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  • 1
    Electronic Resource
    Electronic Resource
    Synthesis of azido tubulin: a photoaffinity label for tubulin-binding proteins (1989)
    s.l. : American Chemical Society
    Biochemistry 28 (1989), S. 8490-8496 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00447a033
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  • 2
    Electronic Resource
    Electronic Resource
    Conference Summary (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 466 (1986), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1986.tb38484.x
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  • 3
    Electronic Resource
    Electronic Resource
    Microtubule Organizing Centers (1985)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Cell and Developmental Biology 1 (1985), S. 145-172 
    Details
    ISSN: 0743-4634
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.cb.01.110185.001045
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  • 4
    Electronic Resource
    Electronic Resource
    COLD-LABILE AND COLD-STABLE MICROTUBULES IN THE MITOTIC SPINDLE OF MAMMALIAN CELLS (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 253 (1975), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1975.tb19218.x
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  • 5
    Electronic Resource
    Electronic Resource
    FLUORESCENTLY-LABELED CALMODULIN LOCALIZES SPECIFIC SITES ON MITOCHONDRIA (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 356 (1980), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1980.tb29643.x
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  • 6
    Electronic Resource
    Electronic Resource
    Movement and segregation of kinetochores experimentally detached from mammalian chromosomes (1988)
    [s.l.] : Nature Publishing Group
    Nature 336 (1988), S. 251-254 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] When Chinese hamster cells were arrested at the Gl/S boun-dary of the cell cycle by treatment with 2 mM hydroxyurea for 20 h and subsequently treated for 4-6 h with caffeine (5 mM), the cells entered mitosis without completing DNA synthesis as originally observed in BHK (baby hamster kidney) ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/336251a0
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  • 7
    Electronic Resource
    Electronic Resource
    The cenpB gene is not essential in mice (1998)
    Springer
    Chromosoma 107 (1998), S. 570-576 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract.  Centromere protein B (CENP-B) is a centromeric DNA-binding protein that binds to α-satellite DNA at the 17 bp CENP-B box sequence. The binding of CENP-B, along with other proteins, to α-satellite DNA sequences at the centromere, is thought to package the DNA into heterochromatin subjacent to the kinetochore of mitotic chromosomes. To determine the importance of CENP-B to kinetochore assembly and function, we generated a mouse null for the cenpB gene. The deletion removed part of the promoter and the entire coding sequence except for the carboxyl-terminal 35 amino acids of the CENP-B polypeptide. Mice heterozygous or homozygous for the cenpB null mutation are viable and healthy, with no apparent defect in growth and morphology. We have established mouse embryo fibroblasts from heterozygous and homozygous cenpB null littermates. Microscopic analysis, using immunofluorescence and electron microscopy of the cultured cells, indicated that the centromere-kinetochore complex was intact and identical to control cells. Mitosis was identical in fibroblasts derived from cenpB wild-type, heterozygous and null animals. Our studies demonstrate that CENP-B is not required for the assembly of heterochromatin or the kinetochore, or for completion of mitosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004120050343
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  • 8
    Electronic Resource
    Electronic Resource
    The fine structure of the kinetochore of a mammalian cell in vitro (1966)
    Springer
    Chromosoma 19 (1966), S. 28-43 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The chromosomes of Chinese hamster cells were examined with the electron microscope and the following observations were made concerning the structure and organization of the kinetochore. — The kinetochore consists of a dense core 200–300 Å in diameter surrounded hy a less dense zone 200–600 Å wide. The dense core consists of a pair of axial fibrils 50–80 Å in diameter which may be coiled together in a cohelical manner. The less dense zone about the axial elements is composed of numerous microfibrils which loop out at right angles to the axial fibrils. Together the structures comprise a lampbrush-like filament which extends along the surface of each chromatid. Some sections suggested that two such filaments may be present on each chromatid. The fine structure of kinetochores associated with spindle filaments was essentially the same as those free of filaments. The structure and organization of the kinetochore of these mammalian cells was compared to that of lampbrush chromosomes of certain amphibian oöcytes, dipteran polytene chromosome puffs, and the synaptinemal complex seen during meiotic prophase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332792
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  • 9
    Electronic Resource
    Electronic Resource
    Localization of tubulin in the mitotic apparatus of mammalian cells by immunofluorescence and immunoelectron microscopy (1977)
    Springer
    Chromosoma 60 (1977), S. 223-235 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Antitubulin antibody was used as an immunofluorescent and immunoelectron microscopic probe to localize tubulin in components of the mitotic apparatus of rat kangaroo (strain PtK1) cells in vitro. In addition to the detection of tubulin in the spindle microtubules and centrioles, other structures were found to display specific staining including kinetochores, amorphous pericentriolar material and small virus-like particles associated with the centrioles. The kinetochores consisted of a densely stained outer layer about 400 Å thick which is separated from an inner layer of the same dimension by a lightly staining middle layer. Microtubules were primarily associated with the outermost plate of the kinetochore but tubulin was uniformly distributed in both outer and inner plates. Colcemid treatment prevented the assembly of spindle microtubules and resulted in specific alterations of the kinetochore but failed to diminish the staining of the kinetochores. These observations suggest that tubulin molecules may comprise an important structural component of the kinetochore.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329772
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  • 10
    Electronic Resource
    Electronic Resource
    Arrangements of kinetochores in mouse cells during meiosis and spermiogenesis (1986)
    Springer
    Chromosoma 94 (1986), S. 309-317 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Antibodies from the serum of patients with the autoimmune disease scleroderma CREST were used to investigate the association and distribution of kinetochores in mouse cells during meiosis and spermiogenesis. The pattern of indirect immunofluorescent staining in pachytene nuclei indicated that each autosomal bivalent contains one fluorescent spot. Throughout pachytene, the kinetochores were arranged non-randomly into several clusters and distributed around the periphery of the nucleus. In subsequent stages of meiotic prophase I, distribution was random and the number of fluorescent spots increased from 21 to 40 corresponding to the diploid chromosome number and the number of halfbivalents oriented to the spindle poles at the metaphase I. Twenty pairs of kinetochores were observed at metaphase II. During spermiogenesis, the number of kinetochores correlated with the haploid chromosome number in early spermatids but tandem association of centromeres and clustering into a conspicuous chromocenter corresponded to a significant reduction in the number of fluorescent foci in mid-spermatid nuclei. The number of stained sites per nucleus continued to decrease during sperm maturation and total absence of staining was apparent in mature spermatozoa. Immunoblotting of proteins extracted from mature sperm however, indicated that a kinetochore antigen of Mr 80,000 was still present. Therefore, the absence of kinetochore staining in mature spermatozoa is probably due to the blockage of epitopes during chromatin condensation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290861
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