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  • 1
    Electronic Resource
    Electronic Resource
    On the variability of the 3′ terminal sequence of the turnip mosaic virus genome (1992)
    Springer
    Archives of virology 126 (1992), S. 231-238 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The sequence of the 3′-terminal 1223 nucleotides (nts) of a Japanese isolate of turnip mosaic virus (TuMV-Jap) RNA has been determined. The sequence reveals a single open reading frame (ORF) which terminates at a position 212 nts upstream of the 3′ poly(A)-tract. Determination of the N-terminal amino acids of TuMV-Jap coat protein (CP) mapped the CP cistron within this ORF and revealed a Glu-Ala dipeptide sequence as the putative cleavage site by which the CP is released from the viral polyprotein. The predicted amino acid sequence of the TuMV-Jap CP shows 97.2% identity with that of a Canadian isolate of TuMV (TuMV-Can) and 99% with a second, Chinese, isolate (TuMV-Chi). However, the 3′-terminal non-translated region (NTR) of TuMV-Jap RNA is significantly shorter (212 nts) than the 3′-NTR of TuMV-Can RNA (668 nts), but of equal length as the 3′-NTR of the TuMV-Chi isolate which also measures 212 nts. The 3′-NTRs of both the TuMV-Jap and TuMV-Chi RNAs show homology with the first 201 nucleotides of the TuMV-Can RNA 3′-NTR. A search in the EMBL nucleotide sequence database revealed that the 467 nt-long unique extension of the 3′-NTR of TuMV-Can RNA has 89.8% homology to a part of the chloroplast ribosomal protein 12 gene (rsp 12-gene). Irrespective of the origin of this extra sequence in the reported TuMV-Can sequence, which may have been introduced by a genuine RNA recombination event, it is concluded that the standard TuMV genome has a CP gene of 864 nts and an conserved 3′-NTR of approximately 212 nucleotides in length.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01309697
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  • 2
    Electronic Resource
    Electronic Resource
    Nucleotide sequence of the 3′ terminal region of the genome of four Lettuce mosaic virus isolates from Greece and Yemen (1999)
    Springer
    Archives of virology 144 (1999), S. 1619-1626 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  Lettuce mosaic virus (LMV) is an economically important Potyvirus causing a severe disease of commercial lettuce crops. Based on molecular data, three phylogenetic groups of isolates have previously been discriminated, reflecting their geographical origin (Western Europe-California, Greece, or Yemen). Sequence information for the entire coat protein domain was only available for one of the Western Europe-California phylogenetic group. We have now sequenced the 3′ terminal region of the genome LMV-Gr4, -Gr5 and -GrB, isolates which belong to the Greek phylogenetic group and of LMV-Yar, the sole known representative of the third LMV phylogenetic group. The region sequenced encodes the last 62 amino-acids of the polymerase and the entire coat protein of the four isolates, plus the 3′ non-translated region of LMV-Gr5 and -Yar. The Greek and Yemenite isolates studied are all very aggressive on lettuce, are able to overcome the resistance genes mo1 1 and mo1 2 and belong to the two phylogenetic groups which have so far been only partially characterised. As for other Potyviruses, the core and the C-terminal regions of the coat protein are highly conserved among all isolates whereas the N-terminus is more variable. No amino acid change in the coat protein or carboxy-terminal part of the polymerase could be related to the resistance-breaking properties of the isolates analysed. The sequences obtained provide the basis for the rapid typing of LMV isolates using the restriction pattern of segments of cDNA amplified by PCR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050615
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  • 3
    Electronic Resource
    Electronic Resource
    Taxonomic relationships between distinct potato virus Y isolates based on detailed comparisons of the viral coat proteins and 3′-nontranslated regions (1993)
    Springer
