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  • 1
    Electronic Resource
    Electronic Resource
    Respiratory syncytial virus infection: its role in aeroallergen sensitization during the first two years of life (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Pediatric allergy and immunology 7 (1996), S. 0 
    Details
    ISSN: 1399-3038
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Our aim was to study the influence of infection with the respiratory syncytial virus (RSV) in non-hospitalized infants on sensitization to aeroallergens and the early manifestation of atopy. Six hundert and nine infants from the prospective German Multicenter Cohort Study on Atopy were included, 38% of whom had an elevated atopic risk. RSV IgG and IgM antibodies were tested by ELISA with gradient purified RSV antigen. Specific IgE against mites, cat dandruff, birch and grass pollens and relevant nutritional antigens were tested with CAP-RAST-FEIA (Pharmacia, Sweden). Of the cord sera 99% were positive for RSV-IgG, 44. 7% at one year and 64. 2% (n=265) at two years of age. The positivity rate after 12 months varied with the season of birth, the number of siblings and the degree of exposure to tobacco smoke; and correlated closely with attacks of wheezing during infancy. Twenty (2. 8%) children were found to be sensitized against at least one aeroallergen at one year, and 28 (10. 5%) at two years. By the first birthday, mite sensitization (n=3) could only be seen in the RSV-infected children; grass pollen sensitization (n=9) was associated with RSV seropositivity (logistic regression model including the confounders mentioned above: with RSV IgG p=0.04 〉 and IgM p=0.0006), as was birch sensitization (n=5) with RSV IgM (p=0.009). No such differences could be detected at two years. No correlation of RSV seropositivity to any allergic manifestation could be found. We conclude, that it is only in the first year of life, that RSV infection plays a significant role in promoting sensitization against aeroallergens, which do not at this time produce allergic symptoms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3038.1996.tb00107.x
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  • 2
    Electronic Resource
    Electronic Resource
    Studies on the effect of distamycin A on the DNA dependent RNA polymerase system
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 43 (1971), S. 617-624 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0006-291X(71)90659-0
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  • 3
    Electronic Resource
    Electronic Resource
    Epidemiology of rotavirus gastroenteritis in infants from the area of Bochum as revealed by electrophoresis of genome RNA (1984)
    Springer
    European journal of pediatrics 143 (1984), S. 128-132 
    Details
    ISSN: 1432-1076
    Keywords: Gastroenteritis ; Rotaviruses ; ELISA ; RNA electropherotypes ; Epidemiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Rotaviruses were detected by enzyme-linked immunosorbent assay (ELISA) in stool samples from 504 infants admitted with gastroenteritis to the children's hospital of the University Bochum in the period from 1979 to 1984. In most infants rotaviruses persisted in the stools for 6–8 days. Rotavirus RNA was extracted from stool samples from 203 infants. By acrylamide gel electrophoresis, bands characteristic of rotavirus double-stranded RNA were detected in 172 (85%) of these 203 samples in which the presence of rotavirus had been demonstrated by ELISA. Of the RNA-positive samples 87% had a “long” RNA pattern. Six different electropherotypes co-circulated and one electropherotype predominated for more than 4 years. In stool samples of one infant 12 genome segments were found, suggesting the possibility of simultaneous infection by more than one electropherotype or the occurrence of modification in the length of RNA segments during infection. In another infant the sequential infection by two different electropherotypes was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00445800
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  • 4
    Electronic Resource
    Electronic Resource
    Die Wirkung einer Kombination von N1-β-Phenyläthylbiguanid (DBI, W32) mit Insulin und Tolbutamid (D 860) auf den Stoffwechsel des Meerschweinchens (1961)
    Springer
    Journal of molecular medicine 39 (1961), S. 1080-1083 
    Details
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01487654
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  • 5
    Electronic Resource
    Electronic Resource
    Der Pyridinnucleotid-Stoffwechsel in der Rattenleber während der Carcinogenese (1966)
    Springer
    Journal of cancer research and clinical oncology 68 (1966), S. 156-163 
    Details
    ISSN: 1432-1335
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The carcinogenic agent, N-nitroso-morpholine, was fed to rats for 200 days. During this time the metabolism of pyridine nucleotides of the liver was studied. The content of NAD and NADP remained constant; the reduced pyridine nucleotides decreased continuously from the 100 th day. A few days after beginning the feedings an injection of nicotinic acid amide caused the NAD to reach a value twice that of the controls. This effect was lost after the 140 th day. After injection of the nicotinic acid amide, actinomycin D influenced the synthesis of the pyridine nucleotides significantly in the regenerating liver, only slightly in the precancerous liver, and not in the normal liver.
