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  • 1
    Electronic Resource
    Electronic Resource
    The use of fed batch cultivation for achieving high cell densities in the production of a recombinant protein in Escherichia coli (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 14 (1994), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: The production of the fusion protein staphylococcal protein A/E. coliβ-galactosidase in Escherichiacoli was studied in batch and fed batch cultivations. Batch cultivation of a recombinant E. coli strain yielded a final cell dry weight of 16.4 g 1-1 with a final intracellular product concentration of recombinant protein corresponding to approximately 38% of the cell dry weight. Fed batch cultivation made it possible to increase the final cell dry weight to 77.0 g 1-1. The intracellular product concentration (25%) was lower as compared to batch cultivation resulting in a total concentration of recombinant protein of 19.2 g 1-1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.1994.tb00072.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Modeling of high cell density fed batch cultivation (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology reviews 14 (1994), S. 0 
    Details
    ISSN: 1574-6976
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: Escherichia coli was grown in carbon- and energy source-limited fed batch cultures to study the effect of osmotic stress and different feed rates on the growth kinetics. An unstructured model based on the linear equation for substrate consumption provided an adequate description of the bacterial growth during the first phase of biomass production (20 h), except for cultures exposed to osmotic stress by the addition of 0.5 M NaCl. The addition of salt to the culture media had a large effect on the energetics, that could not simply be described in terms of an increased maintenance requirement. In the later phase of growth, an extensive decline in viability for all cultures was observed. Coincidentally, the specific sugar uptake rate approached a lower limit. It is concluded that the total obtainable biomass in a fed batch culture is strongly affected by the magnitudes of the substrate feed rate and the ionic strength of the culture medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6976.1994.tb00070.x
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Batch and fed batch cultivations for the temperature induced production of a recombinant protein inEscherichia coli (1991)
    Springer
    Biotechnology letters 13 (1991), S. 609-614 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The temperature induced intracellular production of the fused protein SpA-βgal (protein A/β-galactosidase) withE. coli was compared in batch and fed batch culture. By introducing fed batch cultivation a final cell dry weight of 77.0 g/l was achieved, as compared to 16.4 g/l in batch cultivation. The concentration of SpA-βgal in the fed batch cultivation was very high, 19.2 g/l. This corresponded to 25% of the cell dry weight.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01033419
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    Paper (German National Licenses)
    Fulltext
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