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  • 1
    Electronic Resource
    Electronic Resource
    Rous sarcoma virus-transformed fibroblasts and cells of monocytic origin display a peculiar dot-like organization of cytoskeletal proteins involved in microfilament-membrane interactions
    Amsterdam : Elsevier
    Experimental Cell Research 169 (1987), S. 202-214 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(87)90238-2
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Immunocytochemical distribution of extracellular matrix receptors in human osteoclasts: A β"3 integrin is colocalized with vinculin and talin in the podosomes of osteoclastoma giant cells
    Amsterdam : Elsevier
    Experimental Cell Research 182 (1989), S. 645-652 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(89)90266-8
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  • 3
    Electronic Resource
    Electronic Resource
    Osteoblast-osteoclast relationships in bone resorption: Osteoblasts enhance osteoclast activity in a serum-free co-culture system
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 179 (1991), S. 634-640 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0006-291X(91)91419-D
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  • 4
    Electronic Resource
    Electronic Resource
    Quantitative analysis on the rate of secondary bone mineralization (1972)
    Springer
    Calcified tissue international 10 (1972), S. 67-81 
    Details
    ISSN: 1432-0827
    Keywords: Mineralization ; X-Ray absorption ; Microhardness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé La méthode microradiographique-photométrique d'étude de l'absorption de rayons X, ainsi que la technique de microdureté, sont utilisées conjointement pour étudier la vitesse de minéralisation secondaire de l'os d'âge connu dans les ostéones de chiens jeunes et adultes. Les résultats des deux séries de mesures montrent une très bonne concordance. Ils montrent que la vitesse de la minéralisation secondaire a) décroit lentement et progressivement avec le temps dans chaque ostéone, b) subit peu de variations dans les divers ostéones de chaque sujet indépendemment de la vitesse de reconstruction, caractéristique de chaque région squelettique et c) décroit nettement avec l'âge de l'animal.
    Abstract: Zusammenfassung Die photometrische Methode zur Untersuchung der Mikroradiographien und die Mikrotechnik zur Beurteilung der Knochenhärte wurden beide zur Bestimmung der Geschwindigkeit der sekundären Mineralisation von Knochen bekannten Alters in den Osteonen von jungen und ausgewachsenen Hunden angewandt. Die Resultate beider Meß-Serien stimmten gut überein. Sie zeigten, daß die Geschwindigkeit der sekundären Knochenmineralisation a) mit der Zeit in jedem Osteon langsam und stetig abnimmt; b) kleinen Variationen in den verschiedenen Osteonen von jedem Tier unterliegt, unabhängig von der Neubildungsgeschwindigkeit, die für jede Skelettregion charakteristisch ist; c) mit dem Altern des Tieres beträchtlich abnimmt.
    Notes: Abstract The microradiographic-photometric method of studying the X-ray absorption, and the microhardness testing technique were concurrently applied to investigate the rate of secondary mineralization of bone of known age in the osteons of young immature and adult dogs. The results of the two series of measurement show a close agreement. They indicate that the rate of secondary mineralization, (a) slowly and progressively decreases with time in each osteon, (b) undergoes little variations in the various osteons of each subject independently of the reconstruction rate characteristic of each skeletal region, and (c) markedly decreases with the ageing of the animal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02012537
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Alendronate Reduces Adhesion of Human Osteoclast-like Cells to Bone and Bone Protein-Coated Surfaces (1998)
    Springer
    Calcified tissue international 63 (1998), S. 230-235 
    Details
    ISSN: 1432-0827
    Keywords: Key words: Alendronate—Bone proteins—Osteoclasts.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract. Bisphosphonates (BPs) are potent inhibitors of bone resorption and are therapeutically effective in disease of increased bone turnover, but their mechanism(s) of action remain to be elucidated. Using as experimental model human osteoclast-like cell lines derived from giant cell tumors of bone, extensively characterized for their osteoclast features, we investigated the adhesive properties of osteoclasts on bone slices and on different proteins of the extracellular matrix in the presence of BPs. Adhesion assays using bone slices pretreated with ALN, at the established active concentration, showed that, although the morphology of osteoclasts plated onto pretreated bone slices was not modified, the number of adherent cells was reduced by the treatment of about 50% vs. controls. The effect of ALN on the adhesion of osteoclast-like cells onto specific extracellular matrix proteins, such as bone sialoprotein-derived peptide, containing the RGD sequence, conjugated to BSA (BSP-BSA) and fibronectin (FN), was also tested. In the case of FN the treatment with ALN of protein-coated wells did not modify the percentage of cell adhesion compared with the control, whereas onto BSP-BSA the presence of ALN significantly reduced adhesion of about 40–45%, suggesting that the inhibitory effect of ALN on cell adhesion could probably be due to the interference with receptors specifically recognizing bone matrix proteins as αVβ3 integrins. Furthermore, ALN induced Ca-mediated intracellular signals in osteoclasts, triggering a 2-fold increase in intracellular calcium concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002239900519
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  • 6
    Electronic Resource
    Electronic Resource
    The osteoclasts of hen medullary bone under hypocalcaemic conditions (1981)
    Springer
    Anatomy and embryology 162 (1981), S. 379-392 
    Details
    ISSN: 1432-0568
