeared or greatly increased by four hours. Some of these had disappeared by nine hours and several more had appeared. No major polypeptides disappeared or decreased greatly in intensity following inoculation with X. c. vitians.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015818
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  • 7
    Electronic Resource
    Electronic Resource
    Use of oligonucleotide probes to identify members of two-component regulatory systems in Xanthomonas campestris pathovar campestris (1990)
    Springer
    Molecular genetics and genomics 222 (1990), S. 145-151 
    Details
    ISSN: 1617-4623
    Keywords: Xanthomonas ; Xanthan ; Two-component regulatory systems ; Oligonucleotide probing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two-component regulatory systems comprising a sensor and a regulator protein, both with highly conserved amino acid domains, and commonly genetically linked, have been described in a range of bacterial species and are involved in sensing environmental stimuli. We used two oligonucleotide probes matching the postulated coding regions for domains of sensor and regulator proteins respectively in Xanthomonas campestris pathovar campestris (Xcc) to identify possible two-component regulatory systems in Xcc. Two different fragments of Xcc DNA with homology to both of these probes were cloned. The DNA sequence of part of one of these fragments encompassed a potential open reading frame (ORF), the predicted amino acid sequence of which had extensive homology with regulator proteins of two-component regulatory systems. Analysis of the predicted amino acid sequence for the 3′ end of an adjacent ORF revealed a very high level of homology with the C-terminal end of sensor proteins. Strains of Xcc with Tn5-induced mutations in the regulator gene were affected in extracellular polysaccharide production, and also in resistance to salt and chloramphenicol. No effects of mutation in the second clone were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00283036
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  • 8
    Electronic Resource
    Electronic Resource
    Cloning of genes involved in negative regulation of production of extracellular enzymes and polysaccharide of Xanthomonas campestris pathovar campestris (1990)
    Springer
    Molecular genetics and genomics 222 (1990), S. 157-160 
    Details
    ISSN: 1617-4623
    Keywords: Amylase ; Endoglucanase ; Protease ; Polygalacturonate lyase ; Pathogenicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A recombinant plasmid pIJ3079 contains DNA sequences from Xanthomonas campestris pv campestris involved in coordinate negative regulation of production of the extracellular enzymes protease, endoglucanase, amylase and polygalacturonate lyase, and extracellular polysaccharide (EPS). Wild-type bacteria harbouring pIJ3079 and therefore carrying extra copies of the gene(s) therein showed reduced enzyme and EPS production and reduced aggresiveness to plants. Localised Tn5 mutagenesis of the corresponding region of the genome gave mutants producing higher levels of enzymes and EPS than the wild type, suggesting that the gene(s) may negatively regulate production in the normal cell. Enzyme and EPS production in the mutants was still dependent on previously characterised positive regulatory genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00283038
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  • 9
    Electronic Resource
    Electronic Resource
    A multipurpose broad host range cloning vector and its use to characterise an extracellular protease gene of Xanthomonas campestris pathovar campestris (1990)
    Springer
    Molecular genetics and genomics 220 (1990), S. 433-440 
    Details
    ISSN: 1617-4623
    Keywords: Deletion ; DNA sequencing ; Subtilisin ; Serine protease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A multipurpose broad host range plasmid, pIJ3200, was constructed by inserting the polylinker-containing 445 by PvuII fragment of Bluescript M13 into the EcoRI site of the cosmid pLAFR1. Using this vector a protease gene of Xanthomonas campestris pathovar campestris, previously cloned in the recombinant plasmid pIJ3070, was located by deletion to a 2.2 kb DNA region. Sequencing of the protease gene revealed an open reading frame encoding a 580 amino acid polypeptide with molecular weight of 57000. The deduced amino acid sequence showed strong homology with the subtilisin family of serine proteases. This, together with its sensitivity to inhibition by phenylmethylsulphonyl fluoride, suggests that the enzyme belongs to this family of proteases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391750
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  • 10
    Electronic Resource
    Electronic Resource
    Structural characterization of protein secretion genes of the bacterial phytopathogen Xanthomonas campestris pathovar campestris: relatedness to secretion systems of other gram-negative bacteria (1991)
    Springer
    Molecular genetics and genomics 229 (1991), S. 357-364 
    Details
    ISSN: 1617-4623
    Keywords: Protein export ; Pathogenicity ; DNA sequence ; Xanthomonas campestris pv. campestris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleotide sequence was determined of a 5.3 kb region of the Xanthomonas campestris pathovar campestris genome carrying a gene cluster encoding protein secretion and pathogenicity functions. A putative promoter sequence and five open reading frames (ORF) which may be part of an operon were revealed. The five predicted primary translation products comprise 531, 390, 147, 169 and 138 amino acids with Mr values of 58854, 42299, 15548, 18214 and 15108 respectively. A sixth, partial ORF is also present. Between ORF1 and ORF2 is a sequence of unknown function showing 7 by duplications. The deduced amino acid sequence of ORF1 is related to the Klebsiella pneumoniae PulE protein, to the Bacillus subtilis ComG ORF1 and to the Agrobacterium tumefaciens VirB ORF11 products. In addition, the deduced amino acid sequence of ORF2 showed homology to the Pu1F and to the ComG ORF2 products. The proteins encoded by ORF3, 4 and 5 showed amino acid homology to PulG, H and I products respectively. The proteins encoded by ORF2, 3, 4 and 5 showed significant hydrophobic domains which may represent membrane-spanning regions. By contrast the protein encoded by ORF1 was largely hydrophilic and had two putative nucleoside triphosphate binding sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267456
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