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  • 1
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Following one control cycle, four women were treated with 4 mg. daily of the retrosteroid Ro 6-3129 during two menstrual cycles. Judged by the plasma levels of progesterone and the urinary excretion of LH and total oestrogens, the control and treatment cycles were ovulatory.One apparently normal pregnancy and one very early spontaneous abortion occurred. There was a tendency to a reduction of the length of the follicular phase of the treatment cycles, possibly related to the medication.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: An intravenous luteinizing hormone-releasing hormone (LRH) test was performed in 287 women with amenorrhoea. Prolactin, progesterone and oestrogens in serum were also measured. Twenty-four women with premature ovarian failure and 9 with gonadal dysgenesis had raised basal follicle-stimulating hormone (FSH) levels. Neither the basal luteinizing hormone (LH) level nor the gonadotrophin responses after LRH gave a better separation of this group of women with irreversible ovarian failure. Measurement of prolactin levels were valuable in that 15 of 42 patients with hyperprolactinaemia had a radiologically abnormal pituitary fossa, whereas pituitary fossa abnormalities were found in only 11 of 245 normoprolactinaemic women. It was thought that 181 women had functional amenorrhoea; 54 per cent of these women had developed amenorrhoea in relation to weight loss and 32 per cent in relation to discontinuation of oral contraceptives. A strong correlation was found between the body weight and the basal gonadotrophin levels. The basal LH levels were correlated with serum oestrogen levels, the basal FSH level and the LH response to LRH. Most of the patients with low basal LH values had developed amenorrhoea in relation to self-imposed weight loss. The responses to LRH were often impaired in the underweight patients but became normal after weight gain. The polycystic ovary syndrome (PCO) could not be diagnosed by measuring either basal or LRH-stimulated gonatrophin levels. Single FSH and prolactin determinations in serum seemed to be the only indispensible hormone assays in the routine clinical evaluation of amenorrhoea.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Munksgaard International Publishers
    Scandinavian journal of medicine & science in sports 13 (2003), S. 0 
    ISSN: 1600-0838
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine , Sports Science
    Notes: The aim of the present study was to evaluate whether the Epo isoforms in blood, induced by short-term and intermittent hypoxia, are different from those at normoxia at sea level and if this could be an impediment to the use of a direct Epo doping test based upon the electric charge of the Epo isoforms. Ten healthy subjects, 9 men and 1 woman, participated in the study. Median age was 22 years (range 20–32). Normobaric hypoxia was administered differently in 3 sub-groups; two groups with 12 h hypoxia and 12 h normoxia up to 10 days: IM 2000 and IM 2700 living in 16.2% and 14.9% O2, corresponding to 2000 and 2700 m above sea level, respectively, and training in normoxia. The third group, C 2700, lived in hypoxia, 14.9% O2 corresponding to 2700 m, continuously for 48 h. The mean serum Epo level increased from 10.9 IUL−1 (range 8.8–12.5) to 23.5 IUL−1 (15.6–29.1) after 2 days followed by 19.7 IUL−1 (16.1–24.1) after 10 days exposure for intermittent hypoxia. The highest values 39.5 IUL−1 (31.5–50) were obtained for the group exposed for continuous hypoxia for 48 h. The median electrophoretic mobility of the serum Epo isoforms was above the cut-off limit of 670 AMU, previously estimated for discrimination between recombinant and endogenous Epo, in all samples taken before and after exposure to hypoxia. The highest values, mean 730 mAMU (range 703–750) were obtained after 10 days of intermittent hypoxia. Conclusion: If the method had been used as a doping test, no false positive results would have been registered for the 15 serum samples from the 10 individuals exposed for hypoxia. Thus, the results indicate that the basic principle for direct detection of recombinant Epo doping, based upon the change in electric charge on Epo, can be used also on individuals having lived in a hypoxic milieu.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Munksgaard International Publishers
    Scandinavian journal of medicine & science in sports 12 (2002), S. 0 
    ISSN: 1600-0838
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine , Sports Science
