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  • 1
    Electronic Resource
    Electronic Resource
    Proteoglycan synthesis and deposition in fetal rat bone (1984)
    s.l. : American Chemical Society
    Biochemistry 23 (1984), S. 1572-1576 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00302a035
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation of three species of proteoglycan synthesized by cloned bone cells (1983)
    s.l. : American Chemical Society
    Biochemistry 22 (1983), S. 831-837 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00273a019
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Initial attachment of fibroblast-like cells to periodontally-diseased root surfaces in vitro (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of clinical periodontology 13 (1986), S. 0 
    Details
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract The precise factors mediating both initial cell attachment and longer term connective tissue reattachment after tissue destruction due to periodontal disease are not known. An in vitro model was used to assess initial attachment of fibroblast-like cells to periodontally-diseased root surfaces. Root fragments were obtained from freshly extracted teeth from 6 different patients. Individual roots were prepared such that a comparison could be made of initial attachment to non-instrumented diseased root surface, curetted diseased root surface and the non-diseased, non-instrumented portion of the same root. The amount of hard tissue removed by instrumentation was quantitated and kept constant between 0.9–1.0 mm. The unsterilized fragments were incubated with human gingival fibro-blasts (HGF) for 1 h at 37°, after which the roots were first washed to remove non-adherent cells and then photographed. The number of attached cells per unit area was quantitated from the photographs using a grid system. No significant differences could be detected between the numbers of cells attached to the 3 types of root surfaces studied on the individual roots or between any of the roots studied. Thus, initial attachment of HGF to diseased root surfaces is not inhibited by the presence of plaque or endotoxins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-051X.1986.tb00874.x
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  • 4
    Electronic Resource
    Electronic Resource
    A comparison of fibronectin and laminin binding to undemineralized and demineralized tooth root surfaces (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 21 (1986), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To determine the effect of altering the nature of the surface of the roots of teeth upon selective attachment of specific cell types, we have studied the binding of fibronectin (FN) and laminin (LN) to both undemineralized and demineralized root surfaces using a modified direct ELISA. Discs of dentine with the cementum removed were prepared from porcine premolar roots and incubated with various concentrations of FN or LN. The amount of attachment protein that binds to the dentine surface was determined using specific antibodies. It was found that FN binding was two-fold greater on demineralized compared to undemineralized root surfaces. Healing the demineralized discs increased FN binding, whereas bacterial collagenase treatment essentially abolished binding, suggesting that FN binding occurred to exposed collagen fibres. In contrast, FN binding on undemineralized discs was unaffected by heat or collagenase treatment. Pronase treatment eliminated FN binding to demineralized and to undemineralized discs, whereas 4M guanidine hydrochloride extraction increased binding lo undemineralized discs, indicating that a non-collagenous protein on Ihe undemineralized surface may bind to FN, Studies with LN showed a two-fold greater binding of LN to undemineralized compared to the demineralized roots. These results indicate that surface treatment of exposed roots may be used to promote the adhesion of specific attachment proteins and thereby promote selective attachment of cells to tooth root surfaces.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1986.tb01435.x
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  • 5
    Electronic Resource
    Electronic Resource
    Genome-wide analysis of mouse transcripts using exon microarrays and factor graphs (2005)
    [s.l.] : Nature Publishing Group
    Nature genetics 37 (2005), S. 991-996 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Recent mammalian microarray experiments detected widespread transcription and indicated that there may be many undiscovered multiple-exon protein-coding genes. To explore this possibility, we labeled cDNA from unamplified, polyadenylation-selected RNA samples from 37 mouse tissues to microarrays ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng1630
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  • 6
    Electronic Resource
    Electronic Resource