    Notes: Zusammenfassung 200 Tage wurde die carcinogene Substanz N-Nitroso-morpholin an Ratten verfüttert. Während dieser Zeit untersuchten wir den Pyridinnucleotid-Stoffwechsel der Leber. Der Gehalt an NAD und NADP blieb konstant, während die reduzierten Pyridinnucleotide vom 100. Tag an beständig abnahmen. Wenige Tage nach Beginn der Fütterung bewirkte eine Injektion von Nicotinsäureamid, daß das NAD den doppelten Wert der Kontrolle erreichte. Dieser Effekt verlor sich wieder nach dem 140. Tag. Actinomycin D beeinflußte die durch eine Injektion von Nicotinsäureamid hervorgerufene Synthese der Pyridinnucleotide nicht in der normalen, gering in der präcancerösen und erheblich in der regenerierenden Leber.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00525157
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  • 6
    Electronic Resource
    Electronic Resource
    Untersuchungen über den Nucleinsäurestoffwechsel von Affennierengewebe-Kulturzellen nach Infektion mit SV 40 (1966)
    Springer
    Archives of virology 19 (1966), S. 342-350 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Viral DNA isolated from purified SV 40 virus according to different procedures cannot be differentiated from normal cell DNA by chromatography on MAK-columns. Heat-treated viral DNA elutes at the same NaCl-concentrations as native viral DNA, whereas heat-treated cell DNA is not eluted under the described conditions.
    Notes: Zusammenfassung Aus gereinigtem SV 40-Virus nach verschiedenen Verfahren isolierte native DNS unterschied sich chromatographisch an der MAK-Säule nicht von nativer DNS, die aus nicht infizierten primären Affennierengewebekulturen gewonnen wurde. Nach thermischer Denaturierung und anschließender Renaturierung wird die Virus-DNS wie in nativer Form von der Säule eluiert, nicht dagegen die gleichartig behandelte Zell-DNS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01241855
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  • 7
    Electronic Resource
    Electronic Resource
    Untersuchungen über den Nucleinsäurestoffwechsel von Affennierengewebe-Kulturzellen nach Infektion mit SV 40 (1966)
    Springer
    Archives of virology 19 (1966), S. 351-360 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary After infection of primary cultures of green monkey (Cerc. aeth.) kidney cells with SV 40 the incorporation of14C-thymidine is increased in comparison to uninfected cultures. The nature of DNA synthesized after infection was analysed by chromatography on MAK-columns. About 10% of the total DNA extracted from infected cultures show the chromatographic characteristics of viral DNA, most of the extracted DNA behave like cell DNA in this respect. The problem whether the infection of monkey kidney cell cultures induces the synthesis of cell DNA is discussed. The incorporation of14C-Uridin into RNA during SV 40 development was not significantly different from control cultures.
    Notes: Zusammenfassung Nierengewebekulturen (Cerc. aeth.) zeigen 72 bis 120 Stunden nach Infektion mit SV 40 einen gesteigerten Einbau von14C-Thymidin im Vergleich zu nicht infizierten Kontrollkulturen. Bei chromatographischer Untersuchung der aus infizierten Kulturen extrahierbaren DNS unter Verwendung der MAK-Säule wiesen nur etwa 10% dieser DNS die Charakteristika von Virus-DNS auf, die übrige DNS verhält sich in dieser Hinsicht wie Zell-DNS. Die Frage, wieweit die Infektion mit SV 40 die Synthese von Zell-DNS in infizierten Zellen induziert, wird diskutiert. Die Einbaurate von14C-Uridin in die RNS zeigte zwischen infizierten und nicht infizierten Kulturen keine wesentlichen Unterschiede.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01241856
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  • 8
    Electronic Resource
    Electronic Resource
    Untersuchungen über den Nucleinsäurestoffwechsel von Affennierengewebe-Kulturzellen nach Infektion mit SV-40 (1967)
    Springer
    Archives of virology 21 (1967), S. 265-275 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary SV-40 infected monkey kidney tissue cultures (Cerc, aeth.) were labeled with 2-14C-thymidine from the 72nd to the 74th hour after infection. 74, 98 and 122 hours after infection all DNA was extracted from the cultures and investigated by zone centrifugation. No changes in the molecular size of the DNA extracted at the different times were detectable. X-irradiated monkey kidney tissue cultures synthesized as much SV-40 virus as non-irradiated cultures. In X-irradiated tissue cultures as well as in non-irradiated cultures the synthesis of a DNA of the cellular type was stimulated in addition to the synthesis of viral DNA.
