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  • 1
    ISSN: 0168-1656
    Schlagwort(e): Isochrysis galbana ALII-4 ; Outdoor culture ; Polyunsaturated fatty acids ; Tubular photobioreactor
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    ISSN: 0044-8486
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 1573-5176
    Schlagwort(e): eicosapentaenoic acid ; microalga ; mixotrophic growth ; Phaeodactylum tricornutum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Mixotrophic growth of the eicosapentaenoic acid (EPA)producing diatom Phaeodactylum tricornutum UTEX640 was carried out in 1-L batch cultures under anexternal irradiance of 165 μmol photons m-2s-1 by supplementing the inorganic culture mediumwith glycerol. The effect on the growth and the fattyacid profile was studied for different initialglycerol concentrations (0–0.1 M). The optimalglycerol concentration was 0.1 M.A lag phase was observed at high glycerolconcentrations. The present study also shows thatsuccessive additions of glycerol at 0.1M concentrationand using ammonium chloride as a nitrogen sourceremarkably increased the maximum biomass concentration(16.2 g L-1) and maximum biomass productivity(61.5 mg L-1 h-1). These values wererespectively 9 and 8-fold higher than in thephotoautotrophically grown control. The level ofsaponifiable lipids in mixotrophically cultured cellswas significantly higher than in photoautotrophicallycultured cells and increased with the glycerolconcentration in the medium. The concentration ofstorage lipids, saturated and monounsaturated fattyacids, were enhanced but the EPA content did notchange significantly. The EPA content was around 2.2%of biomass dry weight. The maximum EPA yield was33.5 mg L-1 d-1 and was obtained in aculture containing 0.1 M glycerol, supplementedperiodically by ammonium chloride. This productivitywas 10-fold higher than the EPA productivity obtainedunder mixotrophic conditions.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Journal of applied phycology 12 (2000), S. 355-368 
    ISSN: 1573-5176
    Schlagwort(e): light/dark cycles ; microalgae ; Phaeodactylum tricornutum ; photobioreactors, scale-up
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The effect of the light/dark cycle frequency on theproductivity of algal culture at differentday-averaged irradiance conditions was evaluated forPhaeodactylum tricornutum grown in outdoortubular photobioreactors. The photobioreactor scale-upproblem was analyzed by establishing the frequency oflight–dark cycling of cells and ensuring that thecycle frequency remained unchanged on scale-up. Thehydrodynamics and geometry related factors wereidentified for assuring an unchanged light/dark cycle.The light/dark cycle time in two different tubularphotobioreactors was shown to be identical when thelinear culture velocity in the large scale device(U LL) and that in the small scale unit (〉U LS)were related as follows:ULL = \frac f 9/7 \alpha8/7 ULS.Here f is the scale factor (i.e., the ratio oflarge-to-small tube diameters), α is afunction of the illuminated volumes in the tworeactors, and `dark' refers to any zone of the reactorwhere the light intensity is less than the saturationvalue. The above equation was tested in continuouscultures of P. tricornutum in reactors with 0.03 mand 0.06 m diameter tubes, and over the workableculture velocity range of 0.23 to 0.50 m s-1. Thepredicted maximum realistic photobioreactor tubediameter was about 0.10 m for assuring a cultureperformance identical to that in reactors with smaller tubes.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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