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Fermentation ofCandida utilis for uricase production (1990)

Adámek, Vladimír ; Suchová, Martina ; Demnerová, Kateřina ; [et al.]

Springer

Journal of industrial microbiology and biotechnology 6 (1990), S. 85-89

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ISSN: 1476-5535

Keywords: Uricase production ; Candida utilis ; Inducer of uricase production ; Fermentation kinetics

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Summary Conditions for the production of microbial uricase byCandida utilis were studied. For the selected strain, hypoxanthine proved to be the most effective inducer of uricase formation. The highest values of biomass as well as uricase activity in the mechanically agitated fermentor were obtained under the following conditions: 50 h, rotation impeller speed 7 s−1, air flow rate 1.25×10−5 m3s−1, concentration of inducer 0.1%.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01576427

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Study of cell-free protein synthesis in aqueous two-phase systems (1995)

Marszal, Ewa ; Suchova, Martina ; Konecny, Premysl ; [et al.]

Chicester [u.a.] : Wiley-Blackwell

Journal of Molecular Recognition 8 (1995), S. 151-156

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ISSN: 0952-3499

Keywords: cell-free translation ; phase partitioning ; protein synthesis ; dextran-PEG two phase system ; rabbit reticulocyte lysate ; wheat germ extract ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine

Notes: Protein synthesis in an aqueous two-phase system is reported here as a novel type of extractive bioconversion. Translation of BMV RNA was studied using either rabbit reticulocyte lysate or wheat-germ extract in aqueous dextran-PEG systems. Both polymers inhibited protein synthesis and the translation system partitioned into both phases. In the rabbit reticulocyte two-phase system, protein synthesis reached 30% of that in free solution, while in wheat in wheat-germ extract it was 60% of that in free solution. Protein was found mainly in the dextran (lower) phase but its partitioning was related to the polymer concentration, and molecular weight, as well as the ionic strength of the system. Protein synthesis was highly influenced by PEG concentration, potassium chloride concentration, and dextran molecular weight.

Additional Material: 6 Ill.