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  • 1
    Digitale Medien
    Digitale Medien
    Mode of replication, size and distribution of naturally occurring plasmids in Bacillus thuringiensis (1992)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 96 (1992), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Abstract Cloned replication origin regions, derived from both small (4.9–7.5 MDa) and large (43–60 MDa) plasmids of Bacillus thuringiensis subspecies kurstaki strains HD73 and HD263 were used as hybridization probes in a Southern-blot analysis to assess both the size and horizontal distribution of native plasmid replicon groups among different subspecies of B. thuringiensis. In general, resident plasmids hybridizing to the replication origin regions derived from strains HD263 and HD73 were more commonly found in kurstaki strains than in non-kurstaki strains, suggesting a non-random distribution of plasmid incompatibility groups. Replication origin regions derived from the large HD263 plasmids (43–60 MDa) hybridized almost exclusively with large plasmids (〉30 MDa) of widely varying sizes. In contrast, replication origin regions derived from small plasmids hybridized exclusively with small plasmids (〈 10 MDa) showing little size variation. These results are consistent with previous observations concerning the relationship between plasmid size, mode of replication, and structural stability.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1992.tb05407.x
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  • 2
    Digitale Medien
    Digitale Medien
    Regulation of insecticidal crystal protein production in Bacillus thuringiensis (1995)
    Osney Mead, Oxford OX2 0EL, UK : Blackwell Science Ltd
    Molecular microbiology 18 (1995), S. 0 
    Details
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: The production of insecticidal crystal proteins (ICPs) in Bacillus thuringiensis normally coincides with sporulation, resulting in the appearance of parasporal crystalline inclusions within the mother cell. In most instances, the temporal and spatial regulation of ICP gene expression is determined at the transcriptional level by mother-cell-specific sigma factors that share homology with σE and σK from Bacillus subtilis. The crylll ICP genes are a notable exception; these genes are transcribed from σA-like promoters during vegetative growth, are induced or derepressed at the onset of stationary phase, and are overexpressed in sporulation mutants of B. thuringiensis blocked in the phosphorylation of Spo0A, a key regulator of sporulation initiation. Transcription alone, however, cannot account for the impressive ability of this bacterium to accumulate insecticidal proteins. A variety of post-transcriptional and post-translational mechanisms also contribute to the efficient production of ICPs in B. thuringiensis, thus making this bacterium a cost-effective biological control agent.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2958.1995.mmi_18010001.x
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  • 3
    Digitale Medien
    Digitale Medien
    Control of coleopteran insect pests through RNA interference (2007)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 25 (2007), S. 1322-1326 
    Details
    ISSN: 1546-1696
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: [Auszug] Commercial biotechnology solutions for controlling lepidopteran and coleopteran insect pests on crops depend on the expression of Bacillus thuringiensis insecticidal proteins, most of which permeabilize the membranes of gut epithelial cells of susceptible insects. However, insect control strategies ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/nbt1359
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  • 4
    Digitale Medien
    Digitale Medien
    Expression of genetically distinct superoxide dismutases in the maize seedling during development (1982)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 3 (1982), S. 7-23 
    Details
    ISSN: 0192-253X
    Schlagwort(e): gene expression ; superoxide dismutase ; Zea mays ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: Immunoassays for the cytosolic and mitochondrial superoxide dismutases (SOD) of maize were developed and used to study the expression of these proteins in the maize seedling. The genetically distinct proteins, SOD-3 and SOD-4, are preferentially expressed in the scutellum, comprising approximately 1% of the total water-soluble protein of that tissue. SOD-2, SOD-3, and SOD-4 are synthesized in the scutellum during early sporophytic development, probably on cytosolic ribosomes. Two-dimensional gel electrophoresis of crude scutellar extracts indicates that significant changes occur in the protein composition of the maize scutellum following seed imbibition. Using the immunoassays, a maize line exhibiting a significant reduction in cyanide-sensitive SOD protein was identified.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/dvg.1020030103
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