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  • 1
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The effects of the mycoparasites Coniothyrium minitans and Trichoderma atroviride on the suppression of alfalfa blossom blight caused by Sclerotinia sclerotiorum were evaluated under indoor and field conditions. When T. atroviride (9·0 × 104 conidia/floret) + S. sclerotiorum (6·0 × 103 ascospores/floret) or C. minitans (9·0 × 104 conidia/floret) + S. sclerotiorum (6·0 × 103 ascospores/floret) were applied to detached young alfalfa florets, T. atroviride effectively inhibited saprophytic growth of S. sclerotiorum, whereas C. minitans showed no inhibition under the same conditions. When T. atroviride (6·9 × 104 conidia/floret) + S. sclerotiorum (6·0 × 103 ascospores/floret) or C. minitans (6·9 × 104 conidia/floret) + S. sclerotiorum (6·0 × 103 ascospores/floret) was applied to young alfalfa petals in vivo just after pollination, the percentage of pod formation was higher for T. atroviride+S. sclerotiorum than that for C. minitans+S. sclerotiorum, and the percentage of pod rot was lower for T. atroviride+S. sclerotiorum than that for C. minitans+S. sclerotiorum. However, when they were applied to senescent petals attached to developing pods of alfalfa at 9·2 × 104 conidia/floret together with S. sclerotiorum at 4·5 × 103 ascospores/floret at 14 days after pollination, C. minitans was more effective than T. atroviride in suppressing sclerotinia pod rot and seed rot of alfalfa. Field experiments showed that three applications of C. minitans (5·4 × 106 conidia mL−1) or T. atroviride (5·4 × 106 conidia mL−1) at a 7-day interval to blossoms of alfalfa effectively suppressed sclerotinia pod rot in two out of three annual trials. Coniothyrium minitans effectively suppressed sclerotinia seed rot in all three years, whereas T. atroviride was not effective against seed rot in any of the trial years. The efficacy of C. minitans was not significantly different (P 〉 0·05) from benomyl (250 µg ai mL−1). This study suggests that C. minitans has potential as a biocontrol agent to control blossom blight of alfalfa caused by S. sclerotiorum.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Key words Trigonella ; Fenugreek ; NORs ; Fluorescent in situ hybridization ; Genome mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Fenugreek (Trigonella foenum-graecum L.), an annual legume crop grown mainly for seed at present, has the potential to be a high-quality forage crop in western Canada. A cytological survey of germplasm stored at the USDA germplasm center demonstrated a somatic karyotype with a chromosome number of 2n=16. Structural details of two pairs of chromosomes (numbers 1 and 2) showed secondary constrictions that were presumed to be the site of rRNA genes. Fluorescent in situ hybridization (FISH) with a heterologous rRNA gene probe from wheat was utilized to physically map their location for the first time on fenugreek chromosomes. Strong hybridization signals of apparently similar copy-number sequences were detected primarily near the centromere on the metacentric chromosome 1 and near the telomere on the acrocentric chromosome 2. Silver staining of the nucleolar organizing region provided evidence that both the sites were transcriptionally active and independently capable of forming a nucleolus. However, the site on chromosome number 2 was relatively more active than the one on chromosome number 1.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 29 (1980), S. 409-417 
    ISSN: 1573-5060
    Keywords: Hordeum vulgare L. ; barley ; salinity ; bulk populations ; composite crosses ; stress tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four highly heterogeneous populations of barley were assayed for salt stress tolerance at the time of seed germination. Three of these, Davis, Dryland and Irrigated, were derived originally from a population called Composite Cross XXI and propagated until F17 under contrasting conditions. The fourth, Composite Cross XXX-C, was of relatively recent (F5) origin. This population showed the highest germinability in nutrient solution salinated with sodium chloride. Davis was poorer in salt tolerance than CC XXX-C but better than Dryland and Irrigated. Dryland and Irrigated, propagated for 14 successive genrations under contrasting levels of soil moisture, showed no significant difference in salt stress tolerance. In several hundred random samples of lines developed from these two populations, ear row number, lemma awn texture, seed weight and yield showed non-random associations with salt tolerance. These associations might be gainfully utilized in barley breeding for salt stress tolerance.
    Type of Medium: Electronic Resource
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