    Notes: The ordinary doping control urine samples of 36 anonymous participants (cross-country skiers, biathlon athletes, and curling athletes) of the 1998 Nagano Olympic Games were analyzed for erythropoietin and erythropoietin isoforms. The urine erythropoietin concentration (IU/l) was determined with a competitive radioimmunoassay method and the isoforms were studied by electrophoresis and given as milli albumin mobility units (mAMU). Erythropoietin was detectable in 23 out of 36 specimens (64%). The biathlon and curling athletes had similar urine concentration of erythropoietin. The group of 16 cross-country skiers had significantly (P 〈 0.05) increased urine concentration of erythropoietin as compared to curling athletes and four of them had urine erythropoietin concentrations between 3.6 and 5.1 IU/l. The electrophoretic mobility of erythropoietin was determined in all eight samples with urine concentration of erythropoietin of more than 2 (range 2.1–5.1) IU/l. No single urine specimen with a median erythropoietin electrophoretic mobility below the cut-off level of 670 mAMU (indicative of doping with recombinant erythropoietin) was registered. Erythropoietin in urine was detected in 71% and the isoforms of Epo characterized in 29% of the anonymous Olympic endurance athletes. The urine concentration of erythropoietin in the biathlon and curling athletes were similar to those of non-athletes. The group of cross-country skiers had higher levels of erythropoietin in urine. These higher levels of urine erythropoietin in cross-country skiers are partly due to more concentrated urine specimens.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Munksgaard International Publishers
    Scandinavian journal of medicine & science in sports 12 (2002), S. 0 
    ISSN: 1600-0838
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine , Sports Science
    Notes: Fifteen healthy males, aged 20–30 years, participated in a double-blind cross-over study on the effect of the beta-2 stimulator salbutamol on erythropoietin concentration and hemoglobin concentration. The treatment period comprised inhalation of salbutamol, 0.8 mg four times daily for 2 weeks. Serum-erythropoietin was essentially unaltered after treatment with salbutamol, 9.5±4.94 IU  ·  l−1 (mean±SD), as compared to both control situation, 9.2±4.50 IU  ·  l−1(mean±SD), and placebo treatment (9.7±4.07 IU  ·  l−1) (mean±SD). The same pattern, no significant changes between control situation and treatment with salbutamol or placebo, also applied to hemoglobin concentration. In conclusion, it is not possible to increase erythropoietin concentration and thereby erytropoiesis by inhalation of high doses of the International Olympic Committee (IOC) approved beta-2 stimulator salbutamol for 2 weeks in healthy young males.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 94 (1976), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Seventy-nine patients with acute or former reactions to penicillin were investigated by a benzyl-penicilloyl (BPO)-specific RAST and/or by skin tests with penicilloyl-polylysine (PPL), benzyl-penicillin and penicilloic acid and the results were correlated with the different clinical pictures. Positive RAST and skin test results could be found in patients with anaphylactic shock, urticaria and serum sickness-like reaction and sometimes in a special group of exanthems, which are characterized by the existence of many different lesions at the same time, therefore called ‘polymorphic exanthems’, and often observed after high-dosage penicillin therapy. In cases of scarlatiniform or morbilliform exanthems no positive results were found. The BPO-specific RAST showed an overall correlation of 95·1% with skin tests using PPL. However, some patients with positive skin tests to benzylpenicillin and penicilloic acid did not have detectable circulating IgE antibodies to BPO. This emphasizes the need for including these antigens in in vitro methods.The RAST was informative even at the time of the allergic reaction or in the first 15 days afterwards and seems to be very valuable for early diagnosis of penicillin allergy especially in cases when many drugs have been given.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of medicine & science in sports 2 (1992), S. 0 
    ISSN: 1600-0838
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine , Sports Science
