ISSN:
1432-0789
Keywords:
Extracellular enzymes
;
Protease activity
;
ATP content
;
Bacteria
;
Microbial biomass
;
CO2 evolution
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Geosciences
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary In an incubation experiment, soil was amended to induce changes in microbial growth and enzyme production. The soluble fraction of newly produced protease (extracellular enzyme) was separated from the soil by a sterilized millipore filter. The activity of total and soluble protease, ATP content, number of acridine orange-stained bacteria, and CO2 evolution in soils were measured during the incubation. Increases in soluble and total protease activities in soils amended with agar and glucose coincided with increases in ATP content, total counts of bacteria, growth of fungi, and CO2 evolution. In amended soils, the activity of soluble extracellular protease was about 30% of the total protease activity. Soluble extracellular protease activity was highly correlated with total protease activity (r=0.78, P〈0.01), ATP content (r=0.74, P〈0.01), and total counts of bacteria (r=0.94, P〈0.01) during the first 6 days of incubation. Hence measurement of microbial biomass appeared to be an index for the level of extracellular enzymes in soil.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00395465
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