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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 107 (1993), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Computerised image analysis was utilised to enumerate the attachment of Staphylococcus epidermidis to HEp2 cell monolayers. A differential staining technique was employed such that individual staphylococcal cells stood out in sharp contrast against the uneven cell surface and granular contents of the epithelial cells. The primary image analysis operation involved subtracting an out-of-focus image from an in-focus image of the bacteria on the monolayer, thereby accentuating the bacterial image. Enumeration, using a particle counting routine, was rapid and reproducible, facilitating counting in excess of 700 bacteria per field at ×500 magnification. The computerised programme compared favourably with manual counting and would provide a rapid, objective and morphologically discriminatory method for evaluating bacterial attachment to various tissues.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 435 (2005), S. 736-736 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Sir Your News Feature “CSI: cell biology” (Nature 434, 952–953; 2005) raises questions about the legitimacy of editing images. In this context, I am regularly surprised at the essentially binary ...
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Particle and Particle Systems Characterization 10 (1993), S. 308-312 
    ISSN: 0934-0866
    Keywords: Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper describes preliminary work carried out on the applicability of fractal geometry for differentiating between wear and contaminant particles found in mining machinery lubrication systems.Coal dust, stone dust and roadstone dust from a granite quarry were used as the contaminant particles and the particles from two crushers, which had failed whilst in service, were used to provide the wear particles.Two differing populations appeared with the wear particles having higher boundary fractals than the contaminant, but tending to spread across a wide range of fractal numbers, whereas the contaminants were lower and fairly tightly grouped.The underfractal distributions of the two populations of particles, generate straight lines when plotted on Gaussian probability paper. This leads to the probability of being able to predict the distribution of particles, in fractal terms between wear and contaminant particles.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Particle and Particle Systems Characterization 11 (1994), S. 418-425 
    ISSN: 0934-0866
    Keywords: Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Problems with automated techniques for measuring boundary fractal dimensions using structured walks are described and a new method is proposed, coordinate averaging. The actual polygon generated by a structured walk is variable and depends upon the chosen starting point. This noise is apparent in the resulting Richardson plots. The use of multiple starting points enhances the Fast (equipaced) method but is less productive with the Hybrid (fixed step) method because the paths followed around the perimeter tend to converge. Coordinate averaging uses perimeters that have been mapped as a list of sequential coordinates. They are then examined by averaging every coordinate with an increasing number of neighbours. The resulting family of shapes progressively shed detail and can be used to generate fractal dimensions. Coordinate averaging appears to be free from the noise found with structured walks and is able to examine highly convoluted shapes. In addition it appears well suited to the examination of the homogeneity of boundaries and can follow the detailed evolution of individual features.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Particle and Particle Systems Characterization 12 (1995), S. 28-37 
    ISSN: 0934-0866
    Keywords: Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method is described for mapping the perimeter of shapes as a sequence of connected X & Y coordinates. It operates by tracking, in tandem, along a dilated outline of the shape and the true outline, maintaining contact between both tracks. This combination permits the complete mapping of binary shapes regardless of their complexity. The resulting sequence of X & Y coordinates form an 8-connected map of the outline, which can then be used to obtain fractal dimensions using structured walks. The method requires a digitised image that has been converted into a binary form and the ability to read the values of individual pixels from the digitised binary image. In addition a number of approaches for increasing the efficiency of the twin tracking algorithm are outlined.Obtaining fractal dimensions from a structured walk based on a fully mapped perimeter increases the accuracy of the measured fractal dimensions, extends the range of step sizes over which measurements can be made and permits a highly detailed examination of the perimeter.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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