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  • 1
    Electronic Resource
    Electronic Resource
    Human urotensin-II is a potent vasoconstrictor and agonist for the orphan receptor GPR14 (1999)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 401 (1999), S. 282-286 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Urotensin-II (U-II) is a vasoactive ‘somatostatin-like’ cyclic peptide which was originally isolated from fish spinal cords, and which has recently been cloned from man. Here we describe the identification of an orphan human G-protein-coupled receptor homologous to rat GPR14 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/45809
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Human AT1 receptor is a single copy gene: Characterization in a stable cell line (1994)
    Springer
    Molecular and cellular biochemistry 131 (1994), S. 75-86 
    Details
    ISSN: 1573-4919
    Keywords: angiotensin II receptor ; gene ; stable cell line ; human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract To address conflicting reports concerning the number of angiotensin II (AII) receptor type 1 (AT1) coding loci in vertebrates, Southern blot analysis was used to determine the genomic representation of AT1 receptor genes in animals comprising a divergent evolutionary spectrum. The data demonstrate that the AT1 receptor gene is present as a single genomic copy in a broad spectrum of animals including human, monkey, dog, cow, rabbit, and chicken. In contrast, members of the rodent taxonomic order contain two genes in their genomes. These two genes may have arisen in rodents as a consequence of a gene duplication event that occurred during evolution following the branching of rodents from the mammalian phylogenetic tree. In order to investigate the properties of the human AT1 receptor in a pure cell system, the recombinant human AT1 receptor was stably expressed in mouse L cells. An isolated cell line, designated LhAT1-D6, was found to express abundant levels of recombinant receptor [430±15 fmol/mg] exhibiting high affinity [KD=0.15±0.02 nM] for [125I][SAR1, IIe8] angiotensin II (SIA). The pharmacological profile of ligands competing for [125I] SIA binding to the expressed recèptor was in accordance with that of the natural receptor. Radioligand binding of the expressed receptor was decreased in the presence of the non-hydrolyzable analog of GTP, guanosine 5′-(γ-thio) triphosphate [GTPγS]. Angiotensin II evoked a rapid efflux of45Ca2+ from LhAT1-D6 cells that was blocked by AT1 receptor specific antagonists. In addition, AII inhibited forskolin-stimulated cAMP accumulation in these cells which was blocked by the AT-1 antagonist. Thus, the LhAT1-D6 cell line provides a powerful tool to explore the human AT1 receptor regulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01075727
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning of a functional human adenine phosphoribosyltransferase (APRT) gene: Identification of a restriction fragment length polymorphism and preliminary analysis of DNAs from APRT-deficient families and cell mutants (1984)
    Springer
    Somatic cell and molecular genetics 10 (1984), S. 359-367 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A complete human APRT gene has been isolated from a lambda phage genomic library using cloned mouse APRT DNA as a probe. The human gene, contained in a recombinant lambda phage designated λHuap15, is functional by virtue of its capacity to transfer human APRT activity to Aprt− mouse recipient cells after phage-mediated transfection. Digestion of λHuap15 DNA with BamH1 generated a 2.2-kb fragment that is the only fragment of eight produced to hybridize with the mouse APRT gene. This 2.2-kb BamH1 fragment is a unique, single copy sequence, and has been used to identify a restriction fragment length polymorphism (RFLP) associated with the APRT locus. Taq1 digestion and Southern blot analysis of DNAs from 49 unrelated individuals produced three different patterns. DNAs of 30 individuals produced a restriction pattern of three labeled fragments about 500 bp, 600 bp, and 2.1 kb in size, which is characteristic for individuals homozygous for the more common allele. Two individuals homozygous for the less frequent allele displayed labeled fragments of 500 bp and 2.7 kb. The remaining 17 DNA samples produced all four labeled bands as expected for heterozygous individuals. The frequency of heterozygotes in the population is about 35%, while the frequency of the less common allele is about 0.21. Restriction enzyme analysis of DNAs from two APRT-deficient brothers and from an unrelated heterozygote revealed no gross deletions or rearrangements, nor the Taq1 polymorphism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01535631
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    Paper (German National Licenses)
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