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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of Lolium temulentum geographical differentiation by microsatellite and AFLP markers (2005)
    Oxford, UK : Blackwell Science Ltd
    Weed research 45 (2005), S. 0 
    Details
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The genetic diversity and relationships of 48 Lolium temulentum accessions derived from eight countries (Morocco, Egypt, Tunisia, Italy, Ethiopia, Pakistan, Nepal and Japan) were analysed using seven microsatellite and 44 AFLP polymorphic loci to investigate the origin and distribution of genetic variation. Cluster analysis was performed using the unweighted pair-group method with arithmetic averages (UPGMA) based on the simple matching coefficient of similarity. Nei's gene diversity (h) and the coefficient of gene differentiation (Gst) were calculated. The results from microsatellite analysis indicated that accessions from the same country did not always cluster together, probably because of the limited number of loci used. In contrast, AFLP clearly separated clusters between countries and/or regions; Pakistan–Nepal complex, the Mediterranean region, Ethiopia and Japan. The h value was much higher for microsatellite than for AFLP analysis, indicating that microsatellites are the more variable markers. For AFLP analyses, h values were highest in accessions from Pakistan–Nepal complex, and from the Mediterranean region. These are in agreement with proposals that the origin of L. temulentum lies between the South-western Asia and the Mediterranean basin. The clear groupings of accessions from each country and/or region with high Gst (0.688) indicate that exchange of seeds between them is limited.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3180.2004.00420.x
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  • 2
    Electronic Resource
    Electronic Resource
    Development of microsatellite markers and their effectiveness in Lolium temulentum (2004)
    Oxford, UK : Blackwell Science Ltd
    Weed research 44 (2004), S. 0 
    Details
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Screening a genomic library of Lolium temulentum (darnel) with synthetic (GT)15 and (GA)15 oligonucleotides, we identified microsatellite-containing clones. Based on the sequence data of the clones, specific primer pairs were synthesized and their effectiveness examined using 10 L. temulentum and two L. persicum accessions from Asia, Africa and Europe. Fifteen primer pairs amplified single fragments, and five of them showed polymorphism in polyacrylamide gel electrophoresis in the L. temulentum and L. persicum accessions. Fifteen primer pairs reported in L. perenne (perennial ryegrass) were also applied in this study. Nine of them amplified the microsatellite alleles, and two of the nine showed polymorphic alleles in L. temulentum and L. persicum. For the polymorphic markers, polymorphic information content values ranged from 0.60 to 0.86 with an average of 0.76 among 10 L. temulentum accessions. The microsatellite markers developed for L. temulentum and L. perenne should be informative and powerful in analysing the intraspecific genetic diversity and the phylogenetic relationships of L. temulentum and L. persicum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3180.2004.00382.x
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  • 3
    Electronic Resource
    Electronic Resource
    Crystal growth of ribonuclease S under microgravity
    Amsterdam : Elsevier
    Journal of Crystal Growth 122 (1992), S. 323-329 
    Details
    ISSN: 0022-0248
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0022-0248(92)90264-J
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  • 4
    Electronic Resource
    Electronic Resource
    Crystal growth of ribonuclease S under microgravity
    Amsterdam : Elsevier
    Journal of Crystal Growth 122 (1992), S. 323-329 
    Details
    ISSN: 0022-0248
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0022-0248(92)90264-J
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  • 5
    Electronic Resource
    Electronic Resource
    Relationship between estrogen receptor (ER) and human gastric cancer
    Amsterdam : Elsevier
    Journal of Steroid Biochemistry 28 (1987), S. 36 
    Details
    ISSN: 0022-4731
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0022-4731(87)91204-0
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  • 6
    Electronic Resource
    Electronic Resource
    Plastic Deformation in the Sliding Wear Surfaces of Alumina at Various Temperatures (1998)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 161-163 (July 1998), p. 603-606 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/45/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.161-163.603.pdf
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  • 7
    Electronic Resource
    Electronic Resource
    Expression and localization of the LGN protein in the mouse brain with reference to its relationship with Gαi2 (1999)
    Springer
    Medical electron microscopy 32 (1999), S. 221-225 
    Details
    ISSN: 1437-773X
