Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Expression of ODF and ICAM-1 of Bone Marrow Mesenchymal Stem Cells is Enhanced with Osteogenic Differentiation (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 361-363 (Nov. 2007), p. 1173-1176 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Osteoblasts were perceived as pivotal cells, recognized as the cells that control both the formativeand the resorptive phases of the bone remodeling cycle. Osteoblasts were an essential requirement forosteoclastogenesis though expressing or secreating bioactive osteoclast-differentiation-regulatory proteins,osteoclast differentiation factor (ODF)was the most important factor among these, ODF participate nearly inevery step of differentiation and activation of osteoclasts. In addition, intercellular adhesion molecule-1(ICAM-1)and its receptors LFA-1 play a role in osteoclast development by affecting adhesion betweenstromal cells and osteoclast progenitors before the occurrence of ODF-ODF receptor signaling. However, itis not clear about the relationship between ODF, ICAM-1 expression of osteoblasts and differentiation stateof osteoblasts. So,the aim of this study was to investgate whether the expression of ODF, ICAM-1 dependedon the stage of osteoblastic differentiation from rat bone marrow mesenchymal stem cells(rBMSCs). Theviability of rBMSCs is reduced significantly by osteogenic inducement as differentiating into osteoblasts,ALPase activity of OS-treated rBMSCs was enhanced obviously within 9 days , declined subsequently andrecovered nearly the original level at day 14. Expression of ODF is enhanced with osteogenic differentiationguadully. whereas, expression of ICAM-1 is activated at OS-treated day 6, then keeping at a stable level.This study indicated that rBMSCs undergoing osteogenic inducement was an ideal model for studying thedifferentiation and maturation of osteoblasts. During the early stage of differentiation along osteoblasts fromstem cells to osteocytes, rBMSCs or Osteoprogenitor react somewhat differently from osteoblasts,suggesting the ability of osteoblasts to regulating differentiation and maturation of osteoclasts have beenimproved with osteogenic culture
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.1173.pdf
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Effect of a Single Period of Mechanical Strain on Gene Expression Patterns of Ets1 and Cbfa1 in Murine Calvarial Sutural Osteoblast-Like Cells (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 1105-1108 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Cranial sutures produce new bone at the sutural edges of the bone fronts in response toexternal stimuli. Little is known regarding the mechanism of osteogenesis in cranial sutures. Ets1and Cbfa1 are two important osteogenic transcription factors regulating the differentiation andmaturation of osteoblasts. But their function in cranial sutures is not still elucidated. We haveinvestigated the gene expression of Ets1 and Cbfa1 in rat’s calvarial sutural osteoblast-like cellsunder a single period of mechanical strain. The cells were isolated from the cranial suture of SD ratsand cultured in vitro, and subjected to a single 40 minutes mechanical strain using a four-pointbending apparatus. The gene expression patterns of Ets1 and Cbfa1 were examined by RT-PCR.Both mRNA levels of Ets1 and Cbfa1 have increased significantly within 6 and 12 hoursrespectively after mechanical strain were applied, and the increase returned to control levelthereafter. However, Ets1 and Cbfa1 exhibited different temporal expression patterns: Ets1expressed immediately after the mechanical loading and reached the maximum transcription at0.5h; whereas Cbfa1 experienced a latency period first, then increased slowly within 2 hours, andreached the maximum transcription at 6 h. The maximum transcription of Cbfa1 was about 2.58fold of that of Ets1. Ets1and Cbfa1 may play different roles in regulating bone matrix proteinexpressions in osteoblast-like cells during suture distraction and their function is time-dependent.High frequency distraction (〉2times/24h) is favourable to the maximal expression of the two genes
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.1105.pdf
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Proliferation Effect of Mandibular Condylar Chondrocytes Given The Static Tension-Stress and/or TGF-β1 In Vitro (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 1125-1128 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The purpose of this study is to study the proliferous effect of mandibular condylarchondrocytes given static tension-stress and/or transforming growth factor-β1 (TGF-β1) in vitro. Thefourth-passage condylar chondrocytes were harvested for this study, and a pulsatile cellularmechanical system was used to apply stress on cells. The proliferous effect of condylarchondrocytes given continuous static tension-stress and/or TGF-β1 were examined by using flowcytometry. The experiment was divided into two parts. The first part was divided into 20 groupsaccording to different TGF-β1 dosage (0ng/ml, 0.1ng/ml, 1ng/ml and 10ng/ml) for 0, 6, 12, 18 and24 hours respectively. The second part was divided into eight groups under continuous statictension-stress (0 or 5kPa) and different TGF-β1 dosage (0ng/ml, 0.1ng/ml, 1ng/ml, 10ng/ml) for 12hours. Experimental data was analyzed with repeated interclass analysis of variance The resultsshowed that chondrocytes which were cultured under different TGF-β1dose combined with 5kPastatic tension-stress had multi-horn morphological characters, including a great quantity ofchondrocytes with division growth.TGF-β1 had a mitogenic effect on rat mandibular condylechondrocytes at the concentrations of 0.1 , 1 and 10ng/ml , and the mitogenic effect of TGF-β1 tocondylar chondrocytes were demonstrated after 12 to 18 hours, and the peak of mitogenic effectsappeared at the 18th hour (P 〈0.05) . The most active mitogenesis happened in the group whosechondrocytes was under continuous static tension-stress (5kPa) combined with TGF-β1. Theseresults proved that mechanical stimulus and TGF-β1 in vitro could influence and regulate thegrowth of condylar chondrocytes
