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  • 1
    Electronic Resource
    Electronic Resource
    A method for isolation of milled-wood lignin involving solvent swelling prior to enzyme treatment (1995)
    Springer
    Wood science and technology 29 (1995), S. 295-306 
    Details
    ISSN: 1432-5225
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary A new lignin isolation method has been developed. Wood and pulp were subjected to ball milling, swelled in an organic solvent, and then treated with a cellulase. The enzyme digestion time could be shortened to 1 day with this method. The lignin obtained has been named Swelled Enzyme Lignin (SEL). Swelling and enzyme digestion conditions and their effects on lignins were investigated. The SEL's from wood could be directly washed with water, while those from pulp had to be washed with aqueous acetic acid because they were water soluble. The purification of crude SEL's was accomplished by extracting them with dioxane-water, and then precipitating and washing with ethyl ether. Lignin yields were 24–67% based on the total amount of lignin present. The characteristics of the SEL's were further investigated by gel-permeation chromatography (GPC), infrared and 13C nuclear magnetic resonance (NMR) spectroscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00202089
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Structural analyses of new tri- and tetrasaccharides produced from disaccharides by transglycosylation of purified Trichoderma viride β-glucosidase (1999)
    Springer
    Glycoconjugate journal 16 (1999), S. 415-423 
    Details
    ISSN: 1573-4986
    Keywords: Trichoderma viride ; β-glucosidase ; transglycosylation ; two-dimensional NMR spectroscopy ; oligosaccharide synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A new β-glucosidase was partially purified from Trichoderma viride cellulase. This β-glucosidase catalyzed a transglycosylation reaction of cellobiose to give β-D-Glc-(1→6)-β-D-Glc-(1→4)-D-Glc (1, yield: 18.8%) and β-D-Glc-(1→6)-β-D-Glc-(1→6)-β-D-Glc-(1→4)-D-Glc (2, 3.7%), regioselectively. Furthermore, the enzyme regioselectively converted laminaribiose and gentiobiose into β-D-Glc-(1→6)-β-D-Glc-(1→3)-D-Glc (3, 15.3%) and β-D-Glc-(1→6)-β-D-Glc-(1→6)-D-Glc (4, 20.2%), respectively. The structures (1–4) of the products were determined by 1H and 13C NMR spectroscopies. This high regio- and stereoselectively of the β-glucosidase could be applied for oligosaccharide synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007034728857
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Construction of shuttle vectors derived fromAcetobacter xylinum for cellulose-producing bacteriumAcetobacter xylinum (1992)
    Springer
    Biotechnology letters 14 (1992), S. 539-542 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Shuttle vector pUF106 was constructed by ligation ofAcetobacter xylinum plasmid pFF6 toEscherichia coli plasmid pUC18. It had unique restriction sites suitable for insertion of a foreign DNA fragment and conferred ampicillin resistance to a host. pUF106 transformed cellulose-producingA. xylinum ATCC10245 as well asE. coli JM109.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01023936
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    13C NMR study of cellulose triacetate in the solid state (1987)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Letters Edition 25 (1987), S. 121-126 
    Details
    ISSN: 0887-6258
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1987.140250306
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Mechanism of transition between cellulose I and cellulose II during mercerization (1978)
    New York : Wiley-Blackwell
    Journal of Polymer Science: Polymer Chemistry Edition 16 (1978), S. 1335-1342 
    Details
    ISSN: 0360-6376
    Keywords: Physics ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: It is suggested that the transformation from cellulose I to cellulose II during mercerization is the result of a progressive shift of the sheets of cellulose chains within the crystallites of a microfibril from the quarter-staggered relation in cellulose I to complete correspondence in cellulose II. Qualitatively, the results of such a shift would be consistent with the observed increases in lateral disorder, changes in cell dimensions, swelling, changes in infrared absorption, increases in reactivity of hydroxyl groups and especially with changes in the relative intensities of meridional, x-ray diffraction reflections of cellulose fibers. The consequences of such a shift also include variations of the dihedral angle at the glycosidic linkage to provide an opportunity for the conformational changes which have been deduced from Raman and infrared spectra. Such a shift may take place in the transformation of native celluloses with antiparallel structure as well as those with parallel polarity. It is consistent with and is able to explain examples of “memory” of previous treatment in cellulose samples.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1978.170160614
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    Paper (German National Licenses)
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