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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 96 (1975), S. 185-204 
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. The innervation pattern of the intrinsic foregut musculature was determined using physiological methods in blue crabs (Callinectes) and spiny lobsters (Panulirus) (Tables 1, 2). The distributions of individual axons were observed in methylene blue stained preparations and their physiological effects were observed by recording excitatory postsynaptic potentials (EPSPs) in various muscles while stimulating the axons directly or while the stomatogastric ganglion was active (Figs. 4–8). 2. InCallinectes the gastric mill muscles were all singly motor innervated, while many of the pyloric muscles were multiply innervated with as many as 3 motor axons to a single muscle (Table 1). InPanulirus the pyloric muscles also showed multiple innervation with a maximum of 5 axons to a single muscle (Table 2). 3. EPSPs varied in shape and amplitude from one muscle to another in both species even when two or more muscles were innervated by the same axon (Fig. 4). InPanulirus variation of EPSPs along individual muscle fibers was observed, indicating non-uniform innervation. 4. InCallinectes the variation in size of the EPSP from one recording site to another was correlated with degree of facilitation and with sarcomere length. Fibers with small EPSPs generally showed more facilitation and relatively shorter sarcomere lengths than fibers with large EPSPs (Table 1). However, instances were found in which the usual correlation did not hold. 5. Also in the blue crab a correlation was found between resting sarcomere length and contractile properties with short sarcomered fibers giving faster contractions than longer sarcomered fibers, as in other crustacean muscles. 6. The complex variety of muscle fiber types and EPSP properties apparently serves to translate the bursts of impulses from the stomatogastric ganglion into the necessary stomach movements. 7. Comparison of the innervation patterns of the gastric mill and pyloric regions suggest that the innervation patterns may be related to the functional roles of these two regions. Multiple innervation of the pyloric region allows rapid depolarization of the muscles to be achieved with a comparatively low frequency of impulses in each axon.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 99 (1975), S. 211-230 
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. The physiological effects of two inhibitory axons supplying the stretcher muscles of three crabs (Hyas, Gecarcinus andGrapsus) were investigated, with a view to establishing the relative importance of pre- and postsynaptic inhibition. 2. InHyas, the specific inhibitory (SI) axon and the common inhibitory (CI) axon both exerted powerful presynaptic inhibition on the terminals of the excitatory axon. The effect of the SI axon was generally stronger, but some excitatory terminals were found in which CI axon stimulation was more effective. Thus, inhibition of the excitatory postsynaptic potential recorded intracellularly from the muscle fiber is the statistical result of variable inhibition at different excitatory terminals. 3. InHyas, postsynaptic inhibition was insignificant at low frequencies of stimulation, but increased at higher frequencies, for both SI and CI axons. The SI axon had a stronger postsynaptic effect than the CI axon. 4. InGecarcinus, the CI axon had a more powerful postsynaptic effect than the SI axon, whereas the SI axon was more effective at the presynaptic level. The two axons are specialized for different types of inhibition. 5. InGrapsus, the SI axon was more effective than the CI axon at both pre- and postsynaptic levels. Thus, considerable variation in pre- and postsynaptic connections of the two inhibitor axons occurs in different species of crabs. 6. In all species, the CI axon was least effective in muscle fibers with small, facilitating excitatory postsynaptic potentials. Sometimes the CI axon did not produce any effect at all in such fibers. 7. Presynaptic inhibition in crabs probably occurs mainly at narrow “bottlenecks” of the excitatory axon, where impulses are easily blocked by inhibitory synaptic action.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 627 (1991), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 627 (1991), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Aquaculture research 34 (2003), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: This study was conducted (1) to evaluate the effects of photoperiod (fixed vs. decreasing light), fish size (136 vs. 220 mm), dissolved ions (hardness and salinity) and diet (menhaden oil vs. coconut oil-based) on the tolerance (survival) of Nile tilapia, Oreochromis niloticus, to low temperatures (decreased by approximately 0.5 °C per day) and (2) to evaluate the effect of dietary fatty acid composition on selected physiological characteristics of Nile tilapia exposed to decreasing temperatures. Size significantly affected mortality, with smaller fish being less tolerant to low temperatures than larger fish. Results were equivocal in the photoperiod, dissolved ion and dietary lipid experiments, and were dependent on the method of data analysis employed. Diet significantly affected plasma osmolarity, with higher values in fish fed the menhaden-based diet. Haematocrit, serum glucose, sodium and cortisol concentrations, serum and splenic lysozyme activities, lymphocyte count and hepatosomatic index were not affected by diet. Haematocrit, white cell count and serum glucose and sodium concentrations were significantly affected by temperature, but serum and splenic lysozyme content, hepatosomatic index, and serum cortisol concentrations were not. The results of this series of experiments indicate that altering the environment or diet has little effect on the ability of Nile tilapia to survive low temperatures.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 195 (1962), S. 387-388 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The muscle used in these experiments was the abductor muscle of the first walking leg of the crayfish Astacus fluviatilis. This muscle is relatively small, consisting of a flat sheet of 30-40 muscle fibres, hence it can be quickly depolarized by adding van Harreveld's solution3 containing excess ...