    Notes: Zusammenfassung Merengewebekulturen (Cerc, aeth.) wurden nach Infektion mit SV-40 von der 72. bis 74. Stunde p.i. mit 2-14C-Thymidin markiert. 74, 98 und 122 Stunden p.i. wurde die DNS aus den Kulturen extrahiert und mit Hilfe der Bandenzentrifugation untersucht. Veränderungen der Molekülgroße der zu diesen Zeiten extrahierten DNS waren nicht feststellbar. Röntgenbestrahlte Nierengewebekulturen bildeten nach der Infektion mit SV-40 im gleichen Ausmaß Virus wie nicht bestrahlte Zellkulturen. Auch in röntgenbestrahlten Gewebekulturen wurde neben Virus-DNS eine DNS zellulären Typs gebildet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01241450
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  • 9
    Electronic Resource
    Electronic Resource
    Untersuchungen über den Nucleinsäurestoffwechsel von Affennierengewebekulturzellen nach Infektion mit SV 40 IV. Einfluß von Antimetaboliten und Röntgenstrahlen auf die Bildung von Enzymen des Nucleinsäurestoffwechsels (1967)
    Springer
    Archives of virology 22 (1967), S. 305-315 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The infection of primary cultures of green monkey kidney cells (AGMK) with SV40 induced TdR-kinase- and DNA-polymerase activity. The RNA-polymerase-activity was not altered by SV40. Puromycin and actinomycin treatment inhibited the TdR-kinase induction by SV40. JUDR did not prevent the induction of TdR-kinase and DNA-polymerase by SV40. Increased levels of these enzymes in infected cultures were also seen, if the cultures were X-irradiated before infection.
    Notes: Zusammenfassung In stationären Affennierengewebekulturen (Cercopithecus aethiops) werden nach SV40-Infektion die Enzymaktivitäten der TdR-Kinase und DNS-Polymerase gesteigert. Die RNS-Polymerase-Aktivität wird durch den SV40-Infekt nicht beeinflußt. Puromycin und Actinomycin hemmen die Stimulierung der TdR-Kinase deutlich. JUDR vermag die Steigerung der Enzymaktivitäten der TdR-Kinase und DNS-Polymerase in infizierten Kulturen nicht zu verhindern. Erhöhte Aktivitäten der beiden Enzyme sind ebenfalls in infizierten Kulturen nachweisbar, wenn die Kulturen vor der Infektion röntgenbestrahlt worden waren.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01242951
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  • 10
    Electronic Resource
    Electronic Resource
    Monoclonal antipeptide antibodies recognize epitopes upon VP4 and VP7 of simian rotavirus SA11 in infected MA104 cells (1992)
    Springer
    Archives of virology 122 (1992), S. 281-291 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To study morphogenetic events of rotavirus SA11-infected MA104 cells with strictly defined reagents we produced monoclonal antibodies against synthetic peptides from both outer capsid proteins VP4 (aa residues 228–241: QNTRNIVPVSIVSR) and VP7 (aa residues 319–326: SAAFYYRV) of simian rotavirus SA11. Two of the selected monoclonal antibodies proved to be reactive with determinants of SA11-infected MA104 rhesus monkey kidney cells, with purified SA11 as well as with the particular peptides used for immunization. The anti-VP4 antibody had a demonstrable neutralizing titer of 200 (50% focus reduction) whereas the anti-VP7 MuMAb revealed no detectable neutralizing activity. In peptide-inhibition experiments, the corresponding peptide inhibited its MuMAb whereas the noncorresponding peptide had no effect on antibody binding to intracellular viral antigen. Localization of VP7 was preceded by VP4 as shown by immunofluorescence microscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01317190
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