ISSN:
1573-6784
Keywords:
expanded-bed chromatography
;
Pichia pastoris
;
recombinant protein purification
;
wheat LTP
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Pichia pastoris produced large quantities of a wheat 9 kDa lipid transfer protein as a recombinant secreted protein. A single step procedure applying the yeast culture directly to expanded-bed Streamline SP-XL was used to purify it 4 times. From 700 ml of culture, 476 mg of highly purified LTP were obtained in one day. This represents a 6-fold higher yield in comparison to the five days purification scheme it replaces.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008953326639
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