Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We studied collagen expressed by skin fibroblasts in culture, and performed immunocytochemical, ultrastructural and stereological analysis of dermis from a child with signs reminiscent of a mild Ehlers–Danlos syndrome (EDS) type IV and severe periodontitis. No alterations in types I and II collagen synthesis and secretion, or serum levels of type III procollagen aminoterminal propeptide were found, and morphological studies revealed non-specific alterations of collagen and elastic components observed in many connective tissue disorders.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Ultrastructure Research and Molecular Structure Research 102 (1989), S. 132-138 
    ISSN: 0889-1605
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Marine biology 22 (1973), S. 225-230 
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Electron-microscope observations on ultrathin sections of Mytilus galloprovincialis byssus have revealed the existence of fungal hyphae. The ultrastructural features of these hyphae identify them as ascomycetes. A saprophytic nature is suggested for this fungus, and its probable role in the degradation of the byssus is discussed.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 81 (1984), S. 561-565 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pedal ganglion is a peripheral ganglion which gives rise to the innervation for both the somatic and visceral organs of the Mytilus foot. In the present study, different histofluorescence methods for the demonstration of monamines (formaldehyde-glutaraldehyde followed by polyethylene glycol embedding; aluminium-formaldehyde; Falck) and acetylcholinesterase histochemistry were applied in order to characterize the neuronal population of the ganglion. The fluorescence methods employed showed that the cortical region of the pedal ganglion is composed of roundish cells; these mainly contained an orange autofluorescent pigment. Yellow-fluorescing cells were scattered in the anterior region of the cortex, but they were more numerous and arranged in clusters in the posterior region. Greenfluorescing cells were mainly located at the border between the cortex and neuropile and in the neuropile itself, where a rich plexus of beaded green-fluorescing fibres was also present. Of the three methods, that using formaldehydeglutaraldehyde followed by embedding in polyethylene glycol gave the best preservation of morphological details. Acetylcholinesterase histochemistry showed the presence of positive cells and fibres mainly in the anterior region of the ganglion.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The surface morphology of the byssus of Mytilus galloprovincialis was investigated with the scanning electron microscope (SEM), and its structure studied by microdissection and on histological sections obtained with the freezing microtome. A detailed description of the structure of the byssus is given. The stem should be regarded as an organ that undergoes extensive age-related structural changes, chiefly consisting of variations in the number and arrangement of its threads. The stem and the thread differ considerably in structure, as expected from their different mechanical function. The stem consists of an outer layer of laminar “cuffs” wrapped around a cylindrical “core”. The threads are made up of different portions, i.e., (1) a rigid portion (cuff), by which they are attached to the stem; (2) a straight, elastic proximal portion where a corrugated sheath envelops a central rectilinear core; (3) a rigid, cylindrical distal portion; (4) an adhesive plate which anchors to the substratum. On the strength of the above, the function of the byssus and its formation process are discussed.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 166 (1976), S. 219-234 
    ISSN: 1432-0878
    Keywords: Electron microscopy ; Byssus ; Collagen ; Mytilus galloprovincialis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the byssus of Mytilus galloprovincialis was analysed by transmission electron microscopy in thin sections of either embedded or frozen samples. All parts of the byssus (stem core laminae, stem outer laminae, threads proximal and distal parts) appear to be formed by the same basic filamentous components organized in different ways at the submicroscopic level and embedded in a variable quantity of matrix. The filaments appear to consist of a central electron-lucent zone (3 nm in diameter), surrounded by an electron-dense rim (total diameter 7 nm). The matrix has a granular or microfilamentous structure. The stem and the threads differ greatly in their submicroscopic organization, but their basic constituents (filaments and matrix) are similar. Peculiar filamentous banded elements (FBE) were found mainly in the stem outer laminae. A relation between the ultrastructure and mechanical properties of the different parts of the byssus was established. The presence of collagen is discussed; since no morphological evidence of any of the known forms of collagen organization was revealed by electron microscopy, it is suggested that byssus collagen may be localized in the matrix and in the FBE.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 249 (1987), S. 111-116 
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Serotonin ; Mytilus galloprovincialis ; pedal ganglion ; Invertebrate nervous system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Serotonergic cell bodies and fibers were identified in the pedal ganglia of Mytilus gattoprovincialis with a serum raised against serotonin and the unlabelled peroxidase-antiperoxidase pre- and post-embedding methods. Examination of Vibratome and serial semithin sections showed that most reactive perikarya are located in the ganglionic cortex, being mainly concentrated at the medial aspect of the postero-dorsal portion of the ganglia. Immunoreactive fibers form a dense network in the neuropil, extend throughout the commissure and run parallel in the nerves and connective tracts. The morphology of serotonin-positive cells compared with that of Golgi-impregnated neurons allows the identification of a main population of unipolar, probably projecting neurons and of smaller multipolar cells likely representing local circuit elements. The ultrastructure of labelled neurons is comparable to that of serotonergic cells described in both vertebrate and invertebrate nervous systems.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    ISSN: 1432-0878
