ISSN:
1436-2813
Keywords:
Key Words Partial hepatectomy
;
Signal transduction
;
Phospholipase C
;
Phospholipase A2
;
Cyclooxygenase
;
Phospholipase D
;
Hepatocyte growth factor
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Prostaglandins (PGs), metabolites of arachidonic acid, and other lipid mediators produced by phospholipases C (PLC) and D (PLD) are thought to play important roles in hepatocyte proliferation. The present study examined lipid-mediated signaling in the rat liver after partial hepatectomy (PH). Rats were killed 1–48 h after 70% PH and the remaining liver tissue was removed. The mRNA and protein levels of some signaling molecules were measured by reverse transcriptase–polymerase chain reaction (RT-PCR) and Western blotting, respectively. The levels of hepatocyte growth factor (HGF) mRNA showed a biphasic change, peaking 3 h and 9 h after PH. The expression of PLCδ4 peaked at 12 h, but no significant changes in the expression of PLCβ1 and PLCγ1 were seen after PH. The enzymes involved in PG production, namely, the expression of cytosolic PLA2 and cyclooxygenase 1 (COX1), remained constant after PH. However, the mRNA of COX2 increased transiently at 3 h, and Western blot analysis showed an increase in COX2 protein at 12 h. The expression of PLD1b peaked at 9 h and PLD1a at 12 h, whereas the expression of PLD2 remained consistent for 24 h. These results suggest that transcriptional controls may act for PLCδ4, PLD1a/b, and COX2 during hepatocyte regeneration after PH.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s005950070102
Permalink