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  • 1
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The activities of the catecholamine synthetic enzymes tyrosine hydroxylase and phenylethanolamine N-methyltransferase, and the concentrations of the catecholamines and their respective metabolites, have been measured in the dorsal and ventral halves of the brainstem at various ages in the embryonic and adult rat. The activity of phenylethanolamine N-methyltransferase in both parts of the brainstem at day 14 of gestation is at or greater than adult levels and thereafter displays relatively small variations during ontogeny. Tyrosine hydroxylase activity, in contrast, is undetectable at day 14 and increases slowly, achieving only 20–25% of adult values by day 18 of gestation. Adrenaline concentrations correlate well with the activity of phenylethanolamine N-methyltransferase, showing a precocious development, whereas noradrenaline and 3, 4-dihydroxyphenylethylamine (dopamine) concentrations are more closely related to the enhancement of tyrosine hydroxylase activity; at day 18 of gestation, for example, they are only 5 and 10%, respectively, of the adult values. The concentrations of the metabolites of noradrenaline and dopamine are suggestive of a high rate of turnover. These results confirm previous immunocytochemical evidence of a tardy appearance of tyrosine hydroxylase-like immunoreactivity in the phenylethanolamine N-methyltransferase-positive perikarya of the embryonic medulla oblongata. In addition, the abundance of adrenaline in this area at early gestational stages strongly suggests that, despite the paucity of tyrosine hydroxylase, phenylethanolamine N-methyltransferase is active in vivo and is utilizing a substrate other than noradrenaline. It is likely, however, that at later stages of gestation, when tyrosine hydroxylase is present at sufficient activity to supply noradrenaline, the conventional synthetic pathway for adrenaline formation comes into being.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0533
    Keywords: Key words Spinal cord ischemia ; Glial fibrillary ; acidic protein ; Neurofilament ; Albumin ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have characterized the evolving morphological changes in the adult rat spinal cord following photochemically induced spinal cord ischemia. In cresyl violet-stained sections, disintegration of the tissue at the epicenter was evident at 6 h. This was preceded at 1 h post ischemia by an albumin immunoreactivity. The albumin immunoreactivity was increased at 6 and even more so at 24 h post ischemia. At 72 h post ischemia the albumin immunoreactivity was decreased. The size of the lesion was established by 3 days after the onset of ischemia. During the 1st week post ischemia, neurofilament (NF) immunohistochemistry showed swollen axons adjacent to the injured tissue. From 2 weeks post ischemia an increasing number of regrowing NF-immunoreactive axons could be seen in the center of the necrotic cavity. At 3 weeks after ischemia, a developing gliosis was observed around and rostral to the lesion cavity, as evidenced by increased glial fibrillary acidic protein (GFAP) immunoreactivity. The gliosis became more pronounced until 6 weeks post ischemia, at which time enlarged GFAP-immunoreactive cells could be seen in the remaining viable tissue bordering the necrotic areas. In this study we show that several traits in the development of a spinal cord lesion after photochemically induced ischemia are similar to those described previously after traumatic spinal cord lesions.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-069X
    Keywords: Key words Serotonin ; Allergic contact dermatitis ; Immunohistochemistry ; High-performance liquid ; chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Allergic contact dermatitis (ACD) is a common clinical condition leading to considerable morbidity. We have recently demonstrated that ketanserin, a serotonin antagonist, significantly inhibits nickel sulphate-induced ACD. Furthermore, serotonin-immunoreactive (IR) cells have previously been demonstrated in normal human cutaneous melanocytes. To further elucidate the role of serotonin in cutaneous contact hypersensitivity, we compared ACD involved skin and uninvolved skin from nickel-allergic patients, and normal skin from healthy volunteers, for the presence of serotonin-like immunoreactive cells using immunohistochemistry. In addition, serotonin concentrations in ACD involved and uninvolved skin were compared by high-performance liquid chromatography (HPLC). In the skin of normal healthy volunteers, the serotonin-IR cells were situated in the basal layer of the epidermis. In uninvolved skin the cells were also situated in the basal layer, but they were more numerous and the immunofluorescence intensity was greater. In involved skin, the IR cells were fewer and they were found higher up in the epidermis. Also, the configuration of these cells was different: they showed enlarged and elongated dendrites as well as dendritic spines. The serotonin antiserum-labelled cells in ACD involved skin were also NKI-beteb positive (the latter is known as a reliable marker of melanocytes). The concentration of serotonin in involved skin was significantly higher than that in uninvolved skin in ACD patients (P 〈 0.05). Taken together, our previous and present results indicate that serotonin plays an important role in ACD. The basal epidermal serotonin-IR cells are more dendritic in ACD, and are found more superficial in the epidermis, where they might release their content of serotonin, thereby influencing the inflammatory process.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5036
