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  • 1
    Electronic Resource
    Electronic Resource
    SV40 recombinants carrying a d(CT . GA)"2"2 sequence show increased genomic instability
    Amsterdam : Elsevier
    Gene 108 (1991), S. 269-274 
    Details
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; SV40 variants ; simple repeating DNA ; triple-stranded DNA
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0378-1119(91)90444-G
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  • 2
    Electronic Resource
    Electronic Resource
    New hope of reducing the costs of mass rearing whitefish, Coregonus lavaretus L., larvae on a dry diet? (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 22 (1991), S. 0 
    Details
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2109.1991.tb00529.x
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  • 3
    Electronic Resource
    Electronic Resource
    Application of Factor Analysis to the Study of Contamination in the Aquifer System of Ayamonte-Huelva (Spain) (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 34 (1996), S. 0 
    Details
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: The growth of cultivation (strawberries and citrus fruits) in the coastal region of the Huelva province of Spain in the last 10 years has meant an increase in the demand of water from the Ayamonte-Huelva aquifer system. It has also meant the introduction of an excess of nitrogen via fertilizers from farming.The system now has two problems: salt-water intrusion in the southern sector (González and Romero, 1991; González, 1992); and a general increase in the nitrate content in the saturated zone. This second problem has led to average concentrations of nitrates in the aquifer above potable limits (50 ppm), reaching local values near to 400 ppm of NO3-(González and Romero, 1991; Grande et al., 1993).This paper delineates the sphere of influence of the two factors being considered (salt-water intrusion and agricultural contamination) on the different variables studied. The proximity between both factors is justified for each of the contaminating processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6584.1996.tb01875.x
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  • 4
    Electronic Resource
    Electronic Resource
    Prevalence of antibodies to Rickettsia conorii Coxiella burnetii and Rickettsia typhi in Salamanca province (Spain). Serosurvey in the human population (1990)
    Springer
    European journal of epidemiology 6 (1990), S. 293-299 
    Details
    ISSN: 1573-7284
    Keywords: Rickettsiosis ; Serosurvey ; Rickettsia conorii ; Coxiella burnetii ; Rickettsia typhi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The Salamanca Province of Spain is an endemic zone for Mediterranean spotted fever. In this area, only one case of Q fever has been reported and typhus group rickettsial diseases have never been diagnosed. To obtain a panoramic view of the presence of antibodies to the most ubiquitous rickettsial agents, 400 sera specimens from a statistically representative sample of the human population of Salamanca City and its surrounding province were subjected to indirect microimmunofluorescent test for antibodies against Rickettsia conorii, Coxiella burnetii, phase II, and Rickettsia typhi antigens. Titers ≥1:40 to R. conorii were found in 73.5% of the sera. Positivity was more common in subjects who reported closer contact with the natural environment and/or with domestic animals. Seropositivity to C. burnetii, phase II, was detected in 50.2% of sera. These positive cases were related to rural environmental factors and to prevoius contact with animals. The frequency of antibodies increased with age showing a progressive exposure to the rickettsial antigen. The prevalence of antibodies to R. conorii and C. burnetii in the human population of Salamanca Province is higher than that reported from any other geographic zone. The study of antibodies to R. typhi showed that 12.5% of the sera had titers ≥1:40. According to our results, seropositivity to this rickettsial antigen cannot be related to any particular group of population nor interpreted totally as cross reactivity with R. conorii. Our data show a wide distribution of R. conorii and C. burnetii antigens in Salamanca Province, and also indicate the presence of R. typhi antigens in this area.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00150435
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  • 5
    Electronic Resource
    Electronic Resource
    Acid/extraction treatment of bivalves for organotin speciation (1997)
    Springer
    Fresenius' journal of analytical chemistry 357 (1997), S. 1007-1009 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A method is described for leaching of nanogram amounts of monobutyltin (MBT), dibutyltin (DBT), tributyltin (TBT), monophenyltin (MPT), diphenyltin (DPT), triphenyltin (TPT), and monomethyltin (MMT) from bivalves. The procedure is based on soaking the samples in a water-hydrogen bromide mixture (1:1) with magnetic stirring for 30 min followed by extraction with a 0.04% (w/v) tropolone solution in dichloromethane for 2 h. Organotins are determined by GC-FPD after clean-up through a Florisil column and fat hydrolysis by 1mol/L NaOH, followed by derivatization through Grignard pentylation. The method has been applied for an investigation of the occurrence of organotins in bivalves in south-west Spain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160050294
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  • 6
    Electronic Resource
    Electronic Resource
    Use of western blot to analyze the reactivity of sera from patients with mediterranean spotted fever (1992)
    Springer
    European journal of clinical microbiology & infectious diseases 11 (1992), S. 939-942 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The reactivity of antigenic components ofRickettsia conorii with sequentially obtained sera from 20 adult Spanish patients with Mediterranean spotted fever was analyzed by Western blot. The major rickettsial antigens reacting with the serum samples corresponded to molecular weights of 135 and 115 kDa. These antigens constantly exhibited higher staining intensity than the other antigens, and reacted with 100 % and 86.7 %, respectively, of acute sera and with 100 % of convalescent phase samples. Rickettsial lipopolysaccharide antigens reacted with 94.7 % of sera collected in the fourth and fifth week after onset of symptoms. Other major antigens reactive in the blots had molecular sizes of 160, 100, 90 and 60 kDa, and a relatively frequent humoral immune response was also seen to antigens of 80, 73 and 55 kDa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01962380
