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  • 1
    Electronic Resource
    Electronic Resource
    Catchment urbanisation and increased benthic algal biomass in streams: linking mechanisms to management (2004)
    Oxford, UK : Blackwell Science Ltd
    Freshwater biology 49 (2004), S. 0 
    Details
    ISSN: 1365-2427
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 1. Urbanisation is an important cause of eutrophication in waters draining urban areas. We determined whether benthic algal biomass in small streams draining urban areas was explained primarily by small-scale factors (benthic light, substratum type and nutrient concentrations) within a stream, or by catchment-scale variables that incorporate the interacting multiple impacts of urbanisation (i.e. variables that describe urban density and the intensity of drainage or septic tank systems).2. Benthic algal biomass was assessed as chlorophyll a density (chl a) in 16 streams spanning a rural–urban gradient, with both a wide range of urban density and of piped stormwater infrastructure intensity on the eastern fringe of metropolitan Melbourne, Australia. The gradient of urban density among streams was broadly correlated with catchment imperviousness, drainage connection (proportion of impervious areas connected to streams by stormwater pipes), altitude, longitude and median phosphorus concentration. Catchment area, septic tank density, median nitrogen concentration, benthic light (photosynthetically active radiation) and substratum type were not strongly correlated with the urban gradient.3. Variation in benthic light and substratum type within streams explained a relatively small amount of variation in log chl a (3–11 and 1–13%, respectively) compared with between-site variation (39–54%).4. Median chl a was positively correlated with catchment urbanisation, with a large proportion of variance explained jointly (as determined by hierarchical partitioning) by those variables correlated with urban density. Independent of this correlation, the contributions of drainage connection and altitude to the explained variance in chl a were significant.5. The direct connection of impervious surfaces to streams by stormwater pipes is hypothesised as the main determinant of algal biomass in these streams through its effect on the supply of phosphorus, possibly in interaction with stormwater-related impacts on grazing fauna. Management of benthic algal biomass in streams of urbanised catchments is likely to be most effective through the application of stormwater management approaches that reduce drainage connection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2427.2004.01225.x
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of individual amino acids required for secretion within the haemolysin (HlyA) C-terminal targeting region (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 6 (1992), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The release of haemolysin from Escherichia coli involves direct secretion across both the inner and outer membranes. Secretion of HlyA is dependent upon a specific membrane export complex composed of HlyB, -D and possibly TolC. HlyA is targeted to the medium via the membrane translocation complex, by a novel C-terminal secretion signal. Previous studies involving deletion and fusion analyses have given contradictory results for the minimal length (20–60 residues) of this HlyA signal region and little is known of the nature of the specific residues and structural features required for function.In this study we have analysed, quantitatively, the effect upon secretion of many point mutations introduced into the HlyA C-terminus. The results indicate the presence of a minimal secretion signal domain whose proximal boundary extends to at least residue –46 and which contains at least four Individual residues essential for maximal secretion levels. We propose that such residues act co-operatively, forming multiple contact points with the translocator proteins, with the ‘best fit’ promoting maximal levels of secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1992.tb00868.x
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  • 3
    Electronic Resource
    Electronic Resource
    Coordination compounds of p-nitrobenzeneazotyrosine (1963)
    New York : Wiley-Blackwell
    Journal of Polymer Science Part A: General Papers 1 (1963), S. 3449-3458 
    Details
    ISSN: 0449-2951
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: In continuation of earlier work, another chelating agent containing two different chelating systems in the same molecule (p-nitrobenzeneazotyrosine) was synthesized. 2:1 coordination monomers of this molecule were made and characterized with divalent copper, lead, cobalt, nickel, uranyl, and zinc ions. From these compounds, two types of coordination polymers were synthesized and studied: those involving the ions of one metal bound with both chelating groups in the ligand, and those having two different metals bound in the same polymer chain. Thermal analysis data showed that (among the group under consideration) the bimetallic polymeric chelates have no exceptional stability.
    Additional Material: 7 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1963.100011115
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