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  • 1
    Electronic Resource
    Electronic Resource
    Stability of RNA transcripts in post-mortem psychiatric brains (1999)
    Springer
    Journal of neural transmission 106 (1999), S. 329-335 
    Details
    ISSN: 1435-1463
    Keywords: Keywords:β-actin ; β2-microglobulin ; competitive RT-PCR ; GAPDH ; post-mortem ; RNA ; Trk B ; Trk C.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary. RNA isolated from frozen human post-mortem brain tissue was used for analysis of five gene products with a recently developed sensitive and competitive RT-PCR technique. Samples varying in post-mortem intervals up to four days from controls, schizophrenics and alcoholics were analyzed. Evaluation of three housekeeping genes, as well as Trk B and Trk C demonstrated that the levels of mRNA transcripts were stable in brain samples at all time periods (one to four days) examined. This observation demonstrates that this RT-PCR protocol is a sensitive and reliable method to study relative amounts of mRNAs. The overall stability of housekeeping transcripts implicates the value of post-mortem brain samples for differential gene expression studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007020050162
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Plaque formation in brain transplants exposed to human β-amyloid precursor protein 695 (1996)
    Springer
    Acta neuropathologica 92 (1996), S. 130-137 
    Details
    ISSN: 1432-0533
    Keywords: Key words Plaque formation ; β-Amyloid precursor ; protein  ; Alzheimer’s disease ;  Transplantation ; βA4 peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Abnormal processing of β-amyloid precursor protein (APP) and precipitation of amyloidogenic βA4 peptide have been implicated in the pathogenesis of Alzheimer’s disease (AD). In an attempt to generate an in vivo model of APP-associated pathology, we have introduced recombinant retroviral vectors harboring normal and mutant cDNAs for human neuron-specific APP 695 into fetal rat brain transplants. A minor population of recombinant neural cells with expression of APP was identified immunohistochemically in the grafts. The expression was maintained during an observation period of 9 months. Neurons with strong immunoreactivity for human APP exhibited markedly swollen perikarya and showed signs of cell degeneration. At 6 months post-transplantation, recombinant grafts developed APP-positive neuropil deposits with morphological features of neuritic plaques, but without βA4 peptide immunoreactivity. No difference was observed between wild-type APP and the mutant APP construct derived from a family with autosomal dominant AD. These observations indicate that long-term neuronal overexpression of human APP has the potential to generate APP plaques in the rodent brain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050500
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Hippocampal loss of N-methyl-D-aspartate receptor subunit 1 mRNA in chronic temporal lobe epilepsy (1995)
    Springer
    Acta neuropathologica 89 (1995), S. 446-450 
    Details
    ISSN: 1432-0533
    Keywords: Key wordsN-Methyl-D-aspartate receptor ; Epilepsy ; Non-radioactive in situ hybridization ; Hippocampus ; Ammon's horn sclerosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The hippocampal distribution of mRNA for the N-methyl-D-aspartate (NMDA) receptor subunit 1 (NR1) was examined by non-radioactive in situ hybridization in 21 archival formalin-fixed and paraffin-embedded surgical specimens from patients with pharmacoresistant chronic epilepsy and in normal control specimens obtained at autopsy. Using the digoxigenin-labeling procedure, ribonucleotide probes were found to be significantly more sensitive than synthetic oligonucleotide probes. In normal autopsy specimens and in surgical specimens without Ammon's horn sclerosis there was intense NR1 expression in a great majority of the dentate gyrus granular cells. Many neurons in the hippocampal pyramidal cell layer also revealed a strong signal intensity. The strata oriens and moleculare of Ammon's horn and the molecular layer of the dentate gyrus contained only few labeled neurons. In the subiculum and entorhinal cortex most neurons throughout various layers were positive. In hippocampal specimens of patients with chronic epilepsy there was a loss of NR1-positive cells that was closely related to the overall neuronal loss in the respective specimen and to Ammon's horn sclerosis. These data suggest that the loss of NR1 expression is a secondary phenomenon rather than an event that is relevant for the pathogenesis of epileptic seizures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307650
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Reverse relationship between β-amyloid precursor protein and β-amyloid peptide plaques in Down’s syndrome versus sporadic/familial Alzheimer’s disease (1999)
    Springer
    Acta neuropathologica 97 (1999), S. 113-118 
    Details
    ISSN: 1432-0533
    Keywords: Keywords Alzheimer’s disease ; Down’s syndrome ; β-Amyloid precursor protein ; β-Amyloid peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Strong genetic evidence has been accumulated in favor of a central role of β-amyloid precursor protein (APP) and β-amyloid peptide (βA4) in the pathogenesis of Alzheimer’s disease (AD). We employed four newly developed APP and βA4 antibodies and performed a comparative neuropathological study of patients with Down’s syndrome (DS), early-onset familial AD and sporadic AD to investigate the distribution of APP and βA4 plaque densities in the cerebral cortex of these disorders. Quantitative analysis of APP versus βA4 plaques revealed that brains with early-onset familial AD and sporadic AD showed significantly more βA4 plaques than brains with DS (P 〈 0.05). In contrast, APP plaques were more abundant in DS cerebral cortex (P 〈 0.02). These observations suggest that the development of pathological changes in DS brains does not parallel that observed in AD, which might be attributable to different causes in the pathogenesis of βA4 formation. A comparison of these disorders may be useful to further complement our knowledge of the mechanisms leading to plaque development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050963
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Migratory potential of transplantable neural tumor cell lines (1999)
    Springer
    Acta neuropathologica 97 (1999), S. 607-612 
    Details
    ISSN: 1432-0533
    Keywords: Key words Transplantation ; Migration ; Rat brain ; Matrix-metalloproteinases ; Neural tumor cell lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Experimentally induced primitive neuroectodermal tumor (PNET) cell lines were transplanted into neonatal and adult rat brain and examined neuropathologically for their tumorigenic potential. Both cell lines showed a striking migratory behavior in both neonatal and adult brain. Migration of tumor cells was found in host brain parenchyma, along white matter tracts and associated with CSF pathways. These neural tumor cell lines provide a valuable tool for the development of strategies against strongly migrating neural tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010051036
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    Paper (German National Licenses)
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