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  • 1
    Electronic Resource
    Electronic Resource
    Incorporation of 3H-befunolol (beta-blocking agent) into melanin granules of ocular tissues in the pigmented rabbits (1981)
    Springer
    Histochemistry and cell biology 73 (1981), S. 371-375 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 3H-befunolol was administered intravenously to pigmented rabbits. Thirty minutes after the administration, the iris, ciliary body and retina were fixed, embedded and processed for light microscopic radioautography. Radioautographical silver grains were observed over the pigment granules of the iris, ciliary body, choroid and retina. From these results it is concluded that 3H-befunolol is incorporated into the pigment granules of these cells. The mechanism of incorporation is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495651
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Periodic acid — Schiff — Alcian Blue: A method for the differential staining of glycoproteins (1987)
    Springer
    Journal of molecular histology 19 (1987), S. 565-571 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The staining mechanism underlying the periodic acid—Schiff (PAS)—Alcian Blue (AB) sequence has been investigated using a variety of glycoprotein-containing tissues from different organs of the monkey, rat and mouse. The results obtained suggest that reactive carbohydrates contain at least three types of chemical end-groups found in neutral and acidic glycoproteins: (1) PA-engendered aldehyde groups coloured magenta by the Schiff reagent; (2) PA-engendered aldehyde groups coloured blue bisulphite-AB; and (3) naturally occurring acidic (carboxyl and/or sulphate) groups coloured blue by AB only. The PAS—AB sequence showed heterogeneity of glycoprotein structures in the conjunctiva and the duodenal goblet cells. Thus, the PAS—AB sequence is not the simple reverse sequence of AB—PAS but has its own definite and unique staining selectivity and can hence be used as a reliable method for the histochemistry of glycoproteins at the light microscope level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01687364
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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