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  • 7
    Electronic Resource
    Electronic Resource
    Evaluation of ciprofloxacin and doxycycline in the treatment of mediterranean spotted fever (1992)
    Springer
    European journal of clinical microbiology & infectious diseases 11 (1992), S. 427-431 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A comparison was made of the results obtained with ciprofloxacin (750 mg/12 h) and doxycycline (100 mg/12 h), both administered p.o. for one week, in 34 and 36 patients respectively with Mediterranean spotted fever. Apyrexia was achieved after 50.1 ± 34.2 h (mean ± SD) of antibiotic therapy in the group of patients treated with ciprofloxacin and in 55.2 ± 23.3 h in the group treated with doxycycline (no significant statistical differences). Resolution of the remaining signs and symptoms of the disease was achieved faster with ciprofloxacin. Both therapeutic regimens were effective and safe. Although more expensive than doxycycline, ciprofloxacin is a bactericidal compound which is better tolerated and has a lower risk of toxicity and of development of resistance. It can be considered, together with doxycycline, as an antibiotic of first choice in the treatment of Mediterranean spotted fever.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01961857
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  • 8
    Electronic Resource
    Electronic Resource
    Antigens of Rickettsia conorii recognized by seropositive healthy people from Salamanca (Central-West Spain) (1993)
    Springer
    European journal of epidemiology 9 (1993), S. 59-63 
    Details
    ISSN: 1573-7284
    Keywords: Rickettsiosis ; Spotted fever group rickettisiae ; Rickettisia conorii ; Mediterranean spotted fever ; Epidemiology ; Western immunoblotting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The antigenic reactivity in Western immunoblotting assay of individual Rickettsia conorii components with sera of healthy people living in Salamanca Province, an endemic zone of Mediterranean spotted fever, is evaluated. Polypeptides of molecular weights 100 kDa (92.7%), 135 kDa (75.6%), 160 kDa (70.7%) and 115 kDa (48.8%) were recognized by a higher proportion of sera with indirect immunofluorescent antibody test titers ≥ 1:80. Reaction with apparent rickettsial lipopolysaccharide was found in 15 (36.6%) of these samples. The involvement of different rickettsial strains, atypical routes of inoculation, varying content of the inoculum, and host factors may be determinants of the clinical expression of the spotted fever group rickettsial infection in people who produce antibodies reactive with Rickettsia conorii antigens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00463091
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  • 9
    Electronic Resource
    Electronic Resource
    Use of cartridges for speciation of organotin compounds in environmental samples (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Applied Organometallic Chemistry 8 (1994), S. 553-561 
    Details
    ISSN: 0268-2605
    Keywords: Tributyltin ; dibutyltin ; monobutyltin ; triphenyltin ; diphenyltin ; monophenyltin ; speciation ; C18 cartridges ; C8 cartridges ; preconcentration ; water ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The performance of cartridges with different polar and non-polar stationary phases (C18, C8, C2, phenyl) has been investigated for the quantitative separation of butyltin and phenyltin species (TBT, DBT, MBT, MPT, DPT, TPT), and conditions have been established to optimize the separation, using gas chromatography with a flame photometric detector, to characterize the different organotin species. Optimum separation and preconcentration of organotins is based on retention in a C18 cartridge and elution with 2 cm3 of 1% (v/v) HBr and 0.1% tropolone solution in methanol, being successfully applied to seawater. Finally, the possibilities of the cartridges for selective elution and their use in direct non-chromatographic speciation are discussed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aoc.590080703
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  • 10
    Electronic Resource
    Electronic Resource
    Evaluation of atomic fluorescence spectrometry as a sensitive detection technique for arsenic speciation (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Applied Organometallic Chemistry 12 (1998), S. 439-447 
    Details
    ISSN: 0268-2605
    Keywords: high-performance liquid chromatography ; hydride generation ; atomic fluorescence spectrometry ; photo-oxidation ; arsenic speciation ; human urine ; Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The potential of coupling anion-exchange high-performance liquid chromatography, hydride generation and atomic fluorescence spectrometry (HPLC-HG-AFS) for arsenic speciation is considered. The effects of hydrochloric acid and sodium tetrahydroborate concentrations on signal-to-background ratio, as well as argon and hydrogen flow rates, were investigated. Detection limits for arsenite, dimethylarsinic acid (DMA), monomethylarsonic acid (MMA) and arsenate were 0.17, 0.45, 0.30 and 0.38 μg l-1, respectively, using a 20-μl loop. Linearity ranges were 0.1-500 ng for As(III) and MMA (as arsenic), and 0.1-800 ng for DMA and As(V) (as arsenic). Arsenobetaine (AsB) was also determined by introducing an on-line photo-oxidation step after the chromatographic separation. In this case the limits of detection and linear ranges for the different species studied were similar to the values obtained previously for As(V). The technique was tested with a human urine reference material and a volunteer's sample. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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