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.1125.pdf
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    The Bone Remodeling and Cellular Responses to Cranial Suture Distraction Osteogenesis in Growing Goats (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 342-343 (July 2007), p. 5-8 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The purpose of the present study was to observe the response and changes of cranialsuture to the distraction forces in growing goats and to examine the expression patterns of TGF-βand BMP during suture distraction.Twenty growing goats were divided into three groups: control(n=4), experimental (n=12), and sham (n=4). A pure titanium distractor was placed in the coronalsuture in both the sham and experimental groups. After healing, the distractor was activated fordistraction of the coronal suture at a rate of 0.5 mm/day for 8 days in the experimental group. Threeanimals were killed respectively, at 0，2，4 and 8 weeks after completion of suture distraction. Noforce was applied in the sham group. X- Ray examination was taken and the coronal suture sampleswere harvested and processed for histological analysis and scanning electron microscopic analysisand immunohistochemistry of TGF-β and BMP. The coronal sutures of experimental group wereseparated successfully. Signs of intramembranous bone formation and remodeling were found in thedistracted suture，and the sutural structure almost return to its normal state at 8 weeks after end ofdistraction. At 0 and 2 weeks after completion of suture distraction, the collagen fiber bundles werestrengthened and aligned in the direction of the distracted forces. Strong expression of BMP andTGF-β were detected in the fibroblast-like cells and the active osteoblasts. At 4 weeks after suturedistraction, signs of intramembranous ossification were found in the edge areas of the distractedsuture, and the positive staining of BMP and TGF-β was still noted in the osteoblasts around thenewly formed bone trabeculae. This study suggests that cranial suture expansion can be achieved ingrowing animal by distraction osteogenesis. Mechanical strain resulted from distractor can inducethe adaptive remodeling in the cranial suture of growing goats. It also suggests BMP and TGF-βmay play very important roles in the process of bone formation and remodeling during suturedistraction osteogenesis
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.5.pdf
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    A New Four-Point Bending System to Supply Cultured Adhensive Cells In Vitro with Cyclic Uniaxial Strain (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 1133-1136 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: We have designed a new four-point bending system by combination of integrate circuit andbeam-deflection theory, and conducted a series of experiments concerning cell morphology andproliferation. The system was proved to be able to supply the cultured anchorage-dependent cells withcyclic uniaxial stretching strain and compressive stress easily, precisely and effectively
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.1133.pdf
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Effects of Mechanical Strain on Osteoblastic Precursor Cells in a Three-Dimensional Scaffold (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 1181-1184 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: It was well recognized that mechanical strain plays a crucial role in periodontal tissuesremodeling. The aim of this study was to investigate the effect of mechanical strain on osteoblasticprecursor cells in a collagen type I gel scaffold. Rat MSCs were isolated and cultured according tothe established method. Cells were induced with osteogenic medium, then seeded in a collagen typeI gel and mechanically stretched by application of cyclic biaxial strain 24h later. Strain cycle was setto 1 cycle/min (0.017Hz), and strain magnitude was set to 2%, 5%, 7% elongation. Cells werecollected in 0h, 3h, 6h, 9h, 12h, 24h and 48h respectively. ODF and ICAM-1 mRNA were analyzedby RT-PCR assay. The results shown that 2-7% elongation strain, either dynamic or static, inhibitedICAM-1and ODF expression of osteoblastic precursors, and the effects were relative tightly to strainmagnitude. The inhibition effects of dynamic strain loading group exceeded the corresponding staticstrain. This work suggested that appropriate mechanical strech may suppress differentiation ofosteoclasts through inhibiting expression of ICAM-1 and ODF. Application of mechanical stressmight have a beneficial effect on quantity of generated bone tissue and might be a important factorin tissue engineering of periodontal tissues
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.1181.pdf
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...