    Notes: The purpose of this study was to determine resting serum erythropoietin concentrations (S-[epo]) in endurance-trained sea-level residents 2 week after arrival at moderate altitude as well as after descent to sea level, and in endurance- and strength-trained residents at moderate altitude. S-[epo] concentrations were determined in 2 groups of non-altitude-acclimatized endurance-trained athletes normally living at sea level (group UAET, 20 men and 7 women, and group UACCS, 14 men and 6 women); and 2 groups of altitude-acclimatized athletes, all long-term residents at 1900 m above sea level (strength-trained judo athletes, group AST 10 men and 11 women, and endurance-trained athletes, group AET, 10 men and 10 women). Hematocrit (Hct) but not S-[ep] was significantly higher in pup AET than group AST Resting S-[epo] in group UAET was determined l,2,7, and 14 d after arrival from sea level to 1900 m above sea level. The Hct increased significantly from day 1 to day 7 in the women of group UAET. The Hct of the males in group UAET did not change significantly throughout the 2 weeks at altitude. The women in group UAET showed a significant decrease in S-[epo] from day 1 to day 7, The S-[epo] in the men of group UAET did not change from day 1 to day 2, but decreased significantly from day 2 to day 14. Resting S-[epo] in group UACCS was obtained before and at the end of the 2-week training period at high-altitude and after high-altitude training. Only the UACCS men had, in comparison with sea-level S-[epo], similar S-[epo] after 2 weeks of high-altitude training and significantly lower S-[epo] 2 d, but not 9 or 19 d after descent to sea level. These data indicate similar resting S-[epo] levels in acclimatized endurance- and strength-trained athletes, although the former group had higher Hct than the latter. In non-altitude-acclimatized endurance athletes, S-[epo] increased the ht days at altitude and thereafter decreased to similar S-[epo] as at sea level after 2 weeks of altitude training. After descent to sea level, S-[epo] decreased transiently but returned to the pre-altitude level after 9 d
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The sera of atopic and non-atopic persons with allergic pulmonary disorders were examined for long-term sensitizing, IgE and short-term sensitizing heat-stable (S-TS) antibodies which were present separately or together in the sera of some patients sensitive to antigens such as budgerigar serum proteins and Aspergillus fumigatus.In fourteen atopic patients with extrinsic asthma, six had both types of antibody to common allergens, and of nine non-atopic patients with cryptogenic (intrinsic) asthma, four had only heat-stable short-term sensitizing antibodies. The sera of atopic subjects with type I prick test reactions and positive RAST's, showed specific IgE antibody by baboon PCA tests to budgerigar serum proteins, A. fumigatus, Timothy grass pollen extract and hen egg extract, and not to Dermatophagoides farinae, possibly because of naturally occurring mite antibodies in the baboon.The sera of non-atopic asthmatics, who had given negative prick test but positive immediate, dual or late intracutaneous tests, and only late asthmatic reactions, contained precipitins in most cases and gave little or no RAST reaction. On baboon PCA these sera contained either, S-TS antibody alone, or S-TS plus long-term sensitizing antibody, or long-term sensitizing antibody alone. Some of the sera with long-term sensitizing antibody contained blocking antibody which could diffuse away in the 24 hr delay for the baboon PCA test and could also be responsible for the negative RAST.Tests with insoluble anti-IgE immuno-adsorbents on two sera from persons sensitive to aspergillus confirmed that the S-TS activity was not due to IgE, and on two sera with negative RAST and negative prick tests to budgerigar serum antigens confirmed that the 24 hr monkey PCA responses were due to IgE.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 1 (1971), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Serological measurements of specific IgE to Dermatophagoides farinae, D. pteronyssinus and grass pollen showed statistically highly significant correlations with prick test reactions, the sizes of weals elicited, nasal tests and the clinical history. In subjects with negative prick tests, intracutaneous tests gave reactions which were not associated with specific IgE or reactions to nasal tests. Highly significant correlations were also found between total IgE values and prick test reactions to the allergens tested and to 48/80 but not to histamine; there was a negative correlation with reactions to anti-IgE serum. The reactions to prick tests identified almost all subjects with specific IgE.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The relationships of total and specific IgE levels to the clinical manifestations and atopic status of ninety-two subjects, suffering from asthma, rhinitis and infantile eczema in varying combinations, were examined. No significant correlations were found between specific IgE levels to Dermatophagoides spp. and grass pollen or between total IgE levels, and the age of the patient, nor the age of onset, duration, severity and frequency of symptoms. A highly significant correlation was found between total IgE levels and the numbers of skin test reactions to a battery of twenty routine tests and to the sum total of the graded sizes of the reactions in each subject.
    Type of Medium: Electronic Resource
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