    Keywords: Dermis ; Collagen fibers ; Extracellular matrix ; Fibronectin ; Immunocytochemistry ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of collagen types I, III, IV, and of fibronectin has been studied in the human dermis by light and electron-microscopic immunocytochemistry, using affinity purified primary antibodies and tetramethylrhodamine isothiocyanate-conjugated secondary antibodies. Type I collagen was present in all collagen fibers of both papillary and reticular dermis, but collagen fibrils, which could be resolved as discrete entities, were labeled with different intensity. Type III collagen codistributed with type I in the collagen fibers, besides being concentrated around blood vessels and skin appendages. Coexistence of type I and type III collagens in the collagen fibrils of the whole dermis was confirmed by ultrastructural double-labelling experiments using colloidal immunogold as a probe. Type IV collagen was detected in all basement membranes. Fibronectin was distributed in patches among collagen fibers and was associated with all basement membranes, while a weaker positive reaction was observed in collagen fibers. Ageing caused the thinning of collagen fibers, chiefly in the recticular dermis. The labeling pattern of both type I and III collagens did not change in skin samples from patients of up to 79 years of age, but immunoreactivity for type III collagen increased in comparison to younger skins. A loss of fibronectin, likely related to the decreased morphogenetic activity of tissues, was observed with age.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 9
    ISSN: 1432-0878
    Keywords: Serotonin (5-HT) ; Substance P ; GABA ; Immunocytochemistry ; Invertebrate peripheral nervous system ; Mytilus galloprovincialis (Mollusca)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunocytochemical methods were applied to study the distribution of putative neurotransmitters (5-HT, substance P, GABA, glutamate and aspartate) in the nerve plexuses of the foot and the anterior byssus retractor muscle (ABRM) of Mytilus galloprovincialis (Mollusca, Bivalvia). The foot presents extensive nerve plexuses containing 5-HT and substance P-like immunoreactive material with a similar distribution beneath the surface epithelium, around the vessels and in the glandular regions. Coexistence of the two putative neurotransmitters was observed in a few nerve fibers, Conversely, muscle fibers, both in the foot and in the ABRM, are innervated only by 5-HT-positive fibers, while substance P-like material is present only in the networks of the ABRM epimysial sheath. Immunoreactivity for glutamate and aspartate was not demonstrated, while rare GABA-positive nerve cells and fibers were found only in the foot. The results of this investigation provide a morphological background to previous physiological studies on 5-HT in the nervous system of bivalve molluscs. Moreover, they confirm that the nervous system of Mytilus contains a remarkable amount of a substance related to the vertebrate tachykinin family.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Neurological sciences 19 (1998), S. 249-253 
    ISSN: 1590-3478
    Keywords: Pericellular nets ; Golgi ; Ramón y Cajal ; History of neuroscience ; Extracellular matrix
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Sommario Gli involucri perineuronali sono strutture a rate che avvolgono i pirenofori e i grossi dendriti di numerose popolazioni neuronali. Scoperti da Camillo Golgi, che li descrisse dettagliatamente net 1898, furono studiati intensamente dai maggiori neuroistologi dell’epoca per circa un ventennio. L’opinione di Ramón y Cajal the gli involucri perineuronali fossero artefatti delta fissazione interruppe la prima fase di ricerche. Solo pochi ricercatori, fra cui i neurologi Besta e Belloni, continuarono gli studi fino agli anni Trenta, documentando la morfologia degli involucri perineuronali nell’ encefalo di diversi mammiferi e dell’ uomo in condizioni normali e patologiche. Solo dopo un cinquantennio, i progressi in campo citochimico consentirono di chiarire non solo l’effettiva esistenza degli involucri perineuronali, ma anche la loro composizione chimica, dimostrando the essi sono complesse organizzazioni di molecole delta matrice extracellulare, principalmente glicoproteine e proteoglicani. Le ricerche sugli involucri perineuronali coinvolgono oggi numerosi gruppi di ricerca impegnati a chiarirne le proprietà biologiche e it significato funzionale.
    Notes: Abstract Perineuronal nets are reticular structures enwrapping cell bodies and the largest dendrites of several neuronal populations. Discovered by Camillo Golgi, who described them in detail in 1898, they were intensely studied by the most famous contemporary neurohistologists for about twenty years. The opinion of Ramón y Cajal that perineuronal nets were a fixation artifact ended the first period of studies. Only a few researchers, among whom the Italian neurologists Besta and Belloni, went on with their studies up to the 1930s documenting the morphology of perineuronal nets of different mammals and of man both in normal and in pathological conditions. Only after about fifty years, the advances in the field of cytochemistry allowed the elucidation of not only the actual existence of perineuronal nets, but also their chemical nature, showing conclusively that they are complex organisations of extracellular matrix molecules, namely glycoproteins and proteoglycans. The research on perineuronal nets today involves several groups engaged to elucidate their biological properties and functional role.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...