ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
ADP–glucose pyrophosphorylase is the key regulatory enzyme in the biosynthesis of starch in plants and glycogen in bacteria. The enzyme from potato tuber is comprised of a regulatory subunit and a catalytic subunit and is present as a heterotetramer (α2β2). The catalytic subunit from potato tuber (50 kDa) was crystallized in four different forms, two of which are suitable for structural studies. A tetragonal crystal form obtained in the presence of the substrate analog Cr-ATP diffracted to 2.2 Å and belongs to space group P41 (or its enantiomorph), with unit-cell parameters a = b = 110.57, c = 190.14 Å. A second crystal form obtained diffracted to 2.8 Å and belongs to space group P2, with unit-cell parameters a = 80.06, b = 138.84, c = 92.20 Å, β = 112.40°. As this protein displays no significant homology to any currently known protein structure, a search for heavy-atom derivatives has been initiated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444999015012
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