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  • 1
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Superoxide dismutase (SOD) catalyzes the nitration of specific tyrosine residues in proteins by peroxynitrite (ONOO−), which may be the damaging gain-of-function resulting from mutations to SOD associated with familial amyotrophic lateral sclerosis (ALS). We found that disassembled neurofilament-L (light subunit) was more susceptible to tyrosine nitration catalyzed by SOD in vitro. Neurofilament-L was selectively nitrated compared with the majority of other proteins present in brain homogenates. Assembled neurofilament-L was more resistant to nitration, suggesting that the susceptible tyrosine residues were protected by intersubunit contacts in assembled neurofilaments. Electrospray mass spectrometry of trypsin-digested neurofilament-L showed that tyrosine 17 in the head region and tyrosines 138, 177, and 265 in α-helical coil regions of the rod domain of neurofilament-L were particularly susceptible to SOD-catalyzed nitration. Nitrated neurofilament-L inhibited the assembly of unmodified neurofilament subunits, suggesting that the affected tyrosines are located in regions important for intersubunit contacts. Neurofilaments are major structural proteins expressed in motor neurons and known to be important for their survival in vivo. We suggest that SOD-catalyzed nitration of neurofilament-L may have a significant role in the pathogenesis of ALS.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 41 (1993), S. 1961-1967 
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 42 (1994), S. 2466-2474 
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Deep-sea sediments become apparently more hostile to life with increasing depth as temperature and pressure rise, and organic matter becomes increasingly recalcitrant. Demonstrations of high bacterial populations in deep sediments, may thus appear enigmatic. How, then, can the continued ...
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Fractionation of total adult DNA of five of the seven species of the melanogaster species sub-group of Drosophila in actinomycin D and distamycin A caesium density gradients has revealed the presence of three main-band DNA components, common to all species, and ten satellite DNAs that are distributed between the species. Satellite DNAs are either unique to a species or common to two or more species. The abundance of a common satellite DNA varies between species. There is no simple relationship between the presence of a satellite DNA and a branch point of phylogenetic divergence; nevertheless the arrangement of the species in a phylogeny that is based on the numbers of satellites held in common accurately reflects the pattern of relationships between the same species based on differences in inversions of polytene chromosomes. The species can be similarly arranged according to the compositions of their mitochondrial DNAs. It is possible that the same basic set of sequences, each of low frequency, is common to all species with arbitrary or selected amplification of particular sequences to differing extents in individual species. The conservation of satellites in the group and the close parallel between the distributions of satellites and inversions between the species suggests that either the processes that operate to change both chromosomal phenomena are similarly time-dependent and occurring at relatively low rates or that their rates of change are restricted according to some undetermined functions of these aspects of the genome.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have investigated the organisation, nucleotide sequence, and chromosomal distribution of a tandemly repeated, satellite DNA from Allium cepa (Liliaceae). The satellite, which constitutes about 4% of the A. cepa genome, may be resolved from main-band DNA in antibiotic-CsCl density gradients, and has a repeat length of about 375 base pairs (bp). A cloned member of the repeat family hybridises exclusively to chromosome telomeres and has a non-random distribution in interphase nuclei. We present the nucleotide sequences of three repeats, which differ at a large number of positions. In addition to arrays made up of 375-bp repeats, homologous sequences are found in units with a greater repeat length. This divergence between repeats reflects the heterogeneity of the satellite determined using other criteria. Possible constraints on the interchromosomal exchange of repeated sequences are discussed.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Contributions to mineralogy and petrology 93 (1986), S. 524-531 
    ISSN: 1432-0967
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract A series of calculations have been carried out to evaluate the effect on cumulus mineral compositions of solidification of trapped intercumulus liquid in orthocumulates. The calculation assumes local equilibrium between phases, and that the system remains chemically closed during crystallization of the trapped liquid. The latter assumption is held to be valid on a scale of tens to hundreds of centimeters. It is not necessary to know the composition of the trapped liquid, as the calculation only requires an estimate of FeO content and trapping temperature. The change in composition of a mineral from that of the initially precipitated cumulus crystals to the final composition after complete solidification is termed the “trapped liquid shift”. Its magnitude depends on the modal proportions of cumulus phases and the initial porosity, and is only weakly dependent on initial phase compositions. Trapped liquid shifts are significant when compared with mineral composition changes occurring during fractional crystallization. Crystallization of 30% trapped liquid gives rise to shifts of up to 10 mol. percent in Mg number of olivine or pyroxene. The size of the shift becomes greater when the initial cumulus assemblage has a lower total FeO+MgO content, and vice versa. As a result of the relationship between trapped liquid shift and cumulus mode, mineral composition variations and trends may be generated in sequences of cumulates which originally had constant compositions of cumulus minerals. For example, in a cyclic unit grading from a pyroxenitic base to an anorthositic top, crystallization of a uniform proportion of trapped liquid will result in an apparent iron enrichment trend from bottom to top of the cycle, as has been observed in the Upper Critical Zone of the Bushveld Complex.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Contributions to mineralogy and petrology 105 (1990), S. 704-714 
    ISSN: 1432-0967
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Spinifex-textured komatiites at Honeymoon Well, Western Australia, show evidence of partial melting and recrystallization of original igneous textures. Their textures and mineral compositions differ markedly from those typical of komatiites. Spinifex olivine plates are bent and broken, while interstitial space between spinifex and cumulus olivine is occupied by polygonal aggregates of clinopyroxene, orthopyroxene, minor olivine and plagioclase. Similar granular pyroxene-plagioclase aggregates occur as diffuse veins cutting spinifex zones and cumulate zones of the flows and, in places, form the matrix to a breccia containing corroded fragments of spinifex rock. Thermometry based on the two pyroxene assemblages yields temperatures of 1055° to 1141° C, just below the low-pressure komatiite solidus. Mineral compositions are different from those of typical komatiites: clinopyroxenes are Al-poor and Cr-rich, olivines are unusually iron-rich and depleted in Cr and Ca, and the low-Ca pyroxene is bronzite rather than the more typical pigeonite. We interpret these observations as the results of thermal metamorphism, partial remelting and subsequent slow crystallization of originally normal spinifex-textured komatiite flows. The rocks in question occupy a 40–70 m interval sandwiched between two olivine-rich units: an underlying 90 m-thick olivine adcumulate layer, forming part of the cumulate zone of a basal 160 m-thick flow, and an overlying 1 km-thick extrusive body composed mostly of olivine mesocumulate and adcumulate and capped in turn by spinifex-textured flows. Thermal modelling shows that a sinusoidal temperature profile of cool flow tops and hot flow centres would exist within this sequence shortly after eruption. Conductive thermal relaxation of this profile could reheat spinifex zones to the extent of inducing partial melting and textural reconstitution. Such reheating is largely dependent on the time interval between the emplacement of successive flows. Calculations suggest that at Honeymoon Well the emplacement interval must have been of the order of 10 years or less. Textural reconstitution may have contributed to the development of the thick orthocumulate sequences characteristic of komatiites in the Agnew-Wiluna belt.
    Type of Medium: Electronic Resource
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