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  • 1
    Electronic Resource
    Electronic Resource
    Evolution ofαq- andβ-tubulin genes as inferred by the nucleotide sequences of sea urichin cDNA clones (1983)
    Springer
    Journal of molecular evolution 19 (1983), S. 397-410 
    Details
    ISSN: 1432-1432
    Keywords: Tubulin cDNA sequences ; Silent substitutions ; Protein evolution ; 3′untranslated sequences ; Multigene families
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Evolutionary studies on the tubulin multigene families were initiated by nucleotide sequence analysis of cDNA clones complementary to sea urchin (Lytechinus pictus) tesits α- and β-tubulin mRNAS. Sequence comparisons of three partial β-tubulin cDNA clones (pβ1, pβ2, pβ3) demonstrated the existence of tubulin mRNA heterogeneity. pβ2 and pβ3 contain identical tubulin-coding regions and extremely similar 3′ untranslated sequences, including a polyadenylation signal (AAUAAA). However, pβ2 contains an additional region of 3′ untranslated sequence which includes a second plyadenylation signal. These two sequences may be allelic, representing products of alternative transcription termination or processing pathways. pβ1 and pβ2 (or pβ3) cDNAs almost certainly correspond to transcripts of distinct but evolutionarily related genes. Examination of the available coding portions showed that they differ only by a few silent nucleotide substitutions and the deletion/insertion of one codon; most of the differences are clustered within the last 15 3′-end codons. In contrst, their 3′ untranslated sequences are considerably divergent. Nucleotide alignment in this region was feasible by considering specific point and segmental mutations, mainly T↔C transitions and small deletions/insetions associated with small direct repeats. The sea urchin α- and β-tubulin cDNA and corresponding protein sequences were compared with previously described tubulin cDNA and protein sequences from other organisms. Both α and β tubulins are very conserved proteins, evolving with a rete comparable to that of histones. Analysis of the nucleotide divergence of the coding cDNA regions showed that relacement sites have changed with a rate 20–175 times lower than that of the silent sites. Among the 177 codons compared between the sea urchinb testic and chick brain β-tubulin cDNAs, there are 7 conservative amino acid replacements and the deletion/insertion of two codons. Most of these changes are clustered near the C-terminus. The 161-amino acid portion of chick brain, rat and porcine α-tubulin sequences differs by 3 conservative amino acid replacements from the corresponding sea urchin testis α-tubulin sequence. The compared interspecies 3′ untranslated sequences are very divergent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02102315
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  • 2
    Electronic Resource
    Electronic Resource
    XI. Yeast sequencing reports. The complete sequencing of a 24·6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. 663-679 
    Details
    ISSN: 0749-503X
    Keywords: Genome sequencing ; Saccharomyces cerevisiae ; chromosome XI ; catabolic threonine dehydratase ; membrane transporter ; hydantoinase ; phospholipase A2-activating protein ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We report the entire sequence of a 26·4 kb segment of chromosome XI of Saccharomyces cerevisiae. Identification of the known loci URA1, TRP3 and SAC1 revealed a translocation compared to the genetic map. Additionally, six unknown open reading frames have been identified. One of them is similar to catabolic threonine dehydratases. Another one contains characteristic features of membrane transporters. A third one is homologous in half of its length to the prokaryotic hydantoinase HyuA and in the other half to hydatoinase HyuB. A fourth one is homologous to the mammalian phospholipase A2-activating protein. A fifth one, finally, is homologous to the hypothetical open reading frame YCR007C of chromosome III. The sequence has been deposited in the EMBL data library under Accession Number X75951.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320100511
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  • 3
    Electronic Resource
    Electronic Resource
    Sequence analysis of a 40·7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 12 (1996), S. 787-797 
    Details
    ISSN: 0749-503X
