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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Intensive care medicine 20 (1994), S. S1 
    ISSN: 1432-1238
    Keywords: Intensive care unit ; Infection ; Antibiotic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Patients in intensive care units (ICUs) are a small subgroup of all hospitalized patients, but they account for approximately 25% of all hospital infections. Nosocomial infection rates among ICU patients are 5–10 times higher than among general ward patients. ICU infection rates are higher due to complex interactions between the patients' underlying disease, severity of illness, type of ICU, duration of stay, and invasive devices used. Antimicrobial resistance is a major clinical problem despite potent and newer antibiotics. Organisms that pose a clinically significant resistance problem among ICU patients include methicillin-resistant staphylococci, enterococci, a wide variety of enterobacteriaceae,Pseudomonas aeruginosa, Pseudomonas cepacia, Xanthomonas maltophila, Acinetobacter andCandida species. Traditional infection control measures include identification of reservoirs, halting transmission between patients, stopping progression from colonization to infection and modifying host risk. In addition, sound selection procedures and guidelines for antibiotic usage are necessary to control the spread of multi-resistant micro-organisms.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Digestive diseases and sciences 27 (1982), S. 955-956 
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The aim of this study was to clinically validate a heminested polymerase chain reaction (PCR) method, based on the IS6110 insertion segment of Mycobacterium tuberculosis complex, for the diagnosis of tuberculosis. Samples of pulmonary, extrapulmonary and blood origin were collected prospectively from 331 patients. All samples were processed to detect acid-fast bacilli by direct stain, culture and PCR. The gold standard comparison was a clinically based final case definition of tuberculosis corresponding to group 3 of the American Thoracic Society's classification system. The sensitivities of stain, culture and PCR were 41%, 65% and 59%, respectively. Overall specificity exceeded 97% for all techniques. The combination of PCR and direct stain achieved a sensitivity similar to that of culture alone. The PCR method detected 74 of 95 (78%) culture-positive results. In a hospital setting, PCR could be a useful, reliable tool for diagnosis of tuberculosis and may be introduced as a complementary routine diagnostic laboratory method.
    Type of Medium: Electronic Resource
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