    Keywords: Key words LGN ; Gαi2 ; Mouse brain ; Immunoblotting ; Immunofluorescence microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract It has been suggested that the LGN protein is associated with Gαi2 by the yeast two-hybrid system and in vitro pull-down assay. To determine the functions of LGN in the central nervous system, we examined the expression and localization of LGN in mouse brain by immunoblotting and immunofluorescence microscopy. By immunoblotting, almost similar amounts of LGN were detected in the olfactory bulb, cerebral cortex, hippocampus, and cerebellum of the adult mouse brain, and the levels of the postnatal LGN expression in the whole brain were fairly constant. Immunofluorescence microscopy showed that LGN is localized in nuclei of the neurons in the olfactory bulb, cerebral cortex, and hippocampus, but in both nuclei and cytoplasm of Purkinje cells in the cerebellum. On the other hand, Gαi2 was distributed throughout the neuronal elements except for the nuclei. Thus, LGN and Gαi2 were colocalized in the cytoplasm of Purkinje cells, but not in other neurons examined. These results suggest that LGN may be involved not only in the Gαi2-mediated signaling but also in other signaling pathways.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007950050006
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  • 8
    Electronic Resource
    Electronic Resource
    Localization of a novel GAP family protein SPAL in the rat esophagus and heart (1999)
    Springer
    Medical electron microscopy 32 (1999), S. 20-24 
    Details
    ISSN: 1437-773X
    Keywords: Key words SPAL ; β-catenin ; ZO-1 ; Esophagus ; Heart
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Spa-1-like protein (SPAL) is a novel GTPase-activating protein (GAP) family protein. We generated anti-SPAL antibody, and examined localization of SPAL in the rat esophagus and heart by immunofluorescence microscopy. In the esophagus, SPAL was highly expressed in the spinous and granular layers and localized at the cell–cell border of the epithelial cells. SPAL in the spinous layer was almost colocalized with β-catenin, an intracellular anchor protein of E-cadherin, while colocalization of SPAL with the tight junction protein ZO-1 was shown in the granular layer. In the heart, SPAL was localized at the intercalated disks, and there colocalized with both β-catenin and ZO-1. In addition, much punctate localization of SPAL was seen along lateral borders of the cardiomyocytes, where β-catenin and ZO-1 were absent. From these results, it is suggested that SPAL may regulate cell–cell adhesion in the rat esophagus and heart.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007950050004
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of alignment of the transplanted graft extracellular matrix on cellular repopulation and newly synthesized collagen (1998)
    Springer
    Archives of orthopaedic and trauma surgery 117 (1998), S. 215-221 
    Details
    ISSN: 1434-3916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study examines the effect of alignment of the transplanted graft extracellular matrix on cellular repopulation and new collagen synthesis. The lateral half of the patellar tendon was harvested as a tendon graft from Lewis rats and frozen at –80° C. In order to maintain the original alignment of the graft extracellular matrix, the graft was transplanted to a same size defect in the patellar tendon of other Lewis rats (group I). For controls, the graft was transplanted in a lax condition after excision of only the distal half of the lateral side of a patellar tendon (group II). After transplantation, six animals in each group were killed at 3, 7, 14, and 28 days. Cellular repopulation was assessed by using fibrillar-actin (F-actin) labeling with rhodamine-phalloidin, and new collagen synthesis was detected by means of a polyclonal antibody against type III collagen aminopropeptide (pN collagen III). Collagen fibril profiles were observed under the transmission electron microscope. On the 3rd day after transplantation, no specific fluorescence was detected in either group. Specific labeling for F-actin and pN collagen III, however, was observed at both ends of the graft in both groups at 1 week and throughout the graft at 2 weeks after transplantation. Consistent with the actin bundles’ orientation, pN collagen III was aligned parallel to the longitudinal axis of the graft in group I. Collagen fibrils with a smaller diameter, mixed evenly and everywhere with a larger diameter, increased gradually in group I. However, smaller collagen fibrils in group II increased more slowly and were distributed unevenly. In summary, the dense collagen arrangement in the native patellar tendon determined the alignment of the repopulating cells, and the distribution of newly synthesized collagen might be affected by the actin cytoskeleton within the repopulating cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004020050232
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  • 10
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of calspectin (a non-erythroid spectrin-like protein) in thyroid glands of normal and TSH-treated rats (1987)
    Springer
    Histochemistry and cell biology 86 (1987), S. 537-539 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization of calspectin (fodrin, a nonerythroid spectrin-like protein), which is known to bind calmodulin and F-actin, was detected in the thyroid gland of normal and TSH-treated rats by means of light-microscopic immunocytochemistry. Calspectin was demonstrated in the cytoplasm of the follicle epithelial cells especially along the baso-lateral plasma membrane in normal rats. In TSH-treated animals, in addition to the baso-lateral plasma membrane region, the apical plasma membrane region of the follicle epithelial cells also showed positive reaction to the immunostaining. These results suggest that calspectin, in conjugation with calmodulin and actin, play a role in the secretory activities including reabsorption activity of colloid of the follicle epithelial cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00489544
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