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  • 1
    Electronic Resource
    Electronic Resource
    Identification of G-proteins in rat parotid gland plasma membranes and granule membranes: presence of distinct components in granule membranes (1992)
    Springer
    Molecular and cellular biochemistry 115 (1992), S. 155-162 
    Details
    ISSN: 1573-4919
    Keywords: G-proteins ; parotid gland ; exocytosis ; granule membranes ; ADP ribosylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract We have identified by immunoblotting and ADP-ribosylation by cholera toxin and pertussis toxin the presence of Mr 43 and 46 KDa Gsα, and 39 and 41 KDa Giα;.. subunits in rat parotid gland plasma membranes but not in granule membranes. A Mr 28 KDa polypeptide that served as substrate for ADP-ribosylation by both cholera toxin and pertussis toxin was present exclusively in granule membranes. Photoaffinity crosslinking of [α-32P]GTP showed the presence of high molecular weight GTP-binding proteins (Mr 160,100 KDa) in granule membranes. Six low molecular weight GTP-binding proteins (Mr 21–28 KDa) were differentially distributed in both plasma membranes and granule membranes. The present study identifies various GTP-binding proteins in rat parotid gland plasma membranes and granule membranes, and demonstrates the presence of distinct molecular weight GTP-binding proteins in granule membranes. These granule-associated GTP-binding proteins may be involved in secretory processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230326
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  • 2
    Electronic Resource
    Electronic Resource
    A Novel Thromboxane Synthetase Inhibitor, Dp-1904, Inhibits Human Blood Eosinophil Degranulation (1997)
    Springer
    Inflammation 21 (1997), S. 1-8 
    Details
    ISSN: 1573-2576
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Eosinophils have been recognized to be associated with various immune responses and disease processes including bronchial asthma. Eosinophils release a number of cytotoxic and neurotoxic mediators. However, the factors regulating such release and the underlying mechanisms are unclear. In this study, we investigated the effect of a selective and potent thromboxane synthase inhibitor, DP-1904, on the release of eosinophil cationic protein (ECP) in platelet activating factor (PAF) and IgG-stimulated human blood eosinophils. PAF (1 μM) and IgG both released ECP which constituted about 25–30% of the total ECP content. The control protein, ovalbumin, did not release any ECP over the basal values. DP-1904 in two different concentrations, 10 μM and 100 μM, significantly attenuated the release of ECP in response to PAF or IgG. The mean percent inhibition by 10 μM DP-1904 was 49 ± 10 and 31 ± 2 against PAF and IgG-induced ECP release, respectively. However, at 100 μM DP-1904 the percent inhibition was 76 ± 14 and 67 ± 2, respectively. These data suggest that TXA2 is an important mediator in the regulation of eosinophil degranulation, and DP-1904 thus might prove beneficial in the treatment of bronchial asthma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1027330506697
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  • 3
    Electronic Resource
    Electronic Resource
    Endothelin-1 induces tyrosine phosphorylation in human blood monocytes (1996)
    Springer
    Molecular and cellular biochemistry 159 (1996), S. 33-38 
    Details
    ISSN: 1573-4919
    Keywords: protein tyrosine kinase ; monocytes ; endothelin-1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Mediators including the neuropeptide endothelin-1 (ET-1), which are released in response to injury, modulate the expression of cell adhesion molecules on leukocytes and endothelial cells. The mechanisms underlying this process are not clear. In this study we investigated the effect of endothelin-1 on the expression of tyrosine phosphorylated proteins in human blood monocytes. Endothelin-1 caused an increase in tyrosine phosphorylated proteins in monocytes in a time-dependent and dose-dependent manner, the Mr 60, 80 and 110 kDa proteins being the most prominent. This effect was blocked by pre-incubating the monocytes with the selective tyrosine kinase inhibitors genistein or herbimycin A. Endothelin-1-induced upregulation of tyrosine phosphorylated proteins appears to be mediated by the ETAreceptor. Unlike our previously reported studies in endothelial cells, immunoprecipitation with anti-src or anti-JAK antibodies followed by immunoblotting with PY20 in human blood monocytes revealed that these proteins of Mr 60, 80 and 110 kDa were not related to src or JAK kinases. These findings suggest that ET-1 exerts its effect on monocytes by a pathway involving tyrosine kinases other than src or JAK kinases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226060
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  • 4
    Electronic Resource
    Electronic Resource
    Enhanced expression of GTP-binding proteins in differentiated U937 monocytic cells: Possible involvement of tyrosine kinase and protein kinase C (1995)
    Springer
    Molecular and cellular biochemistry 152 (1995), S. 113-120 
    Details
    ISSN: 1573-4919
    Keywords: protein tyrosine kinase ; monocytes ; macrophages ; G-proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Monocytic U937 cells were differentiated into mature macrophages in the presence of 100 nM phorbol 12-myristate 13-acetate (PMA) for 24 h at 37°C. We investigated the alterations in the expression of GTP-binding proteins that take place during differentiation of these cells. A 40 KDa α-subunit of the inhibitory G-protein was identified by specific antibodies to Giα-1/2 and Giα-3 on Western blots and also by ADP-ribosylation catalyzed by pertussis toxin. The expression of the 40 KDa Giα subunit was increased 3.4 fold in differentiated cells. The expression of a 43 kDa Gsα subunit identified by Western blotting using specific antibody to Gsα and by ADP-ribosylation in the presence of cholera toxin was increased approximately 2 fold in differentiated cells. A faintly recognizable 46 KDa Gsα subunit was also increased but to a lesser extent (1.3 fold). Small molecular weight GTP-binding proteins identified by [35S]GTPγS binding on nitrocellulose blots were also increased significantly. The PMA-induced expression of Giα-1/2 and Gsα subunits was blocked to control level by both genistein and staurosporine, inhibitors of protein tyrosine kinase and protein kinase C, respectively. However, staurosporine was unable to block the PMA-induced expression of Giα-3; this was blocked only by genistein. These data suggest a role for tyrosine kinase and protein kinase C in the expression of G-proteins during differentiation of U937 cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01076073
