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Two Streptomyces lividans 66 transfer RNA genes with anticodons corresponding to serine (AGC) and arginine (CGU) codons (1993)

Ulrix, W. ; Steen, G. ; Aert, R. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

FEMS microbiology letters 109 (1993), S. 0

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ISSN: 1574-6968

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Abstract From the nucleotide sequence of a 2110-bp Streptomyces lividans 66 DNA fragment two transfer RNA genes were identified: tRNASer (AGC) and tRNAArg (CGU). These tRNA genes are transcribed from the same DNA strand and both are preceded by a putatife promoter structure. They are separated by an intergenic region of 191 bp. Like most Streptomyces tRNA genes described so far, they do not encode the 3′ terminal CCA of mature tRNAs. Both genes are followed by extensive inverted repeats, which could serve as transcriptional terminator signals. Remarkably, these hairpin structures share an identical 9 base pair stretch (5′-GAAGCCCCG-3′). Furthermore, the tRNAArg region is followed by two potential open reading frames, which are encoded on complementary strands and have 3′ overlapping ends. The gene for tRNAArg (CGU) is the first Streptomyces tRNA gene described so far which encodes the translation of a codon with uridine at the third (wobble) position.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1574-6968.1993.tb06170.x

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Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories (1997)

Jones, C.J. ; Edwards, K.J. ; Castaglione, S. ; [et al.]

Springer

Molecular breeding 3 (1997), S. 381-390

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ISSN: 1572-9788

Keywords: DNA markers ; RAPD ; AFLP ; SSR ; microsatellite ; network ; reproducibility

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract A number of PCR-based techniques can be used to detect polymorphisms in plants. For their wide-scale usage in germplasm characterisation and breeding it is important that these marker technologies can be exchanged between laboratories, which in turn requires that they can be standardised to yield reproducible results, so that direct collation and comparison of the data are possible. This article describes a network experiment involving several European laboratories, in which the reproducibility of three popular molecular marker techniques was examined: random-amplified fragment length polymorphism (RAPD), amplified fragment length polymorphism (AFLP) and sequence-tagged microsatellites (SSR). For each technique, an optimal system was chosen, which had been standardised and routinely used by one laboratory. This system (genetic screening package) was distributed to different participating laboratories in the network and the results obtained compared with those of the original sender. Different experiences were gained in this exchange experiment with the different techniques. RAPDs proved difficult to reproduce. For AFLPs, a single-band difference was observed in one track, whilst SSR alleles were amplified by all laboratories, but small differences in their sizing were obtained.