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  • 1
    Electronic Resource
    Electronic Resource
    Crossed Immunoelectrophoretic Analysis of Precipitins to Candida albicans in Deep Candida Infection (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 4 (1975), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: By means of crossed immunoelectrophoresis with intermediate gel the precipitins to C. albicans in sera from 18 patients with deep Candida infection were compared with the findings in a control material consisting of sera from 169 'normal' controls (mainly healthy blood donors), 25 patients with oral candidiasis, 14 patients with vaginal candidiasis, and 6 persons without candidiasis giving false positive reactions by the double diffusion method. Up to 20 different precipitins were found in individual sera, and precipitins were demonstrated in all sera except in 6 'normal' controls. Of 692 precipitins 682 (98%) were identified and titrated in terms of the 78-component reference system described earlier for C. albicans. No free Candida antigens were found, but would have been detected by the method. The best criterion for discrimination between sera from patients and controls was the precipitin score, which accounts for number of precipitins and for titres. A precipitin score of more than 6 1/2 had a diagnostic sensitivity of 88.9% and a specificity of 99.5%. Eleven precipitin specificities were found solely in the patients' sera, but the highest sensitivity for a single precipitin was 33%. Until more evidence has accumulated in favour of a monospecific immunoassay it is therefore rational to use a complex antigen extract for further evaluations of diagnostic Candida serology and a short cut to standardization would be the introduction of the method used in the present study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1975.tb03830.x
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and sequence of the gene encoding ornithine decarboxylase, SPE1, from Candida albicans by complementation of a spe1Δ ctrain of Saccharomyces cerevisiae (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 13 (1997), S. 1383-1389 
    Details
    ISSN: 0749-503X
    Keywords: ornithine decarboxylase ; SPE1 gene ; Candida albicans ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The gene encoding ornithine decarboxylase, SPE1, from the pathogenic yeast Candida albicans has been isolated by complementation of an ornithine decarboxylase-negative (spe1Δ) strain of Saccharomyces cerevisiae. Four transformants, three of which contain plasmids with the SPE1 gene, were isolated by selection on polyamine-free medium. The C. albicans ornithine decarboxylase (ODC) showed high homology with other eukaryotic ODCs at both the amino acid and nucleic acid levels. The GenBank accession number for this gene is U85005. © 1997 John Wiley & Sons, Ltd.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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