    Microfilament rearrangements during fibroblast-induced contraction of three-dimensional hydrated collagen gels (1984)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 4 (1984), S. 29-40 
    Details
    ISSN: 0886-1544
    Keywords: microfilaments ; microtubules ; contraction ; collagen gel ; fibroblasts ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In vitro models have been developed recently to study the ability of fibroblasts to generate tensile force within collagen gels. The present study was initiated to assess the role of the cytoskeleton in the cell shape changes and force generation in one such model system. Porcine periodontal ligament fibroblasts (PPLF) were cultured within three-dimensional collagen gels attached to glass coverslips. Fluorescence microscopy, using nitrobenzooxadizole (NBD)-phallacidin labeling for microfilaments and tubulin antibody staining for microtubules, was combined with phase and Nomarski optics to determine the intra- and extracellular architecture of the cells and collagen fibers. Samples were observed from 30 minutes to 24 hours after initiation of cell attachment. During attachment and spreading, NBD-phallacidin staining changed dramatically until large microfilament bundles became prominent. Collagen fiber alignment, compaction, and finally tearing from the coverslip occurred during this time. After release of tension, microfilament bundles were no longer evident. The change in microtubule distribution during these processes was less dramatic, appearing to follow the change in cell shape. These results indicate that microfilaments play an essential role in generating force to align and compact collagen, while microtubules may have a secondary role only.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cm.970040105
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  • 7
    Electronic Resource
    Electronic Resource
    Cellular expression of bone-related proteins during in vitro osteogenesis in rat bone marrow stromal cell cultures (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 158 (1994), S. 555-572 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Rat bone marrow stromal cells comprise a heterogeneous mixture of cell lineages including osteoblastic cells. When grown in the presence of ascorbic acid, β-glycerophosphate and 10-8M dexamethasone, osteoprogenitor cells within the population divide and differentiate to form bone nodules (Maniatopoulos et al., 1988, Cell Tissue Res., 254:317-330; Aubin et al., 1990, J. Bone Miner. Res., 5:S81) providing a useful model to investigate temporal and spatial changes in expression of osteoblastic markers. Immunocytochemistry was combined with Northern blotting, enzymatic assay, and radioimmunoassay to analyze the expression of bonerelated proteins during the growth and differentiation sequence. By mRNA levels, protein production and/or enzymatic activity, expression of osteocalcin, bone sialoprotein, and alkaline phosphatase increased concomitantly with the development of bone nodules, while osteopontin mRNA levels decreased and those of SPARC/osteonectin did not change significantly. In older cultures with mineralizing nodules, mRNA levels for alkaline phosphatase and bone sialoprotein, but not osteocalcin, declined. Immunolabeling revealed that cells in early cultures stained poorly for SPARC/osteonectin and strongly for thrombospondin. Later, SPARC/osteonectin staining increased in most cells, while thrombospondin staining could be seen in both matrix and in cells, but with marked intercellular variability in intensity. At all time points studied, osteoblasts within bone nodules stained homogeneously for thrombospondin and alkaline phosphatase, and with marked heterogeneity of intensity amongst cells for SPARC/osteonectin and osteocalcin. Labelling with RCC455.4, a monoclonal antibody raised against rat calvaria cells which Intensely labels osteoblasts and osteocytes (Turksen et al., 1992, J. Histochem. Cytochem., 40:1339-1352), co-localized with osteocalcin. Alkaline phosphatase activity and the amount of osteocalcin determined by both radioimmunoassay and immunolabelling decreased in very late cultures, a time corresponding to appearance of fully mineralized nodules. These studies indicate that the bone marrow stromal cell system is a useful model to study the temporal and spatial expression of bone-related proteins during osteogenesis and formation, mineralization, and maturation of bone nodules. Further, immunolabelling at the individual cell and single bone nodule level allowed discrimination of marked variability of expression of osteoblast markers during the differentiation sequence. © 1994 Wiley-Liss, Inc.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041580322
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  • 8
    Electronic Resource
    Electronic Resource
    Studies on the alignment of fibroblasts in uniform applied electrical fields (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 10 (1989), S. 371-384 
    Details
    ISSN: 0197-8462