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Invertebrate neuroscience 1 (1996), S. 291-307 
    ISSN: 1439-1104
    Keywords: presynaptic ; crustacean ; synapse ; plasticity ; ultrastructure ; adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Crustacean motor neurons exhibit a wide range of synaptic responses. Tonically active neurons generally produce small excitatory postsynaptic potentials (EPSPs) at low impulse frequencies, and are able to release much more transmitter as the impulse frequency increases. Phasic neurons typically generate large EPSPs in their target cells, but have less capability for frequency facilitation, and undergo synaptic depression during maintained activity. These differences depend in part upon the neuron's ongoing levels of activity; phasic neurons acquire physiological and morphological features of tonic neurons when their activity level is altered. Molecules responsible for adaptation to activity can be sought in single identified phasic neurons with current techniques. The fact that both phasic and tonic neurons innervate the same target muscle fibers is evidence for presynaptic determination of synaptic properties, but there is also evidence for postsynaptic determination of specific properties of different endings of a single neuron. The occurrence of high- and low-output endings of the same tonic motor neurons on different muscle fibers suggests a target-specific influence on synaptic properties. Structural variation of synapses on individual terminal varicosities leads to the hypothesis that individual synapses have different probabilities for release of transmitter. We hypothesize that structurally complex synapses have a higher probability for release than the less complex synapses. This provides an explanation for the larger quantal contents of ‘high-output’ terminals (where the proportion of complex synapses is higher), and also a mechanism for progressive recruitment of synapses during frequency facilitation.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 135 (1980), S. 191-199 
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Locusts,Schistocerca americana, were deprived of visual input in an attempt to alter the physiology of the high order descending contralateral movement detector (DCMD) neuron by chronically altering its activity level. 1. Locusts deprived of light for their entire nymphal development exhibited greatly reduced DCMD responses to a standard stimulus. This reduction was not reversible over a time course of several hours (Figs. 1, 2). 2. This reduction in excitability of light deprived DCMD's was accompanied by an increase in habituation to series of standard stimuli of interstimulus intervals (ISI) of 5 min and 15 s (Fig. 3). 3. Phase and/or rearing conditions affected the absolute excitability of the DCMD but did not affect habituation properties (Fig. 5). 4. Locusts placed in darkness at the age of 4 days rather than at hatching did not show the increase in habituation of DCMD responsiveness characteristic of light-deprived animals. 5. The light deprivation effect on DCMD responsiveness and habituation properties was largely reversible over 14 or more days recovery in room light (Fig. 5). 6. The electroretinogram (ERG) amplitude of light-deprived locusts was reduced as compared to control (Table 1). This reduction showed some reversibility after 14 or more days recovery in light. 7. These results are consistent with changes in the effectiveness of synaptic interactions in the optic lobes resulting from experience induced modifications in neuronal activity.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 157 (1985), S. 461-467 
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ‘fast’ axon supplying the closer muscle in crayfish (Procambarus clarkii) normally fires few impulses and generates large excitatory postsynaptic potentials (EPSPs) that fatigue rapidly with repeated stimulation. When the dactyl of one claw is immobilized in the closed position, impulse production in the fast axon decreases on the immobilized side and increases on the contralateral side. On the immobilized side, EPSPs become larger but more readily depressed with repeated stimulation, while converse changes occur on the contralateral side. In order to establish whether the smaller number of impulses on the immobilized side was responsible for the changes in EPSPs, extra impulses were generated in the fast axon of immobilized claws by implanting electrodes in the claw. Raising the impulse production to equal or exceed that of the contralateral side did not prevent the changes in EPSPs produced by immobilization. Thus, it is probable that changes in the level of synaptic input to central parts of the fast closer excitor neuron are mainly responsible for altered physiological properties of peripheral synapses, rather than the fast axon's impulse traffic per se.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1351
    Keywords: Drosophila ; Neuromuscular ; Haemolymph ; Membrane potential ; Synaptic potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Neuromuscular preparations from third instar larvae of Drosophila are not well-maintained in commonly used physiological solutions: vacuoles form in the muscle fibers, and membrane potential declines. These problems may result from the Na∶K ratio and total divalent cation content of these physiological solutions being quite different from those of haemolymph. Accordingly haemolymph-like solutions, based upon ion measurements of major cations, were developed and tested. Haemolymph-like solutions maintained the membrane potential at a relatively constant level, and prolonged the physiological life of the preparations. Synaptic transmission was well-maintained in haemolymph-like solutions, but the excitatory synaptic potentials had a slower time course and summated more effectively with repetitive stimulation, than in standard Drosophila solutions. Voltage-clamp experiments suggest that these effects are linked to more pronounced activation of muscle fiber membrane conductances in standard solutions, rather than to differences in passive muscle membrane properties or changes in postsynaptic receptor channel kinetics. Calcium dependence of transmitter release was steep in both standard and haemolymph-like solutions, but higher external calcium concentrations were required for a given level of release in haemolymph-like solutions. Thus, haemolymph-like solutions allow for prolonged, stable recording of synaptic transmission.
    Type of Medium: Electronic Resource
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