ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
In this work, we developed a novel patterned co-culture method with thermo-responsivepoly(N-isopropylacrylamide) (PIPAAm) and poly(N-ρ-vinylbenzyl-Ο-β-D-galactopyranosyl-(1→4)-D-gluconamide) (PVLA) inducing active hepatocyte attachment. Patterned graft of PIPAAmonto PS dishes was carried out by electron beam irradiation using cover-glass as a photomask.PVLA was only coated onto PIPAAm-ungrafted domain because of hydrated hydrophilic propertyof PIPAAm at below the LCST. Analysis by attenuated total reflection-Fourier transform infraredand electron spectroscopy for chemical analysis revealed that PIPAAm and PVLA weresuccessfully grafted and coated on surfaces of PS dishes. PIPAAm-grafted surface exhibiteddecreasing contact angle by changing temperature from 37 to 20°C, while PVLA-coated PS andnon-treated PS had negligible contact angle changes with temperature alternation. Atomic forcemicroscopy (AFM) results showed that PIPAAm-grafted and PVLA-coated PS had smoothersurfaces than that of ungrafted PS dishes. After culture for 12 hours, hepatocytes were well attachedon PVLA-coated domain. Hepatocytes adherent on PIPAAm-grafted domain were detached bydecreasing temperature. And then, fibroblasts were seeded onto PIPAAm pattern-grafted domain.Fibroblasts were only attached and spread onto PIPAAm-grafted domain. Co-cultured hepatocytesshowed better differentiated function of albumin expression compared to homotypic hepatocyteculture
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/54/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.342-343.221.pdf
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