    Archives of virology 131 (1993), S. 361-375 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Detailed comparisons were made of the sequences of the coat protein (CP) cistrons and 3′-nontranslated regions (3′-NTR) of 21 (geographically) distinct isolates of potato virus Y (PVY) and a virus isolate initially described as pepper mottle virus (PepMoV). Multiple sequence alignments and phylogenetic relationships based on these alignments resulted into a subgrouping of virus isolates which largely corresponded with the historical strain differentiation based on biological criteria as host range, symptomatology and serology. Virus isolates belonging to the same subgroup shared a number of characteristic CP amino acid and 3′-NTR nucleotide residues indicating that, by using sequences from the 3′-terminal region of the potyvirus genome, a distinction could be made between different isolates of one virus species as well as between different virus species. RNA secondary structure analysis of the 3′-NTR of twelve PVY isolates revealed four major stem-loop structures of which, surprisingly, the loop sequences gave a similar clustering of isolates as resulting from the overall comparisons of CP and 3′-NTR sequences. This implies a biological significance of these structural elements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01378638
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  • 4
    Electronic Resource
    Electronic Resource
    Resistance to potato virus Y (pathotype 1–2) in Capsicum annuum and Capsicum chinense is controlled by two independent major genes (1996)
    Springer
    Euphytica 87 (1996), S. 53-58 
    Details
    ISSN: 1573-5060
    Keywords: Capsicum spp. ; pepper ; potato virus Y ; potyvirus ; pathotype ; host resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The genetic basis of the resistance to a pepper strain of potato virus Y (PVY pathotype 1–2) was investigated in two lines: Capsicum annuum ‘Criollo de Morellos 334’ and Capsicum chinense ‘PI 159236’. F1, backcrosses, and F2 populations were produced using C. annuum ‘Magda’ as the susceptible parent. Segregation ratios indicated that the resistance in C. annuum ‘Criollo de Morellos 334’ is governed by a nuclear, single dominant gene with a very high but incomplete penetrance. On the other hand, the resistant response of C. chinense ‘PI 159236’ is associated with the presence of a major recessive gene with an apparently complete penetrance. The failure in recovering or detecting PVY from/in the inoculated leaves of both resistant genotypes is strongly suggestive that both genes are acting by preventing the multiplication of the virus in the leaf tissue. Both mechanisms of resistance closely resemble either an operational immunity or a single-cell hypersensitive reaction to PVY pathotype 1–2. The name Ry 1–2 is suggested for the resistance gene from ‘Criollo de Morellos 334’. The gene for resistance to PVY pathotype 1–2 identified in C. chinense ‘PI 159236’ may be the same designated et c1, which is mentioned to be allelic to the et av locus. The phenotypic expression of the et av gene was characterized in C. annuum ‘Avelar’ as a tolerant but not immune or hypersensitive reaction against some potyviruses in Florida. These data suggest that a critical reexamination of the allelic relationships at the locus et should be performed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022964
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  • 5
    Electronic Resource
    Electronic Resource
    Characteristics of a resistance-breaking isolate of potato virus Y causing potato tuber necrotic ringspot disease (1994)
    Springer
    European journal of plant pathology 100 (1994), S. 347-356 
    Details
    ISSN: 1573-8469
    Keywords: Potyviridae ; potato virus Y ; detection ; monoclonal antibodies ; ELISA ; virus transmission ; aphids ; transgenic resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An Austrian isolate of potato virus YNTN, the causal agent of potato tuber necrotic ringspot disease (PTNRD), was serologically compared with seven Dutch PVYN isolates. Using polyclonal and monoclonal antibodies, it was found indistinguishable from PVYN. Determination of the nucleotide sequence of the coat protein cistron and comparison of the deduced amino acid sequence with coat protein sequences of other potyviruses revealed a high level of homology with PVYN coat protein sequences. This confirmed the close taxonomic relationship of PVYNTN with the PVYN subgroup of potato virus Y. PVYNTN is able to overcome all resistance genes known so far in commercial potato cultivars. Remarkably, transgenic PVY-protected tobacco plants are also resistant to PVYNTN infection upon mechanical and aphid-mediated inoculation. These experiments indicate that genetically engineered resistance offers great potential in protection of potato to new aggressive strains of PVYN.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01876445
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