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  • 5
    Electronic Resource
    Electronic Resource
    Identification of guanine nucleotide binding regulatory proteins in bovine tracheal smooth muscle (1996)
    Springer
    Molecular and cellular biochemistry 154 (1996), S. 179-184 
    Details
    ISSN: 1573-4919
    Keywords: G-proteins ; airway smooth muscle ; ADP-ribosylation ; immunoblotting ; photolabeling ; nitrocellulose blot overlay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The identity of G-proteins in airway smooth muscle is not well elucidated. In the present study, by immunoblotting using AS/7 antibody specific for Giα−1/2, EC/2 antibody specific for Giα−3 and RM/1 antibody specific for GSa, we identified, respectively, Mr 39, 41, 46 and 52 KDa, Mr 41 and 43 KDa, and Mr 43 and 46 KDa polypeptides of conventional (heterotrimeric) G-proteins in purified membranes of bovine tracheal smooth muscle. The identity of the Mr 41, 43 and 52 KDa Giα, and the Mr 43 and 46 Gsα was also confirmed by ADP-ribosylation with pertussis and cholera toxins, respectively. In addition, the common antibody (AG/1) for both Giα and Gsα revealed the presence of all the above polypeptides, except the Mr 52 KDa band. By nitrocellulose blot overlay with [35S]GTPγS, we also detected seven low molecular weight GTP-binding proteins of Mr 18–30 KDa in the bovine tracheal smooth muscle. Photoaffinity crosslinking of [α-32P]GTP demonstrated the presence of high molecular GTP-binding proteins of Mr 55, 75 and 110 KDa. It is concluded that plasma membranes of bovine tracheal smooth muscle contain various types of conventional, low molecular weight and high molecular weight G-proteins. This warrantes further attention to elucidate the functional roles of G-proteins in airway smooth muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226786
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  • 6
    Electronic Resource
    Electronic Resource
    Arterial embolism from anatomical variation at the thoracic outlet (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Clinical Anatomy 8 (1995), S. 222-226 
    Details
    ISSN: 0897-3806
    Keywords: arterial embolism ; developmental anomalies ; thoracic outlet syndrome ; thrombolysis ; urokinase ; Life and Medical Sciences ; Miscellaneous Medical
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Thoracic outlet syndrome (TOS) represents a constellation of symptoms arising from the compression of the neurovascular bundle as it exits the thorax. We report a unique case of multiple rare anatomical anomalies resulting in TOS manifested by distal arterial embolism. These anomalies include the combination of: (1) a unilateral right cervical rib, Gruber's type II (Gruber 1869), (2) nonunion of the first thoracic rib, (3) abnormal fibrous insertions of the anterior scalene muscle onto the epineurium of the brachial plexus and adventitia of the subclavian artery, (4) anterior position of the brachial plexus in relation to the third portion of the subclavian artery, and (5) the bifurcation of a single root of the phrenic nerve at the level of the anterior scalene muscle. This series of findings suggest an underlying developmental abnormality with a delayed onset of symptomatology consisting of the thoracic outlet syndrome. © 1995 WiIey-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ca.980080308
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  • 7
    Electronic Resource
    Electronic Resource
    Posttraumatic innominate artery aneurysm with occlusion of the common carotid artery at its origin by an intimal flap (1993)
    Springer
    Annals of vascular surgery 7 (1993), S. 368-373 
    Details
    ISSN: 1615-5947
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Blunt trauma involving the innominate and carotid arteries is a rare occurrence that can be lethal or have serious neurologic sequelae. To our knowledge this is the first reported case in the international literature describing the association of posttraumatic innominate, artery aneurysm with total occlusion and thrombosis of the common carotid artery at its origin by an intimal flap. The diagnostic problems created by this unusual injury are discussed. In this case the patency of the distal portion of the common and internal carotid arteries was demonstrated by magnetic resonance angiography (MRA), whereas color duplex and digital arteriographic studies were unsuccessful. This demonstration was crucial to patient management. Since no studies are available comparing color duplex imaging, conventional arteriography, and MRA in the evaluation of blunt carotid trauma, this case study is presented to demonstrate the utility of MRA in emergency situations. In addition, we analyze the possible pathogenesis and discuss the surgical treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02002892
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  • 8
    Electronic Resource
    Electronic Resource
    Neuropeptide Y levels during ischemia and reperfusion in the canine infrarenal aortic revascularization model (1994)
    Springer
    Annals of vascular surgery 8 (1994), S. 350-355 
    Details
    ISSN: 1615-5947
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Neuropeptide Y (NPY) is a potent vasoconstrictive polypeptide colocalized with norepinephrine in sympathetic neurons. The effects of ischemia and reperfusion on plasma NPY levels were studied and compared in the mongrel dog after infrarenal aortic cross-clamping. We found a two-to threefold increase in NPY levels during ischemia (initial 10.0 ± 1.8 pmol/L vs. maximum 24.7 ± 2.31 pmole/L,p 〈 0.001). The increase in NPY remained following reperfusion (initial 10.0 ± 0.8 pmole/L vs. maximum 23.9 ± 2.31 pmole/L,p 〈 0.001). These data reveal that NPY is released during ischemia and reperfusion and may be involved in mediating remote vascular events associated with infrarenal aortic cross-clamping.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02132996
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