    Keywords: genome sequencing ; Saccharomyces cerevisiae ; chromosome X ; VPS35 ; INO1 ; SnR128 ; SnR190 ; MP12 ; YAK1 ; RPB4 ; YUR1 ; TIF2 ; MRS3 ; URA2 ; RSP25 homologue ; leucine zippers ; membrane proteins ; glycogenin glycosyltransferase ; human phospholipase D ; chromosome XI ; gene cluster translocation/recombination ; Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The complete nucleotide sequence of a 40·7 kb segment about 130 kb from the left end of chromosome X of Saccharomyces cerevisiae was determined from two overlapping cosmids. Computer analysis of that sequence revealed the presence of the previously known genes VPS35, INO1, SnR128, SnR190, MP12, YAK1, RPB4, YUR1, TIF2, MRS3 and URA2, three previously sequenced open reading frames (ORFs) of unknown function 5′ of the INO1, 5′ of the MP12 and 3′ of the URA2 genes and 13 newly identified ORFs. One of the new ORFs is homologous to mammalian glycogenin glycosyltransferases and another has similarities to the human phospholipase D. Some others contain potential transmembrane regions or leucine zipper motifs. The existence of yeast expressed sequence tags for some of the newly identified ORFs indicates that they are transcribed. A cluster of six genes within 10 kb (YUR1, TIF2, two new ORFs, an RSP25 homologue and MRS3) have homologues arranged similarly within 28·5 kb on the right arm of chromosome XI. The sequence has been deposited in the EMBL data library under Accession Number X87371.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    XI. Yeast sequencing reports. Sequencing of a 13·2 kb segment next to the left telomere of yeast chromosome XI revealed five open reading frames and recent recombination events with the right arms of chromosomes III and V (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 10 (1994), S. S81 
    Details
    ISSN: 0749-503X
    Keywords: Genome sequencing ; Saccharomyces cerevisiae ; chromosome XI ; chromosomal recombination ; telomeres ; gene redundancy ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We report the entire sequence of a 13·2 kb segment next to the left telomere of chromosome XI of Saccharomyces cerevisiae. A 1·2 kb fragment near one end is 91% homologous to the right arm of chromosome III and 0·7 kb of that are 77% homologous to the right arm of chromosome V. Five open reading frames are included in the sequenced segment. Two of them are almost identical to the known YCR104W and YCR103C hypothetical proteins of chromosome III. A third one contains a region homologous to the Zn (2)-Cys (6) binuclear cluster pattern of fungal transcriptional activators. The fourth one, part of which is similar to the mammalian putative transporter of mevalonate, has the structure of membrane transporters. The fifth one is similar to yeast ferric reductase. The sequence has been deposited in the EMBL data library under Accession Number X75950.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320100011
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  • 5
    Electronic Resource
    Electronic Resource
    Sequence Analysis of a 33·2 kb Segment from the Left Arm of Yeast Chromosome XV Reveals Eight Known Genes and Ten New Open Reading Frames Including Homologues of ABC Transporters, Inositol Phosphatases and Human Expressed Sequence Tags (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 13 (1997), S. 583-589 
    Details
    ISSN: 0749-503X
    Keywords: genome sequencing ; Saccharomyces cerevisiae ; chromosome XV ; IRA2 ; DEC1 ; NUF2 ; HST1 ; RTG1 ; RIB2 ; HAL2 ; SCORFAC ; white gene ; ABC transporters ; inositol phoshatases ; membrane proteins ; ATP/GTP-binding ; human ESTs ; WD repeats ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The complete nucleotide sequence of a 33 221 bp segment, contained in cosmid pEOA1044, derived from the left arm of chromosome XV of Saccharomyces cerevisiae, appears in public databases between coordinates 177013 and 210234 (http://speedy.mips.biochem.mpg.de/). Computer analysis of that sequence revealed the presence of the previously known genes IRA2, DEC1, NUF2, HST1, RTG1, RIB2 and HAL2, one previously partially sequenced open reading frame (ORF) of unknown function (SCORFAC) and ten newly identified ORFs. One of the new ORFs is similar to the Drosophila melanogaster white gene and other transmembrane ABC transporters, another one has similarities to inositol phosphatases and others are similar to ORFs of unknown function from various organisms, including human Expressed Sequence Tags (ESTs). Potential transmembrane regions, ATP/GTP-binding and WD motifs have also been identified. The existence of yeast ESTs for two of the newly identified ORFs indicates that they are transcribed. © 1997 John Wiley & Sons, Ltd.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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