ISSN:
1860-1499
Keywords:
Congenital hydrocephalus
;
Antipregnant rat kidney serum
;
p53
;
Immunohistochemistry
;
Electron microscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Congenital hydrocephalus was induced by intraperitoneal administration of antipregnant rat kidney serum (APRKS) into rats at 9 days of pregnancy. To clarify a correlation between the oncogenes and expression of hydrocephalus, immunostaining of the fetal rat nervous system for p53 was carried out using Ab-1, a pantropic antirat p53 antibody, and Ab-3, raised against a mutant form of rat p53, Ab-1 staining was observed in cells of the neural plate of the fetus, the yolk sac, and the placenta. Because no staining occurred with Ab-3, it was likely that the p53 induced by antiserum injection was the wild-type protein. This result indicated that p53 may represent a reparative reaction to DNA damage from the APRKS; also, when DNA is irreparably damaged, p53 could induce apoptosis by favoring transcription of a gene encoding an inducet of apoptosis. Insufficient apoptosis, on the other hand, may induce failure of fetal development, contributing in particular to congenital hydrocephalus and other central nervous system anomalies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01553779
Permalink