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  • 1
    Electronic Resource
    Electronic Resource
    Endophytic bacteria expressing β-glucuronidase cause false positives in transformation of Dioscorea species (1992)
    Springer
    Plant cell reports 11 (1992), S. 452-456 
    Details
    ISSN: 1432-203X
    Keywords: β-Glucuronidase ; Dioscorea ; endophytic bacteria ; false positive transformants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary False positive transformants obtained during plant transformation experiments on species of the monocotyledonous genus Dioscorea (yam) are described. The false positive results were found to be due to endophytic bacteria which exist within aseptically micropropagated shoot cultures and which express β-glucuronidase (GUS). The bacteria were isolated and identified as two species of Curtobacterium. The expression of GUS in these organisms was found to be induced by a variety of glucuronide substrates. The induction of GUS activity in the bacteria can be inhibited by chloramphenicol, tetracycline, ticarcillin and sodium azide. Implications of these results for use of the gus gene in plant transformation work are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232689
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening (2006)
    [s.l.] : Nature Publishing Group
    Nature genetics 38 (2006), S. 948-952 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] A major component in the regulatory network controlling fruit ripening is likely to be the gene at the tomato Colorless non-ripening (Cnr) locus. The Cnr mutation results in colorless fruits with a substantial loss of cell-to-cell adhesion. The nature of the mutation and the identity of the Cnr ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng1841
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Stable transformation of the food yam Dioscorea alata L. by particle bombardment (1993)
    Springer
    Plant cell reports 12 (1993), S. 468-473 
    Details
    ISSN: 1432-203X
    Keywords: Dioscorea alata ; GUS ; transformation ; particle gun
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A biolistic particle gun was used to deliver genetic material into intact yam cells. Cultured suspension cells of D. alata were bombarded with microprojectiles coated with pBI221.2 DNA and histochemical assays were carried out to show transient GUS expression in bombarded cells. Stably transformed D. alata cells were recovered from cultured cells after bombardment with microprojectiles coated with pRT99gus harbouring both the nptII and uidA genes. Bombarded cells were selected on a medium containing geneticin (G418). Two months after bombardment, calli resistant to G418 were assayed for GUS expression. There was a 100% correlation between resistance to G418 and GUS expression. From these calli, four cell lines were established and GUS activity in each line was determined fluorometrically. The use of a specific GUS inhibitor showed that the GUS activity was due to the introduced uidA gene rather than to any intrinsic GUS-like activity originating from the plant. Incorporation of the introduced DNA into the plant genomic DNA was confirmed by Southern analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234714
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    Paper (German National Licenses)
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