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  • 1
    Electronic Resource
    Electronic Resource
    Efficient hybridization between Lycopersicon esculentum and L. peruvianum via ‘embryo rescue’ and in vitro propagation (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 115 (1996), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: For the transfer of valuable traits from wild species into the cultivated tomato, excised globular-stage embryos 13 and 15 days after pollination (DAP) were cultured in vitro. Plants were regenerated from interspecific crosses of Lycopersicon esculentum cv. ‘Early pink’×L. peruvianum PI270435, and backcrosses of L. esculentum‘Giban (JF) No. 1’× (‘Early pink’× PI270435). Somatic embryos and single cotyledons emerged on hypocotyl sections of the embryos. Five to nine plantlets per embryo were obtained by clonal propagation. The hybrid nature of the plants is confirmed by comparing hybrids and parents in their ability to regenerate shoots from leaf segments in vitro, by comparing plant morphology and characteristics and by chromosome number. This study describes an efficient ‘embryo rescue’ method, as well as somatic embryogenesis by clonal propagation. A novel and simple method for the characterization of the interspecific hybrids is also reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1996.tb00912.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    “Arabidobrassica”: Chromosomal recombination and morphogenesis in asymmetric intergeneric hybrid cells (1981)
    Springer
    Planta 153 (1981), S. 586-593 
    Details
    ISSN: 1432-2048
    Keywords: Arabidopsis ; Brassica ; Chromosome elimination ; Protoplast fusion ; Somatic hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A somatic hybrid cell line, cloned from an individual protoplast-fusion product between Arabidopsis thaliana and Brassica campestris, gave rise to formation of numerous plants differing drastically in morphology. Analysis of these various regenerants, all of which originated from one and the same heterokaryon derived from the fusion of two cells, shows the unspecific elimination of chromosomes of both parental species during the callus growth phase. Whereas the parental cells have so far not been sucessfully regenerated into plants, several of their different asymmetric hybrids are capable of morphogenesis. Furthermore, chromosomal analysis indicates extensive recombination. Most of the plants are predoinantly morphologically regular. Abnormalities are mostly limited to the flowers which tend to undergo phyllody. The results demonstrate that remote somatic hybridization may have applications although true amphidiploids may not be obtainable. The transfer of small units of genetic material between distantly related species by protoplast fusion seems to be a more realistic approach than the combination of complete, highly diverse genomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385546
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Light-inducible pigmentation in Portulaca callus; selection of a high betalain producing cell line (1991)
    Springer
    Plant cell reports 10 (1991), S. 304-307 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have established a unique betalain pigmentation system in callus cultures that originated from seedlings of Portulaca sp. ‘Jewel’. Within three different ‘Jewel’ lines examined, one line (JR) was clearly superior with regard to callus growth rate and pigment formation. Furthermore, after ten cycles of selection of deeply colored callus patches, the selected clones contained on an average four times the amount of betalain as compared to the non-selected mother line. The colorization was induced by light, but disappeared in the dark. Pigment synthesis was detectable within 30 h after irradiation and showed positive correlation with irradiation periods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193147
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Expansion of the IR in the chloroplast genomes of buckwheat species is due to incorporation of an SSC sequence that could be mediated by an inversion (1997)
    Springer
    Current genetics 31 (1997), S. 276-279 
    Details
    ISSN: 1432-0983
    Keywords: Key words Buckwheat ; Chloroplast DNA ; Inversion ; Inverted repeats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chloroplast genomes in buckwheat species contain large inverted repeats which are at least 4 kbp longer than the majority of those in land plants. The length of the buckwheat inverted repeats was attributable to an additional region located adjacent to the borders of the small single-copy region. We have cloned and sequenced a 5.2-kbp SmaI fragment corresponding to this extra region in the inverted repeats. A homology search revealed that the sequence of the SmaI fragment is highly homologous to one side of the small single-copy region of the inverted repeats in dicot chloroplast DNAs such as tobacco and beechdrops. Interestingly, a 3.7-kbp segment in the middle of the SmaI fragment is inserted in the opposite orientation relative to those of the other dicot species, and 17-bp direct repeats are found located at both the ends of the additional region. These results suggest that expansion of the inverted repeats in buckwheat chloroplast DNA might have been associated with an inversion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050206
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Evidence that blue light induces betalain pigmentation in Portulaca callus (1995)
    Springer
    Plant cell, tissue and organ culture 43 (1995), S. 67-70 
    Details
    ISSN: 1573-5044
    Keywords: betalain pigment ; blue light ; callus ; Portulaca ; UV
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The wavelength range that activates betalain pigmentation has been studied following selection of light induelble betalain producing callus lines originating from Portulaca sp. ‘Jewel’ seedlings. Light sources with different wave-lengths were used to irradiate the callus, resulting in blue light being effective in inducing betalain pigmentation. In addition, when UV light was combined with blue light, some calluses from this cell line showed high production of the pigment. This is a first report that betalain pigmentation in callus was induced by blue and blue/UV lights.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042673
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Comparative analysis of petal proteins in red and white lines from near-isogenic Portulaca sp. ‘Jewel’ plants (1992)
    Springer
    Euphytica 61 (1992), S. 67-71 
    Details
    ISSN: 1573-5060
    Keywords: betalain pigment ; comparative analysis ; near-isogenic lines ; petal protein ; pigmentation locus ; Portulaca ‘Jewel’
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A couple of near-isogenic lines, JR and JW from Portulaca sp. ‘Jewel’ has red and white petals respectively. The difference in petal color in the two lines is caused by an alteration of single genetic locus which is involved in the biosynthesis of betalain pigmentation. In order to detect this genetic difference at the polypeptide level, we have now attempted the comparative analysis of petal proteins between JR and JW. The protein profiles of SDS-polyacrylamid gel electrophoresis from three different stages of the developing petals, and the autoradiographic protein profiles of in vivo labeled petals howed that a 27 KD fragment strongly appeared as a characteristic of JR petal fraction. Thus, this fragment can be considered as a candidate for certain polypeptides which is associated with the betalain synthesis pathway functioning in the pigmented petals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035548
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    Paper (German National Licenses)
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