    Keywords: human gingival fibroblasts ; statistics of directional data ; Ca2+ transport ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Uniform electrical fields have been applied to human gingival fibroblasts by means of uniform ionic currents passed through a thin chamber. Cells were observed to align in fields between 0.1 and 1.5 V/mm but did not display directed motion toward the anode or the cathode of the chamber. Statistical analysis of directional data was used to distinguish threshold levels of orientation at low field intensities, to quantify the dependence of alignment on time and field intensity, and to analyze differences between alignment of cells treated with the Ca2+ transport modifiers A23187, verapamil, and lanthanum. Alignment occurred at a steady rate and was dependent in a saturating fashion on field strength. The Ca2+ ionophore A23187 had a significant inhibitory effect on cell alignment in applied electrical fields; however, the Ca2+ channel blockers lanthanum and verapamil did not have a significant effect on alignment.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250100406
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  • 9
    Electronic Resource
    Electronic Resource
    Phenotypic differences in subclones and long-term cultures of clonally derived rat bone cell lines (1986)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 31 (1986), S. 153-169 
    Details
    ISSN: 0730-2312
    Keywords: collagens ; extracellular matrix ; bone cells ; cell clones ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Previous studies with clonally derived populations of cells have shown that cells released from embryonic rat calvaria by enzymatic digestion are heterogeneous with respect to their hormone responsiveness, morphology, and production of matrix components [Aubin JE et al; J. Cell Biol 92:452, 1982].Several of these clonal populations have been used to study the effects of long-term culture and inter- and intraclonal cell heterogeneity. During continuous subculture, marked changes in collagen synthesis were observed in two clonal populations. Both of these clones were originally responsive to parathyroid hormone (PTH) and synthesized primarily type I collagen with small amounts of type III and V collagens, although one clone (RCJ 3.2) had a fibroblastic morphology whereas the second clone (RCB 2.2) displayed a more polygonal shape. Following routine subculture over 3 yr, clone RCB 2.2 was found to synthesize exclusively αl(I)-trimer and not other interstitial collagens. When the same cells were maintained at confluence for 1-2 wk, however, they also synthesized type III collagen. Whereas RCJ 3.2 did not show such dramatic changes in collagen synthesis after long-term subculture, two subclones derived from RCJ 3.2 were found to synthesize almost exclusively either type III collagen (RCJ 3.2.4.1) or type V collagen (RCJ 3.2.4.4). Immunocytochemical staining indicated that both subpopulations also produced type IV collagen, laminin, and basement membrane proteoglycan, proteins that are typically synthesized by epithelial cells. The differences in collagen expression by the various clonal cell populations were accompanied by qualitative and quantitative differences in other secreted proteins and differences in cell morphology. The results demonstrate both the inter- and intraclonal heterogeneity of connective tissue cells and their diverse potentiality with respect to extracellular matrix synthesis.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240310207
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  • 10
    Electronic Resource
    Electronic Resource
    Bone stem cells (1998)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 72 (1998), S. 73-82 
    Details
    ISSN: 0730-2312
    Keywords: osteoblast ; bone ; stem cells ; osteoprogenitor ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Osteoblasts are the skeletal cells responsible for synthesis, deposition, and mineralization of the extracellular matrix of bone. By mechanisms that are only beginning to be understood, stem and primitive osteoprogenitors and related mesenchymal precursors arise in the embryo and at least some appear to persist in the adult organism, where they contribute to replacement of osteoblasts in bone turnover and in fracture healing. In this paper, the nature of these cells, whether they constitute a stem cell pool or a committed progenitor pool, and aspects of their apparent plasticity are discussed. Current understanding of differential expression of osteoblast-associated genes during osteoprogenitor proliferation and differentiation to mature matrix synthesizing osteoblasts is summarized. Finally, evidence is discussed that supports the hypothesis that the mature osteoblast phenotype is heterogeneous with subpopulations of osteoblasts expressing only subsets of the known osteoblast markers, raising also the possibility of multiple parallel differentiation pathways and perhaps even different progenitor pools. J. Cell. Biochem. Suppls. 30/31:73-82, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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