    Keywords: Osteoclasts ; Medullary bone ; Bone cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Osteoclasts of medullary bone after several days of hypocalcaemic diet are substituted on the trabecular surface by active osteoblasts. The fate and the ultrastructure of the osteoclasts withdrawn from medullary bone surfaces in the course of a low calcium diet has been studied in serial semithin and ultrathin sections. The cytoplasmic surface of osteoclasts located in marrow compartments presents blebs and protrusions and the whole cell is often irregularly branched in several directions. A large amount of granular endoplasmic reticulum is accumulated at the cell periphery; often the cisternae are distended to form vesicles with an inner core of dense material. Osteoclasts seem to divide into mono or polynucleated smaller units.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00301864
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  • 7
    Electronic Resource
    Electronic Resource
    Influence of calcium depletion on medullary bone of laying hens (1980)
    Springer
    Calcified tissue international 32 (1980), S. 221-228 
    Details
    ISSN: 1432-0827
    Keywords: Medullary bone ; Calcification ; Low-calcium diet ; Parathyroid hormone ; Estrogens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Medullary bone of birds maintained on a low-calcium diet represents a good model to study modifications of matrix composition in calcified tissue undergoing intense formation and resorption. The composition of the bone matrix during the low-calcium diet has been analyzed by both chemical and histological techniques. Sixty White Leghorn pullets 1 year old were used for the experiment. Fifteen birds served as controls and were killed on day zero; the remaining birds were placed on a calcium-deficient diet (0.13% calcium) and sacrificed after 4, 7, and 12 days of treatment in groups of 15. Serum levels of calcium, PTH, and estrogens were also measured. Chemical analysis of the samples were made for total nitrogen, hydroxyproline, hexosamine, hexoses, calcium, and phosphorus. Collagen and proteoglycans of the matrix of medullary bone of the egg-laying hens were found to be affected by the low-calcium diet. They either increased or decreased during the experiment but never in parallel. The increment of serum PTH is considered responsible for the variations in the amount of collagen. The effects of this hormone are magnified by the fall of serum estrogens as shown also by variations in the amounts of noncollagenous protein. In the late phase of the diet the matrix is represented by poorly calcified osteoid tissue rich in noncollagenous protein, i.e., proteoglycans and glycoproteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408545
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  • 8
    Electronic Resource
    Electronic Resource
    Osteobiology, Strain, and Microgravity: Part I. Studies at the Cellular Level (2000)
    Springer
    Calcified tissue international 67 (2000), S. 2-9 
    Details
    ISSN: 1432-0827
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s00223001088
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  • 9
    Electronic Resource
    Electronic Resource
    Expression of estrogen receptor-alpha in cells of the osteoclastic lineage (1999)
    Springer
    Histochemistry and cell biology 111 (1999), S. 125-133 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Estrogen deficiency at the menopause is associated with an increased rate of bone loss and subsequent risk of skeletal fracture. Whilst cells of the osteoblastic lineage are known to express estrogen receptors, the presence of estrogen receptors in osteoclasts remains controversial. We have examined expression of the classic estrogen receptor, estrogen receptor-alpha (ERα), during osteoclast differentiation. In situ mRNA hybridisation with a digoxygenin-labelled riboprobe to ERα mRNA, together with immunocytochemical analysis using a human ERα-specific monoclonal antibody demonstrated similar findings and confirmed the expression of ERα in chondroblasts and osteoblasts from human fetal bone and mineralising human bone marrow cultures. ERα expression was detected in human bone marrow cultures treated with 1,25(OH)2D3 and macrophage colony-stimulating factor and in macrophage cultures treated with 1,25(OH)2D3. However, in an in vitro model of human osteoclast formation, no ERα expression was observed in the osteoclasts that developed. The human preosteoclast TCG 51 cell line showed strong expression of ERα in contrast to the low levels observed in the more mature bone resorptive TCG 23 cell line. No expression was detectable in osteoclasts cultured from giant cell tumour of bone (GCTB) tissue or in osteoclasts in Pagetic, GCTB, or hyperparathyroid bone tissues. In conclusion, preosteoclasts express detectable levels of ERα, but osteoclast maturation and bone resorption is associated with loss of ERα expression. This indicates that ERα expression and regulation may play a role in osteoclast formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050342
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  • 10
    Electronic Resource
    Electronic Resource
    Binding of osteopontin to the osteoclast integrin αvβ3 (1993)
    Springer
    Osteoporosis international 3 (1993), S. 132-135 
    Details
    ISSN: 1433-2965
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Occupancy of the chicken osteoclast αvβ3 integrin stimulates immediate cell signals. Peptides from osteopontin containing Arg-Gly-Asp and peptides from the osteopontin and bone sialoprotein sequences containing Arg-Gly-Asp stimulated immediate reductions in osteoclast cytosolic Ca2+. The changes in cytosolic Ca2+ required the Arg-Gly-Asp sequence, and were blocked by LM609, a monoclonal antibody to the αvβ3 integrin. Osteoclast stimulation by the proteins through the integrin did not require immobilization since soluble peptides produced changes in cytosolic Ca2+ and inhibited osteoclast binding to bone particles and bone resorption. The decrease in cytosolic Ca2+ stimulated by osteopontin and related peptides was due to activation of a plasma membrane Ca2+-ATPase. Thus, the data suggest that ligand binding to the osteoclast αvβ3 integrin results in a reduction in cytosolic Ca2+ which participates in regulation of osteoclast function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01621887
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