    Keywords: architecture ; bare root ; basal sweep ; container ; dominant root ; Douglas fir ; root ; symmetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seedlings grown in containers often have deformed, spiralling, kinking root systems (reported especially in pine growing in colder climates like Fenno-Scandinavia). The current study examines the effect of containers on the root systems of Douglas fir planted in Ireland. A sample of sixteen 10-year-old Douglas fir trees, planted as either bare-rooted transplants (2/1) or containerized seedlings (Paperpot 610 (2/0)) on an acid brown earth site in Ireland, were excavated for root achitectural studies. In addition to thorough above-ground measurements, an assessment of basal sweep was also carried out. Root systems were systematically described and horizontal angles, cross-sectional areas (CSA), and maximum depths of all roots〉5 mm in diameter were recorded. Various biomass ratios and estimates for dominant roots were also calculated. Symmetry of the root systems, max. and min. numbers, and CSA of roots for sectors of various sizes were compared for the two plant types. The initially (when planted) bigger bare-rooted transplants were still larger than the container-planted trees after ten years. Of the total above-ground fresh weight, the container stock had allocated more to the crown and less to the stem compared to bare-roots. The difference in root weights between stock types was small. Basal sweep occurred on average in 50% of the bare-roots and 35% of the container trees. The direction of the basal sweep leaning was concentrated to the NE, which coincided with the direction of the slope and the prevailing wind direction. No effect of planting position in relation to the direction of site preparation was found for basal sweep. The size of the root system, fresh weight and total cross-sectional area was on average for all trees correlated to both DBH and shoot fresh weights. For containers separately, however, only root area was correlated to DBH and stem fresh weight. In spite of the difference in the above-ground size, there were no significant differences in root numbers and root area (CSA) between the two plant types. No difference in rooting depth between plant types was found. When splitting the root system into 120° horizontal sectors (1/3 of the root system) the highest numbers of roots were concentrated in the NW direction. The highest amount of root area tended to be concentrated along a NW-SE diagonal, with a dominance for the latter (SE). Sectors without dominant roots (expected to be the future main structural, stabilizing roots), varied in size (94–178°) but were on average wider in bare-roots and on average concentrated in the NE direction and the downhill side of the slope.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1573-6903
    Keywords: Alzheimer's disease ; β-amyloid ; excitatory amino acid receptors ; N-methyl-D-aspartate ; Ca2+ channels
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The neurotoxic fragment corresponding to residues 25-35 of the β-amyloid (Aβ) peptide [Aβ-(25-35)] has been shown to exert effects on (+)-[3H]5-methyl-10,11-dihydro-5H-dibenzo[a,d]-cyclohepten-5,10-imine maleate ([3H]MK-801) binding to the cation channel of the N-methyl-D-aspartate (NMDA) receptor. In the present study, we investigated whether the amidated and carboxylic acid C-terminated forms of Aβ-(25-35) [Aβ-(25-35-NH2) and Aβ-(25-35-COOH), respectively] exert effects on other excitatory amino acid receptor and cation channel types in rat cortical membranes. Both Aβ-(25-35-NH2) and Aβ-(25-35-COOH) gave statistically significant dose-dependent inhibitions of [3H]glutamate and [3H]glycine binding to the agonist recognition sites of the NMDA receptor. Ten μM Aβ-(25-35-NH2) and Aβ-(25-35-COOH) gave 25% and 20% inhibitions of [3H]glutamate binding and 75% and 70% inhibitions of [3H]glycine binding, respectively. Aβ-(25-35-NH2), but not Aβ-(25-35-COOH), gave a small (ca. 17% at 10 μM) statistically significant increase of [3H]amino-3-hydroxy-5-methylisoxazole-4-propionate ([3H]AMPA) binding. [3H]kainate binding was not significantly affected by either peptide. Similarly, neither peptide affected either the maximal level or EC50 value for calcium stimulation of [3H]nitrendipine binding. It is concluded that Aβ-(25-35) shows slight affinity for the agonist recognition sites of the NMDA receptor, but not for other excitatory amino acid receptor types or for L-type voltage-dependent calcium channels.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-6903
    Keywords: NMDA ; AMPA ; kainic acid ; [3H]noradrenaline ; spinal cord
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract N-methyl-D-aspartate (NMDA) stimulated release of [3H]noradrenaline (NA) from prelabelled rat spinal cord slices. The release was partially insensitive to tetrodotoxin (TTX) and was inhibited by the NMDA antagonist MK-801. Alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) also evoked release of [3H]NA, which was enhanced by blocking AMPA receptor desensitization with cyclothiazide. AMPA-evoked release was inhibited by the non-NMDA antagonist 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(f)-quinoxaline (NBQX) but was not affected by TTX. NMDA and AMPA showed synergistic effects, indicating co-existence of NMDA and AMPA receptors on noradrenergic terminals. Kainate evoked [3H]NA release only at high concentrations and the release was not potentiated by blocking kainate receptor desensitization with concanavalin A. Thus, the results indicate that there are stimulatory presynaptic NMDA and AMPA receptors on noradrenergic axon terminals in the spinal cord and that they interact synergistically to evoke release of [3H]NA.
    Type of Medium: Electronic Resource
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