ISSN:
1573-2568
Keywords:
pancreatic acinar cells
;
acute pancreatitis
;
lipopolysaccharide
;
pancreatitis-associated protein
;
cytokines
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract We have explored whether lypopolisaccharide (LPS, endotoxin) induces pancreatic injury on pancreatic acinar cells both in vivo and in vitro. Wistar male rats were treated with four intraperitoneal injections of 10 mg/kg LPS, and AR4-2J cells were exposed to increasing doses of LPS. Expression of pancreatitis-associated-protein (PAP) mRNA was strongly induced in AR4-2J cells exposed to LPS, while amylase mRNA was reduced. LPS also induced apoptosis and expression of TNF-α, IL-1β, and IL-8 mRNA in AR4-2J cells. The in vivo effect of LPS showed structural signs of cellular damage, including numerous cytoplasmic vacuoles, severe nuclear alterations, and high expression of PAP mRNA. This study demonstrated that LPS induced pancreatic damage by directly affecting the pancreatic acinar cells. The role of LPS in the pathophysiology of acute pancreatitis may be partly due to the effect LPS has on the acinar cell.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005521007609
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