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Involvement of intestinal inducible nitric oxide synthase (iNOS) in the early stages of murine salmonellosis (2003)

Giacomodonato, Mónica N. ; Goren, Nora B. ; Sordelli, Daniel O. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 223 (2003), S. 0

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ISSN: 1574-6968

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Local induction of inducible nitric oxide synthase (iNOS) and apoptosis was examined in the intestine of mice infected with virulent Salmonella enterica serovar Enteritidis 5694 (S. enteritidis) and its attenuated derivative mutant E/1/3. Both, intestinal iNOS mRNA expression and iNOS activity showed a peak at 4 h only in animals receiving the virulent S. enteritidis. Aminoguanidine treatment abrogated intestinal epithelial damage produced by virulent S. enteritidis and diminished apoptosis at the tips of the villi. Unlike the virulent strain, mutant E/1/3 induced massive iNOS expression in Peyer's patches, these findings may be related to its protective capacity. Our results suggest that intestinal iNOS participates in the early response to intestinal infection and that the final effect depends on the nature of the insult.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1016/S0378-1097(03)00385-9

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Lipopolysaccharide Directly Affects Pancreatic Acinar Cells (2000)

Vaccaro, María I. ; Calvo, Ezequiel L. ; Suburo, Angela M. ; [et al.]

Springer

Digestive diseases and sciences 45 (2000), S. 915-926

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ISSN: 1573-2568

Keywords: pancreatic acinar cells ; acute pancreatitis ; lipopolysaccharide ; pancreatitis-associated protein ; cytokines

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract We have explored whether lypopolisaccharide (LPS, endotoxin) induces pancreatic injury on pancreatic acinar cells both in vivo and in vitro. Wistar male rats were treated with four intraperitoneal injections of 10 mg/kg LPS, and AR4-2J cells were exposed to increasing doses of LPS. Expression of pancreatitis-associated-protein (PAP) mRNA was strongly induced in AR4-2J cells exposed to LPS, while amylase mRNA was reduced. LPS also induced apoptosis and expression of TNF-α, IL-1β, and IL-8 mRNA in AR4-2J cells. The in vivo effect of LPS showed structural signs of cellular damage, including numerous cytoplasmic vacuoles, severe nuclear alterations, and high expression of PAP mRNA. This study demonstrated that LPS induced pancreatic damage by directly affecting the pancreatic acinar cells. The role of LPS in the pathophysiology of acute pancreatitis may be partly due to the effect LPS has on the acinar cell.