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  • 1
    Electronic Resource
    Electronic Resource
    In situ localization of the transcripts of a homeobox gene in the honeybee Apis mellifera L. (Hymenoptera) (1988)
    Springer
    Development genes and evolution 197 (1988), S. 269-274 
    Details
    ISSN: 1432-041X
    Keywords: Apis mellifera ; Homeobox genes ; Dfd ; In situ hybridization ; Blastoderm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated and characterized a homeoboxcontaining gene from the honeybee Apis mellifera. Its homeobox region shows a high degree of sequence similarity to the homeobox of the Drosophila gene Deformed (Dfd). At the DNA level 82% of the basepairs are the same, whereas the putative amino acid sequences are identical between the bee and the fruitfly genes. Similarity is also present 5′ and 3′ to the homeobox. Using this isolate as a probe we have performed in situ hybridization on sections from blastoderm-stage embryos of the honeybee Apis mellifera. In early blastoderm stages we found a rather irregular pattern of labelled nuclei. In middle stages we found silver grains over each nucleus and also over the cytoplasm in a belt of blastoderm cells in the prospective gnathal region. These results indicate that the Deformed genes from honeybee and fruitfly are homologous both with respect to their DNA sequence and their spatial and temporal pattern of expression during embryogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380020
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Two major RNA products are transcribed from heat-shock locus 93D ofDrosophila melanogaster (1985)
    Springer
    Chromosoma 93 (1985), S. 17-20 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The heat-shock locus 93D fromDrosophila melanogaster has recently become available for a molecular analysis. We established a restriction site map of a recombinant DNA clone covering the major part of heat-shock locus 93D. This clone includes part of a repetitive Taq I region and neighbouring unique sequences. The portion of the Taq I repeat analysed consists of tandemly arranged sequence blocks of about 280 base pairs (bp) in length. Using genomic and cDNA as hybridization probes we examined the transcription of 93D in 2- to 4-day-old flies. We identified two major RNA classes enhanced after heat shock, namely nonpolyadenylated transcripts of heterogeneous length derived from the repetitive region and one discrete polyadenylated transcript in spliced and unspliced form from the neighbouring unique region. The occurrence of a highly heterogeneous poly(A)− transcript and high levels of an unspliced discrete poly(A)+ species suggests unusual mechanisms of transcription regulation in the 93D region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01259440
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The unusual structure of heat shock locus 2-48B in Drosophila hydei (1984)
    Springer
    Molecular genetics and genomics 197 (1984), S. 392-398 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have previously isolated a 500 bp-long cDNA clone, NO9-15, which is derived from a nuclear transcript originating from the heat shock locus 2-48B of Drosophila hydei (Peters et al. 1982). Sequence analysis shows that this clone carries 4 complete copies and 1 partial copy of a 115 bp repeat unit. The repeats are closely homologous with a maximal sequence divergence of about 10%. The sequence does not contain an open reading frame. The genomic organization of heat shock locus 2-48B, as probed with the cloned cDNA sequence NO9-15, is highly polymorphic. Four different allelic arrangements have been found in different inbred strains. A number of genomic clones isolated from region 2-48B, both in phage lambda and in cosmid vectors, all differ in length, mainly due to varying numbers of the NO9-15 repeat unit. These differences are found primarily in the proximal region of the locus. The transcribed region of these clones includes the distal sequence flanking the NO9-15 repeat as well as the NO9-15 repeat itself. An oligo A stretch was found between the distal flanking sequence and the NO9-15 repeat region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329934
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Heat-shock locus 93D of Drosophila melanogaster: An RNA with limited coding capacity accumulates precursor transcripts after heat shock (1986)
    Springer
    Molecular genetics and genomics 204 (1986), S. 334-340 
    Details
    ISSN: 1617-4623
    Keywords: D. melanogaster ; Heat shock ; RNA splicing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Heat-shock locus 93D of Drosophila melanogaster consists of an internally repetitive and a neighbouring unique region. The unique part contains a promoter that is already active in non-heat-shocked cells but shows fivefold enhanced transcription after stress induction. In third instar larvae, a series of 93D-derived RNA products appear, which might be the result of incomplete processing events. The major RNA species (1.2 kb) is the splicing product of a poly(A)+-containing primary transcript of 1.9 kb. Furthermore, a transcript of high molecular weight is observed, which in addition contains the 3′-flanking 93D-specific ‘TaqI repeat’ sequences. This readthrough transcript is found in the poly(A)+ as well as in the poly(A)- RNA fraction. After severe heat shock, the already limited processing efficiency of 93D RNA is further inhibited. Production of the readthrough transcript, on the other hand, is reduced. DNA sequence analyses of genomic and cDNA sequences reveal that this 93D heat-shock gene contains only a very limited protein-coding capacity. A coding function for the mature 93D heat-shock RNA is therefore questionable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425519
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    Paper (German National Licenses)
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